A method for multiple sequential analyses of macrophage functions using a small single cell sample

Brazilian Journal of Medical and Biological Research

Volumn 36, Issue 9, 2003, Pages 1221-1226

  Nascimento, F R F ^a   Rodriguez D ^a   Gomes, E ^a   Fernvik, E C ^a   Russo, M ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Hydrogen peroxide; Macrophages; Method; MHC II; Nitric oxide

Indexed keywords

BCG VACCINE; HYDROGEN PEROXIDE; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 2; NITRIC OXIDE; NITRIC OXIDE SYNTHASE; REACTIVE NITROGEN SPECIES; REACTIVE OXYGEN METABOLITE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE OXIDASE;

ANIMAL CELL; ANTIGEN EXPRESSION; ARTICLE; CELL ASSAY; CELL COUNT; CELL CULTURE; CONTROLLED STUDY; COST BENEFIT ANALYSIS; MACROPHAGE FUNCTION; METABOLITE; MOUSE; MYCOBACTERIUM; NONHUMAN; PERITONEUM CELL; PHENOTYPE; REPRODUCIBILITY; SEQUENTIAL ANALYSIS;

ACTINOBACTERIA (CLASS); ANIMALIA; MYCOBACTERIUM; MYCOBACTERIUM BOVIS BCG; UNCULTURED ACTINOMYCETE;

EID: 0141954922     PISSN: 0100879X     EISSN: 1414431X     Source Type: Journal    
DOI: 10.1590/S0100-879X2003000900012     Document Type: Article

References (15)

1. Bogdan C, Rollinghoff M & Diefenbach A (2000). Reactive oxygen and reactive nitrogen intermediates in innate and specific immunity. Current Opinion in Immunology, 12: 64-76.
2. Nathan C & Shiloh MU (2000). Reactive oxygen and nitrogen intermediates in the relationship between mammalian hosts and microbial pathogens. Proceedings of the National Academy of Sciences, USA, 97: 8841-8848.
3. Long EO (1989). Intracellular traffic and antigen processing. Immunology Today, 10: 232-234.
4. Pick E & Keisari Y (1980). A simple colorimetric method for the measurement of hydrogen peroxide produced by cells in culture. Journal of Immunological Methods, 38: 161-170.
5. Ding AH, Nathan CF & Stuehr DJ (1988). Release of reactive nitrogen intermediates and reactive oxygen intermediates from mouse peritoneal macrophages. Comparison of activating cytokines and evidence for independent production. Journal of Immunology, 141: 2407-2412.
6. Pick E & Mizel D (1981). Rapid microassays for the measurement of superoxide and hydrogen peroxide production by macrophages in culture using an automatic enzyme immunoassay reader. Journal of Immunological Methods, 46: 211-226.
7. Warren MK & Vogel SN (1985). Bone marrow-derived macrophages: development and regulation of differentiation markers by colony-stimulating factor and interferons. Journal of Immunology, 134: 982-989.
8. Pierres M, Kourilsky FM, Rebouah JP, Dosseto M & Caillol D (1980). Distinct epitopes of Ik gene products identified by monoclonal antibodies. European Journal of Immunology, 10: 950-957.
9. Nascimento FR, Calich VL, Rodriguez D & Russo M (2002). Dual role for nitric oxide in paracoccidioidomycosis: essential for resistance, but overproduction associated with susceptibility. Journal of Immunology, 168: 4593-4600.
10. Rabinovitch M, Manejias RE, Russo M & Abbey EE (1977). Increased spreading of macrophages from mice treated with interferon inducers. Cell Immunology, 29: 86-95.
11. DeChatelet LR, Shirley PS & Johnston Jr RB (1976). Effect of phorbol myristate acetate on the oxidative metabolism of human polymorphonuclear leukocytes. Blood, 47: 545-554.
12. Castagna M, Takai Y, Kaibuchi K, Sano K, Kikkawa U & Nishizuka Y (1982). Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters. Journal of Biological Chemistry, 257: 7847-7851.
13. Severn A, Wakelam MJ & Liew FY (1992). The role of protein kinase C in the induction of nitric oxide synthesis by murine macrophages. Biochemical and Biophysical Research Communications, 188: 997-1002.
14. Russo M, Teixeira HC, Marcondes MC & Barbuto JA (1989). Superoxide-independent hydrogen peroxide release by activated macrophages. Brazilian Journal of Medical and Biological Research, 22: 1271-1273.
15. Rodriguez D, Cavada BS, Abreu-de-Oliveira JT, de-Azevedo-Moreira R & Russo M (1992). Differences in macrophage stimulation and leukocyte accumulation in response to intraperitoneal administration of glucose/mannose-binding plant lectins. Brazilian Journal of Medical and Biological Research, 25: 823-826.