A/T-rich inverted DNA repeats are destabilized by chaotrope-containing buffer during purification using silica gel membrane technology

BioTechniques

Volumn 35, Issue 4, 2003, Pages 698-702

  Převorovský, Martin ^a   Půta, František ^a  

^a CHARLES UNIVERSITY   (Czech Republic)

Author keywords

[No Author keywords available]

Indexed keywords

BUFFER; DNA FRAGMENT; REPETITIVE DNA; SILICA GEL;

ARTICLE; AT RICH SEQUENCE; CLONING VECTOR; DNA DENATURATION; DNA PURIFICATION; DNA STRUCTURE; EXPRESSION VECTOR; GENE CASSETTE; GENE SEQUENCE; GENETIC STABILITY; MEMBRANE TECHNOLOGY; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; RNA PURIFICATION; RNA TRANSCRIPTION;

UNIDENTIFIED CLONING VECTOR; VECTORS;

EID: 0141995491     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/03354bm06     Document Type: Article

References (8)

1. Fire, A., S. Xu, M. K. Montgomery, S.A. Kostas, S.E. Driver, and C.C. Mello. 1998. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391:806-811.
2. Sharp, P.A. 2001. RNA interference - 2001. Genes Dev. 15:485-490.
3. Elbashir, S.M., W. Lendeckel, and T. Tuschl. 2001. RNA interference is mediated by 21- and 22-nucleotide RNAs. Genes Dev. 15:188-200.
4. Martens, H., J. Novotny, J. Oberstrass, T.L. Steck, P. Postlethwait, and W. Nellen. 2002. RNAi in Dictyostelium: the role of RNA-directed RNA polymerases and double-stranded RNase. Mol. Biol. Cell 13:445-453.
5. Malhotra, P., P.V. Dasaradhi, A. Kumar, A. Mohmmed, N. Agrawal, R.K. Bhatnagar, and V.S. Chauhan. 2002. Double-stranded RNA-mediated gene silencing of cysteine proteases (falcipain-1 and -2) of Plasmodium falciparum. Mol. Microbiol. 45:1245-1254.
6. Folk, P., F. Puta, L. Krpejsova, A. Blahuskova, A. Markos, M. Rabino, and R.P. Dottin. 1996. The homolog of chromatin binding protein Bx42 identified in Dictyostelium. Gene 181:229-231.
7. Skruzny, M., M. Ambrozkova, I. Fukova, K. Martinkova, A. Blahuskova, L. Hamplova, F. Puta, and P. Folk. 2001. Cyclophilins of a novel subfamily interact with SNW/SKIP coregulator in Dictyostelium discoideum and Schizosaccharomyces pombe. Biochim. Biophys. Acta 1521:146-151.
8. Morio, T., H. Urushihara, T. Saito, Y. Ugawa, H. Mizuno, M. Yoshida, R. Yoshino, B.N. Mitra, et al. 1998. The Dictyostelium developmental cDNA project: generation and analysis of expressed sequence tags from the first-finger stage of development. DNA Res. 5:335-340.