Studies on osteogenic differentiation of rat bone marrow stromal cells cultured in type I collagen gel by RT-PCR analysis

Journal of Oral Rehabilitation

Volumn 30, Issue 8, 2003, Pages 802-807

  Taira, Masayuki ^a   Toyosawa, S ^b   Ijyuin, N ^b   Takahashi, J ^b   Araki, Y ^a  

^a IWATE MEDICAL UNIVERSITY   (Japan)

^b OSAKA UNIVERSITY   (Japan)

Author keywords

Bone marrow stromal cells; Collagen gel culture; Osteogenic differentiation; RT PCR; Tissue engineering

Indexed keywords

ALKALINE PHOSPHATASE; ASCORBIC ACID; COLLAGEN GEL; COLLAGEN TYPE 1; DEXAMETHASONE; GLYCEROL 2 PHOSPHATE; MESSENGER RNA; OSTEOCALCIN; OSTEONECTIN; OSTEOPONTIN; SIALOPROTEIN;

ANIMAL CELL; ARTICLE; BONE DEVELOPMENT; BONE MARROW CELL; CELL CULTURE; CELL DEATH; CELL DIFFERENTIATION; CONTROLLED STUDY; CULTURE MEDIUM; GENE EXPRESSION; MALE; NONHUMAN; PHENOTYPE; PROTEIN DETERMINATION; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STROMA CELL; TISSUE ENGINEERING;

ANIMALS; BONE MARROW CELLS; CELL DIFFERENTIATION; COLLAGEN TYPE I; CULTURE MEDIA; MALE; OSTEOGENESIS; RATS; RATS, SPRAGUE-DAWLEY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; STROMAL CELLS;

EID: 0141959782     PISSN: 0305182X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2842.2003.01170.x     Document Type: Article

References (17)

1. 3 on osteogenic differentiation of human marrow stromal cells in vitro. Archives of Oral Biology, 39, 941.
2. COELHO, M.J., TRIGO CABRAL, A. & FERNANDES, M.H. (2000a) Human bone cell cultures in biocompatibility testing. Part 1: Osteoblastic differentiation of serially passaged human bone marrow cells cultured in α-MEM and in DMEM. Biomaterials, 21, 1087.
3. COELHO, M.J. & FERNANDES, M.H. (2000b) Human Bone cell cultures in biocompatibility testing. Part II: Effect of ascorbic acid, β -glycerophosphate and dexamethasone on osteoblastic differentiation. Biomaterials, 21, 1095.
4. DECKERS, M.M., KARPERIEN, M., VAN DER BENT, C., YAMASHITA, T., PAPAPOULOS, S.E. & LOWIK, C.W. (2000) Expression of vascular endothelial growth factors and their receptors during osteoblast differentiation. Endocrinology, 141, 1667.
5. EFFRAH KAUFMANN, E.A., DUCHEYNE, P. & SHAPIRO, I.M. (2000) Evaluation of osteoblast response to porous bioactive glass (45S5) substrates by RT-PCR analysis. Tissue Engineering, 6, 19.
6. KINOSHITA, S., FINNEGAN, M., BUCHOLZ, R.W. & MIZUNO, K. (1999) Three-dimensional collagen gel culture promotes osteoblastic phenotype in bone marrow derived cells. Kobe Journal of Medical Science, 45, 201.
7. KINOSHITA, Y., KOBAYASHI, M., HIDAKA, T. & IKADA, Y. (1997) Reconstruction of mandibular continuity defects in dogs using poly (L-lactide) mesh and autogenic particulate cancellous bone and marrow: preliminary report. Journal of Oral & Maxillofacial Surgery, 55, 718.
8. KOMORI, T. (1998) Overview of Cbfa1/Pebp2αA in bone development. Experimental Medicine, 16, 1338 (in Japanese).
9. LIAN, J., STEWART, C., PUCHACZ, E., MACKOWIAK, S., SHALHOUB, V., COLLART, D., ZAMBETTI, C. & STEIN, G. (1989) Structure of the rat osteocalcin gene and regulation of vitamin D-dependent expression. Proceedings of the National Academy of Sciences of the United States of America, 86, 1143.
10. MASI, L., FRANCHI, A., SANTUCCI, M., DANIELLI, D., ARGANINI, L., GIANNONE, V., FORMIGLI, L., BENVENUTI, S., TANINI, A. & BEGHE, F. (1992) Adhesion, growth and matrix production by osteoblasts on collagen substrata. Calcified Tissue International, 51, 202.
11. NCBI (2000) Rat mRNA for Osteonectin, Complete Codes. Pubmed Nucleotide, Accession D28875, National Center of Biotechnology Information, National Library of Medicine of USA, USA.
12. OLDBERG, A., FRANZEN, A. & HEINEGARD, D. (1988) The primary structure of a cell-binding bone sialoprotein. The Journal of Biological Chemistry, 263, 19433.
13. SETO, I., ASAHINA, I., ODA, M. & ENOMOTO, S. (2001) Reconstruction of the primate mandible with a combination graft of recombinant human bone morphogenetic protein-2 and bone marrow. The Journal of Oral & Maxillofacial Surgery, 59, 53.
14. SINGH, K., MUKHERJEE, A.B., DE VOUGE, M.W. & MUKHER, J.B.B. (1992) Differential processing of osteopontin transcripts in rat kidney- and osteoblast-derived cell lines. The Journal of Biological Chemistry, 267, 23847.
15. THIEDE, M.A., YOON, K., GOLUB, E.E., NODA, M. & RODAN, G.A. (1988) Structure and expression of rat osteosarcoma (ROS 17/2.8) alkaline phosphatase: product of a single copy gene. Proceedings of the National Academy of Sciences of the United States of America, 85, 319.
16. YAO, K.L., TODESCAN, R. & SODEK, J. (1994) Temporal changes in matrix protein synthesis and mRNA expression during mineralized tissue formation by adult rat bone marrow cells in culture. Journal of Bone & Mineral Research, 9, 231.
17. YEH, J.K., EVANS, J.F., CHEN, M.M. & ALOIA, J.F. (1999) Effect of hypophysectomy on the proliferation and differentiation of rat bone marrow stromal cells. American Journal of Physiology, 276 (1 Pt 1), E34.