Molecular cloning and expression of guinea pig cytochrome P450c21 cDNA (steroid 21-hydroxylase) isolated from the adrenals

Journal of Steroid Biochemistry and Molecular Biology

Volumn 86, Issue 2, 2003, Pages 123-132

  Martineau, Isabelle ^a   Bélanger, Alain ^a   Tchernof, André ^a   Tremblay, Yves ^a  

^a UNIVERSITÉ LAVAL   (Canada)

Author keywords

17 Hydroxyprogesterone; 21 Hydroxylase; Adrenals; Glucocorticoids; Guinea pig; P450c21; Progesterone

Indexed keywords

AMINO ACID; COMPLEMENTARY DNA; CORTICOTROPIN; CORTODOXONE; CYCLIC AMP; DEOXYCORTICOSTERONE; ESTROGEN; GLUCOCORTICOID; HYDROXYPROGESTERONE; MINERALOCORTICOID; PROGESTERONE; RECOMBINANT PROTEIN; STEROID 21 MONOOXYGENASE;

ADRENAL GLAND; AMINO ACID SEQUENCE; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DNA HYBRIDIZATION; DNA ISOLATION; DNA TRANSCRIPTION; ENZYME ACTIVITY; ENZYME ISOLATION; ESTROGEN ACTIVITY; GENETIC CONSERVATION; GUINEA PIG; HUMAN; HUMAN CELL; MOLECULAR CLONING; MOLECULAR DYNAMICS; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN PROCESSING; RAT; SPECIES COMPARISON; STEROID HYDROXYLATION; TISSUE DISTRIBUTION;

CAVIA PORCELLUS; MAMMALIA; SUS SCROFA;

EID: 0141928685     PISSN: 09600760     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-0760(03)00261-9     Document Type: Article

References (43)

1. Miller W.L. Molecular biology of steroid hormone synthesis. Endocr. Rev. 9(3):1988;295-318.
2. Aisenberg J.E., Speiser P.W. The genetics of 21-hydroxylase deficiency. Endocrinologist. 4(2):1994;92-98.
3. Chaplin D.D., Galbraith L.J., Seidman J.G., White P.C., Parker K.L. Nucleotide sequence analysis of murine 21-hydroxylase genes: mutations affecting gene expression. Proc. Natl. Acad. Sci. U.S.A. 83(24):1986;9601-9605.
4. Higashi Y., Yoshioka H., Yamane M., Gotoh O., Fujii-Kuriyama Y. Complete nucleotide sequence of two steroid 21-hydroxylase genes tandemly arranged in human chromosome: a pseudogene and a genuine gene. Proc. Natl. Acad. Sci. U.S.A. 83(9):1986;2841-2845.
5. Morel Y., Bristow J., Gitelman S.E., Miller W.L. Transcript encoded on the opposite strand of the human steroid 21-hydroxylase/complement component C4 gene locus. Proc. Natl. Acad. Sci. U.S.A. 86(17):1989;6582-6586.
6. Miller J.W., Gitelman S.E., Bristow J., Morel Y. Analysis of the duplicated human C4/P450c21/X gene cluster. J. Steroid Biochem. Mol. Biol. 48(8):1992;691-697.
7. Bristow J., Gitelman S.E., Tee M.K., Staels B., Miller W.L. Abundant adrenal-specific transcription of the human P450c21A "pseudogene" J. Biol. Chem. 268(17):1993;12919-12924.
8. Gitelman S.E., Bristow J., Miller W.L. Mechanism and consequences of the duplication of the human C4/P450c21/gene X locus. Mol. Cell. Biol. 12(7):1992;3313-3314.
9. Chung B.C., Matteson K.J., Miller W.L. Cloning and characterization of the bovine gene for steroid 21-hydroxylase (P-450c21). DNA. 4(3):1985;211-219.
10. Yoshioka H., Morohashi K., Sogawa K., Yamane M., Kominami S., Takemori S., Okada Y., Omura T., Fujii-Kuriyama Y. Structural analysis of cloned cDNA for mRNA of microsomal cytochrome P-450(C21) which catalyzes steroid 21-hydroxylation in bovine adrenal cortex. J. Biol. Chem. 261(9):1986;4106-4109.
11. Chung B.C., Matteson K.J., Miller W.L. Structure of a bovine gene for P-450c21 (steroid 21-hydroxylase) defines a novel cytochrome P-450 gene family. Proc. Natl. Acad. Sci. U.S.A. 83(12):1986;4243-4247.
12. Helmberg A. Twin genes and endocrine disease: CYP21 and CYP11B genes. Acta Endocrinol. (Copenh). 129(2):1993;97-108.
13. Chang S.F., Chung B.C. Difference in transcriptional activity of two homologous CYP21A genes. Mol. Endocrinol. 9(10):1995;1330-1336.
14. Tusie-Luna M.T., White P.C. Gene conversions and unequal crossovers between CYP21 (steroid 21-hydroxylase gene) and CYP21P involve different mechanisms. Proc. Natl. Acad. Sci. U.S.A. 92(23):1995;10796-10800.
15. Provencher P.H., Tremblay Y., Belanger A. Effects of C19 steroids on adrenal steroidogenic enzyme activities and their mRNA levels in guinea-pig fasciculata-glomerulosa cells in primary culture. J. Endocrinol. 132(2):1992;269-276.
16. Provencher P.H., Tremblay Y., Fiet J., Belanger A. Effect of ACTH on steroidogenic enzymes in guinea pig fasciculata-glomerulosa cells: changes in activity and mRNA levels. J. Steroid Biochem. Mol. Biol. 41(1):1992;59-67.
17. Provencher P.H., Tremblay Y., Caron S., Belanger A. Effect of chronic ACTH treatment on guinea-pig adrenal steroidogenesis: steroid plasma levels, steroid adrenal levels, activity of steroidogenic enzymes and their steady-state mRNA levels. J. Steroid. Biochem. Mol. Biol. 41(1):1992;69-78.
18. Matteson K.J., Picado-Leonard J., Chung B.C., Mohandas T.K., Miller W.L. Assignment of the gene for adrenal P450c17 (steroid 17α-hydroxylase/17,20 lyase) to human chromosome 10. J. Clin. Endocrinol. Metab. 63(3):1986;789-791.
19. Chirgwin J.M., Przybyla A.E., Macdonald R.J., Rutter W.J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 18(24):1979;5294-5299.
20. Tremblay Y., Fleury A., Beaudoin C., Vallee M., Belanger A. Molecular cloning and expression of guinea pig cytochrome P450c17 cDNA (steroid 17 alpha-hydroxylase/17,20 lyase): tissue distribution, regulation and substrate specificity of the expressed enzyme. DNA Cell. Biol. 13(12):1994;1199-1212.
21. Tremblay Y., Ringler G.E., Morel Y., Mohandas T.K., Labrie F., Strauss III J.F., Miller W.L. Regulation of the gene for estrogenic 17-ketosteroid reductase lying on chromosome 17cen - q25. J. Biol. Chem. 264(34):1989; 20458-20462.
22. Clements J.A., Matheson B.A., Wines D.R., Brady J.M., Macdonald R.J., Funder J.W. Androgen dependence of specific kallikrein gene family members expressed in rat prostate. J. Biol. Chem. 263(31):1988;16132-16137.
23. Tremblay Y., Beaudoin C. Regulation of 3 β-hydroxysteroid dehydrogenase and 17 beta-hydroxysteroid dehydrogenase messenger ribonucleic acid levels by cyclic adenosine 3′,5′-monophosphate and phorbol myristate acetate in human choriocarcinoma cells. Mol. Endocrinol. 7(3):1993;355-364.
24. Provencher P., Lorrain A., Belanger A., Fiet J. Steroid biosynthesis by zona glomerulosa-fasciculata cells in primary culture of guinea-pig adrenals. J. Steroid Biochem. 36(6):1990;589-596.
25. K. Takada, J. Kitamura, K. Kanehira, M. Takiguti, A. Hashimoto, Canine P450c21 (21-hydroxylase) mRNA, GenBank, 2000.
26. White P.C., New M.I., Dupont B. Cloning and expression of cDNA encoding a bovine adrenal cytochrome P-450 specific for steroid 21-hydroxylation. Proc. Natl. Acad. Sci. U.S.A. 81(7):1984;1986-1990.
27. Burghelle-Mayeur C., Geffrotin C., Vaiman M. Sequences of the swine 21-hydroxylase gene (CYP21) and a portion of the opposite-strand overlapping gene of unknown function previously described in human. Biochim. Biophys. Acta. 1171(2):1992;153-161.
28. Crawford R.J., Hammond V.E., Connell J.M., Coghlan J.P. The structure and activity of two cytochrome P450c21 proteins encoded in the ovine adrenal cortex. J. Biol. Chem. 267(23):1992;16212-16218.
29. Zhou Z., Agarwal V.R., Dixit N., White P., Speiser P.W. Steroid 21-hydroxylase expression and activity in human lymphocytes. Mol. Cell. Endocrinol. 127(1):1997;11-18.
30. Lajic S., Eidsmo L., Holst M. Steroid 21-hydroxylase in the kidney: demonstration of levels of messenger RNA which correlate with the level of activity. J. Steroid Biochem. Mol. Biol. 52(2):1995;181-186.
31. Endoh A., Natsume H., Igarashi Y. Dual regulation of 21-hydroxylase activity by sex steroid hormones in rat hepatocytes. J. Steroid Biochem. Mol. Biol. 54(3-4):1995;163-165.
32. Ramarao M., Kemper B. Substitution at residue 473 confers progesterone 21-hydroxylase activity to cytochrome P450 2C2. Mol. Pharmacol. 48(3):1995;417-424.
33. Narasimhulu S. Heterogeneity of the bovine adrenal steroid 21-hydroxylase. Endocrinol. Res. 15(1-2):1989;67-84.
34. Colby H.D., Levitt M., Pope M.R., Johnson P.B. Differential effects of adrenocorticotropic hormone on steroid hydroxylase activities in the inner and outer zones of the guinea pig adrenal cortex. J. Steroid Biochem. Mol. Biol. 42(3-4):1992;329-335.
35. Vallee M., Perron S., Tremblay Y., Belanger A. Regulation of guinea pig adrenal P450c21 messenger RNA, protein and activity by RU486. J. Steroid Biochem. Mol. Biol. 54(1-2):1995;31-38.
36. Hanukoglu I., Feuchtwanger R., Hanukoglu A. Mechanism of corticotropin and cAMP induction of mitochondrial cytochrome P450 system enzymes in adrenal cortex cells. J. Biol. Chem. 265(33):1990;20602-20608.
37. Chang C.W., Naseeruddin S.A., Hornsby P.J. The response of 21-hydroxylase messenger ribonucleic acid levels to adenosine 3′,5′-monophosphate and 12-O-tetradecanoylphorbol-13-acetate in bovine adrenocortical cells is dependent on culture conditions. Endocrinology. 128(1):1991;604-610.
38. Imai T., Seo H., Murata Y., Ohno M., Satoh Y., Funahashi H., Takagi H., Matsui N. Alteration in the expression of genes for cholesterol side-chain cleavage enzyme and 21-hydroxylase by hypophysectomy and ACTH administration in the rat adrenal. J. Mol. Endocrinol. 4(3):1990;239-245.
39. Parker K.L., Chaplin D.D., Wong M., Seidman J.G., Smith J.A., Schimmer B.P. Expression of murine 21-hydroxylase in mouse adrenal glands and in transfected Y1 adrenocortical tumor cells. Proc. Natl. Acad. Sci. U.S.A. 82(23):1985;7860-7864.
40. Crawford P.A., Sadovsky Y., Woodson K., Lee S.L., Milbrandt J. Adrenocortical function and regulation of the steroid 21-hydroxylase gene in NGFI-B-deficient mice. Mol. Cell. Biol. 15(8):1995;4316-4331.
41. Xu D., Voigt J.M., Mico B.A., Colby H.D. Inhibition of adrenal steroid metabolism by administration of 1-aminobenzotriazole to guinea pigs. J. Steroid Biochem. Mol. Biol. 54(5-6):1995;281-285.
42. Goldman D., Yawetz A. The interference of polychlorinated biphenyls (Aroclor 1254) with membrane regulation of the activities of cytochromes P-450C21 and P-450(17) α-lyase in guinea-pig adrenal microsomes. J. Steroid Biochem. Mol. Biol. 42(1):1992;37-47.
43. Belanger A., Tremblay Y., Vallee M., Provencher P.H., Perron S. Regulation of 21-hydroxylase activity by steroids. Endocrinol. Res. 21(1-2):1995;329-341.