The impact of single cysteine residue mutations on the replication terminator protein

Biochemical and Biophysical Research Communications

Volumn 310, Issue 4, 2003, Pages 1096-1103

  Vivian, J P ^a   Hastings, A F ^b   Duggin, I G ^b   Wake, R G ^b   Wilce, M C J ^a,c   Wilce, J A ^c  

^a UNIVERSITY OF WESTERN AUSTRALIA   (Australia)

^b UNIVERSITY OF SYDNEY   (Australia)

^c UNIVERSITY OF WESTERN AUSTRALIA   (Australia)

Author keywords

Bacillus subtilis; Biophysical analysis; Cysteine mutagenesis; DNA binding; Protein engineering; Protein structure and function; Replication terminator protein

Indexed keywords

ALANINE; CYSTEINE; MUTANT PROTEIN; PROTEIN; REPLICATION TERMINATOR PROTEIN; SERINE; UNCLASSIFIED DRUG; VALINE;

AMINO ACID SUBSTITUTION; ARTICLE; BACILLUS SUBTILIS; BINDING AFFINITY; CIRCULAR DICHROISM; DNA BINDING; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; POLARIMETRY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ENGINEERING; PROTEIN SECONDARY STRUCTURE; PROTEIN STABILITY; PROTEIN STRUCTURE; PROTEIN VARIANT; SENSITIVITY ANALYSIS; SITE DIRECTED MUTAGENESIS; SOLUBILITY; STRUCTURE ANALYSIS; ULTRACENTRIFUGATION; WILD TYPE; X RAY CRYSTALLOGRAPHY;

BACILLUS SUBTILIS;

EID: 0141961674     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbrc.2003.09.126     Document Type: Article

References (22)

1. Hill T.M. Features of the chromosomal terminus region. Neidhardt F.C., Curtiss III R., Ingraham J.L., Lin E.C., Low K.B., Magasanik B., Reznikoff W.S., Riley M., Schaechter M., Umbarger H.E. Escherichia coli and Salmonella: Cellular and Molecular Biology. 1996;1602-1614 American Society for Microbiology, Washington, DC.
2. Wake R.G. Replication fork arrest and termination of chromosome replication in Bacillus subtilis. FEMS Microbiol. Lett. 153:1997;247-254.
3. Wake R.G., King G.F. A tale of two terminators: crystal structures sharpen the debate on DNA replication fork arrest mechanisms. Structure. 5:1997;1-5.
4. Griffiths A.A., Anderson, Wake R.G. Replication terminator protein-based replication fork-arrest systems in various Bacillus species. J. Bacteriol. 180:1998;3360-3367.
5. Kralicek A.V., Wilson P.K., Ralston G.B., Wake R.G., King G.F. Reorganisation of terminator DNA upon binding replication terminator protein: implications for the functional fork arrest complex. Nucleic Acids Res. 25:1997;590-596.
6. Wilce J.A., Vivian J.P., Hastings A.F., Otting G., Folmer R., Duggin I.G., Wake R.G., Wilce M.C.J. Structure of the RTP/DNA complex and the mechanism of polar replication fork arrest. Nat. Struct. Biol. 8:2001;206-210.
7. Kralicek A.V., Vesper N.A., Ralston G.B., Wake R.G., King G.F. Symmetry and secondary structure of the replication terminator protein of Bacillus subtilis: sedimentation equilibrium and circular dichroic, infrared, and NMR spectroscopic studies. Biochemistry. 32:1993;10216-10223.
8. Yphantis D.A. Equilibrium ultracentrifugation of dilute solutions. Biochemistry. 3:1964;297-317.
9. Johnson M.L., Correia J.J., Yphantis D.A., Halvorson H.R. Analysis of data from the analytical ultracentrifuge by nonlinear least-squares techniques. Biophys. J. 36:1981;575-588.
10. Smith M.T., de Vries C.J., Langley D.B., King G.F., Wake R.G. The Bacillus subtilis DNA replication terminator. J. Mol. Biol. 260:1996;54-69.
11. Duggin I.G., Anderson P.A., Smith M.T., Wilce J.A., King G.F., Wake R.G. Site-directed mutants of RTP of Bacillus subtilis and the mechanism of replication fork arrest. J. Mol. Biol. 286:1999;1325-1335.
12. Mehta P.P., Bussierre D.E., Hoffman D.W., Bastia D., White S.W. Crystallisation ond preliminary structural analysis of the replicator terminator protein of Bacillus subtilis. J. Biol. Chem. 267:1992;18885-18889.
13. Otwinowski Z., Minor W. Processing of X-ray diffraction data collected in oscillation mode. Methods Enzymol. 276:1997;307-326.
14. Collaborative Computational Project, Number 4, The CCP4 Suite: programs for protein crystallography, Acta Cryst. D 50 (1994) 760-763.
15. Bussiere D.E., Bastia D., White S.W. Crystal structure of the replication terminator protein from B. subtilis at 2.6. Å Cell. 80:1995;651-660.
16. Brünger A.T., Adams P., Clore G., DeLano W., Gros P., Grosse-Kunstleve R., Jiang J.-S., Kuszewski J., Nilges M., Pannu N., Read R., Rice L., Simonson T., Warren G. Crystallography and NMR system: a new software suite for macromolecular structure determination. Acta Crystallogr. D. 54:1998;905-921.
17. Jones T.A., Zou J.-Y., Cowan S.W., Kjeldgaard M. Improved methods for building protein models in electron density maps and the location of errors in these models. Acta Crystallogr. A. 47:1991;110-119.
18. Bax A., Ikura M., Kay L.E., Torchia D.A., Tschudin R. Comparison of different modes of two-dimensional reverse correlation NMR for the study of proteins. J. Magn. Reson. 86:1990;304-318.
19. 15N spectroscopy. J. Magn. Reson. 87:1990;488-501.
20. Pai K.S., Bussiere D.E., Wang F., Hutchison III C.A., White S.W., Bastia D. The structure and function of the replication terminator protein of Bacillus subtilis: identification of the 'winged helix' DNA-binding domain. EMBO J. 15:1996;3164-3173.
21. Cutler R.L., Pielak G.J., Mauk A.G., Smith M. Replacement of cysteine-107 of Saccharomyces cerevisiae iso-1-cystochrome c with threonine: improved stability of the mutant protein. Protein Eng. 1:1987;95-99.
22. Amaki Y., Nakano H., Yamane T. Role of cysteine residues in esterase from Bacillus stearothermophilus and increasing its thermostability by the replacement of cysteines. Appl. Microbiol. Biotechnol. 40:1994;664-668.