Trypsin digestion of proteins on intact immobilized pH gradient strips for surface matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis

Rapid Communications in Mass Spectrometry

Volumn 17, Issue 20, 2003, Pages 2323-2326

  Iyer, Srinivas ^a   Olivares, José ^a  

^a LOS ALAMOS NATIONAL LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DESORPTION; MASS SPECTROMETRY; MOLECULAR BIOLOGY; PEPTIDES; PH;

INTEGRAL PART; MASS SPECTROMETRIC ANALYSIS; MATRIX-ASSISTED LASER DESORPTION-IONIZATION TIME-OF-FLIGHT MASS; MATRIX-ASSISTED LASER DESORPTION/IONIZATION-TIME-OF-FLIGHT MASS SPECTROMETRY; PH GRADIENTS; PROTEOMICS; SURFACE MATRIX; TRYPSIN DIGESTION; TRYPTIC PEPTIDES; TWO-DIMENSIONAL (2D) ELECTROPHORESIS;

ELECTROPHORESIS;

MYOGLOBIN; TRYPSIN;

AMINO ACID SEQUENCE; ARTICLE; DIFFUSION; ISOELECTRIC FOCUSING; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; PROTEOMICS; TWO DIMENSIONAL ELECTROPHORESIS;

EID: 0141989737     PISSN: 09514198     EISSN: None     Source Type: Journal    
DOI: 10.1002/rcm.1199     Document Type: Article

References (19)

1. Washburn MP, Wolters D, Yates JR III. Nat. Biotechnol. 2001; 19: 242.
2. Wolters DA, Washburn MP, Yates JR III. Anal. Chem. 2001; 73: 5683.
3. Wetterhall M, Palmblad M, Hakansson P, Markides KE, Bergquist J. J. Proteome Res. 2002; 1: 361.
4. Rejtar T, Hu P, Juhasz P, Campbell JM, Vestal ML, Preisler J, Karger BL. J. Proteome Res. 2002; 1: 171.
5. Nedelkov D, Nelson R. J. Mol. Recog. 2003; 16: 15.
6. Chaurand P, Schwartz SA, Caprioli RM. Curr. Opin. Chem. Biol. 2002; 6: 676.
7. Chaurand P, Caprioli RM. Electrophoresis 2002; 23: 3125.
8. Xu BJ, Caprioli RM, Sanders ME, Jensen RA. J. Am. Soc. Mass Spectrom. 2002; 13: 1292.
9. Ogorzalek Loo RR, Loo JA, Andrews PC. Methods Mol. Biol. 1999; 112: 473.
10. Ogorzalek Loo RR, Mitchell C, Stevenson TI, Martin SA, Hines WM, Juhasz P, Patterson DH, Peltier JM, Loo JA, Andrews PC. Electrophoresis 1997; 18: 382.
11. Cohen SL, Chait BT. Anal. Biochem. 1997; 247: 257.
12. Loo RR, Cavalcoli JD, VanBogelen RA, Mitchell C, Loo JA, Moldover B, Andrews PC. Anal. Chem. 2001; 73: 4063.
13. Havlis J, Thomas H, Sebela M, Shevchenko A. Anal. Chem. 2003; 75: 1300.
14. Craft D, Doucette A, Li L. J. Proteome Res. 2002; 1: 537.
15. Stensballe A, Jensen ON. Proteomics 2001; 1: 955.
16. Iyer S, Bradbury EM, Olivares JA. Proc. 50th ASMS Conf. Mass Spectrometry and Allied Topics, Orlando, FL, 2002; A020599.
17. Loo JA, Brown J, Critchley G, Mitchell C, Andrews PC, Ogorzalek Loo RR. Electrophoresis 1999; 20: 743.
18. Available: www.badger-airbrush.com.
19. Harris MN, Burchiel SW, Winyard PG, Engen JR, Mobarak CD, Timmins GS. Chem. Res. Toxicol. 2002; 15: 1589.