Use of human chondrocyte cell cultures to identify and characterize reactive antibodies in rheumatoid arthritis sera

Clinical and Experimental Rheumatology

Volumn 21, Issue 5, 2003, Pages 587-592

  Sabbatini, Alessandra ^b   Tacchetti, Carlo ^a   Tommasi, Simona ^b   Bongiorni, Francesca ^b   Migliorini, Paola ^b  

^a UNIVERSITY OF GENOA   (Italy)

^b UNIVERSITY OF PISA   (Italy)

Author keywords

Autoantibodies; Chondrocytes; Rheumatoid arthritis

Indexed keywords

AGAROSE; ANTIBODY; ANTIGEN; AUTOANTIBODY; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M;

ANTIBODY SCREENING; ANTIBODY SPECIFICITY; ANTIGEN DETECTION; ANTIGEN EXPRESSION; ANTIGEN SPECIFICITY; ARTICLE; CARTILAGE CELL; CELL CULTURE; CELL DIFFERENTIATION; CELL ISOLATION; CELL LYSATE; CELL MEMBRANE; CELL POPULATION; CELL SUSPENSION; CLINICAL ARTICLE; CONTROLLED STUDY; CULTURE MEDIUM; FIBROBLAST; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IMMUNOBLOTTING; IN VITRO STUDY; MONOLAYER CULTURE; PRIORITY JOURNAL; PROTEIN EXPRESSION; RHEUMATOID ARTHRITIS; SYNOVIAL FLUID;

ARTHRITIS, RHEUMATOID; AUTOANTIBODIES; AUTOANTIGENS; CELLS, CULTURED; CHONDROCYTES; FIBROBLASTS; FLOW CYTOMETRY; HUMANS; IMMUNOGLOBULIN G;

EID: 0141953813     PISSN: 0392856X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (25)

1. GAY S, GAY RE, KOOPMAN WJ: Molecular and cellular mechanisms of joint destruction in rheumatoid arthritis: two cellular mechanisms explain joint destruction ? Ann Rheum Dis 1993; 52: 839-47.
2. ALSALAMEH S, JAHN B, KRAUSE A, KALDEN JR, BURMESTER GR: Antigenicity and accessory cell function of human articular chondrocytes. J Rheumatol 1991; 18: 414-21.
3. YAMAGA KM, BOLEN H, KIMURA L, LANCE E: Enhanced chondrocyte destruction by lymphokine-activated killer cells. Arthritis Rheum 1993; 36: 500-13.
4. JAHN B, BURMESTER GR, SCHMID H, WESELOH G, ROHWER P, KALDEN JR: Changes in cell surface antigen expression on human articular chondrocytes induced by gamma-interferon. Arthritis Rheum 1987; 30: 64-74.
5. MOLLENHAUER J, VON DER MARK K, BURMESTER G, GLUCKERT K, LUTJEN-DRECOLL E, BRUME K: Serum antibodies against chondrocyte cell surface proteins in osteoarthritis and rheumatoid arthritis. J Rheumatol 1988; 15: 1811-7.
6. BANG H, MOLLENHAUER J, SCHULMEISTER A et al.: Isolation and characterization of a cartilage-specific membrane antigen (CH65): comparison with cytokeratins and heat-shock proteins. Immunology 1994; 81: 322-32.
7. TAKIGAWA M, TAJIMA K, PAN H, ENOMOTO M, KINOSHITA A, SUZUKI F: Establishment of a clonal human chondrosarcoma cell line with cartilage phenotypes. Cancer Res 1989; 49: 3996-4002.
8. HATTORI T, FUJISAWA T, SASAKI K et al.: Isolation and characterization of a rheumatoid arthritis-specific antigen (RA-A47) from a human chondrocytic cell line (HCS-2/8). Biochem Biophys Res Commun 1998; 245: 679-83.
9. CASTAGNOLA P, MORO G, DESCALZI-CANCEDDA F, CANCEDDA R: Type X collagen synthesis during in vitro development of chick embryo tibial chondrocytes. J Cell Biol 1986; 102: 2310-7.
10. AMBESI-IMPIOMBATO FS, PARKS LA, COON HG: Culture of hormone-dependent functional epithelial cells from rat thyroids. Proc Natl Acad Sci USA 1980; 77: 3455-9.
11. MILLER EJ, GAY S: Collagen: an overview. Meth Enzymol 1982; 82: 3-33
12. LAEMMLI EK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227: 680-5.
13. TOWBIN HT, STAEHELIN T, GORDON J: Electrophoretic transfer of proteins from polyacrilamide gels to nitrocellulose sheets. Proc Natl Acad Sci USA 1979; 76: 4350-5.
14. BLAKE MS, JOHNSTON KH, RUSSELL-JONES GJ, GOTSCHLICH EC: A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on western blots. Anal Biochem 1984; 136: 175-91.
15. TACCHETTI C, QUARTO R, NITSCH L, HARTMANN DJ, CANCEDDA R: In vitro morphogenesis of chick embryo hypertrophic cartilage. J Cell Biol 1987; 105: 999-1006.
16. KOSHER RA: The chondroblast and the chondrocyte. In HALL BK (Ed.): Cartilage, Oxford, Academic Press Inc. 1983, 1: 59-85.
17. HAYASHI M, NINOMIYA Y, PARSON J, HAYASHI K, OLSEN BR, TRELSTAD RL: Differential localization of mRNA of collagen type I and II in chick fibroblast, chondrocytes and corneal cells by in situ hybridization using cDNA probes. J Cell Biol 1986; 102: 2302-9.
18. VON DER MARK H, VON DER MARK K, GAY S: Study of differential collagen synthesis during development of the chick embryo by immunofluorescence. I. Preparation of collagen type I and type II antibodies and their application to early stages of the chick embryo. Dev Biol 1976; 48: 237-49.
19. BENYA PD, SHAFFER JD: Dedifferentiated chondrocytes re-express the differentiated collagen phenotype when cultured in agarose gels. Cell 1982; 30: 215-24.
20. BRITTBERG M, LINDAHL A, NILSSON A, OHLSSON C, ISAKSSON O, PETERSON L: Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation. N Engl J Med 1994; 331: 799-895.
21. ALSALAMEH S, MOLLENHAUER J, HAIN N, STOCKS K, KALDEN JR, BURMESTER GR: Cellular immune response toward human articular chondrocytes. Arthritis Rheum 1990; 33: 1477-86.
22. SEKINE T, MASUKO-HONGO K, MATSUI T, ASAHARA H, TAKIGAWA M, NISHIOKA K, KATO T: Recognition of YKL-39, a human cartilage related protein, as a target antigen in patients with rheumatoid arthritis. Ann Rheum Dis 2001; 60: 49-54
23. SAKATA M, MASUKO-HONGO K, TSURUHA J, SEKINE T, NAKAMURA H, TAKIGAWA M, NISHIOKA K, KATO T: YKL-39, a human cartilage-related protein, induces arthritis in mice. Clin Exp Rheumatol 2002; 20: 343-50.
24. TSURUHA J, MASUKO-HONGO K, KATO T, SAKATA M, NAKAMURA H, SEKINE T, TAKIGAWA M, NISHIOKA K: Autoimmunity against YKL-39, a human cartilage derived protein, in patients with osteoarthritis. J Rheumatol 2002; 29: 1459-66.
25. TAKAGI T, JASIN HE: Interactions of synovial fluid immunoglobulins with chondrocytes. Arthritis Rheum 1992; 35: 1502-9.