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Volumn 75, Issue 19, 2003, Pages 5116-5123

High-throughput nuclear magnetic resonance analysis using a multiple coil flow probe

Author keywords

[No Author keywords available]

Indexed keywords

ELECTRIC EXCITATION; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PROBES; ROBOTICS;

EID: 0141924426     PISSN: 00032700     EISSN: None     Source Type: Journal    
DOI: 10.1021/ac034400r     Document Type: Article
Times cited : (58)

References (42)
  • 18
    • 0141952568 scopus 로고    scopus 로고
    • http://www.bruker-biospin.de/NMR/best/best3.html, as of June 23, 2003.
  • 19
    • 0141952569 scopus 로고    scopus 로고
    • http://www.varianinc.com/cgi-bin/nav?varinc/docs/products/nmr/accessory/ auto_samplers/vast/index&cid=975JINOMIIRQLGLPQNQRJGJ, as of June 23, 2003.
  • 36
    • 0141987209 scopus 로고    scopus 로고
    • note
    • Before any experiments could be run, all four lines had to be calibrated with respect to one another. All lines were calibrated by pushing 500, μL of water through the empty lines at a flow rate of 0.48 mL/min while acquiring spectra every 0.125 s corresponding to a spectrum every 1 μL. The spectrum with the first evidence of the water peak for each line was recorded and compared for all lines. The experiment was run 7 times for each line, and the lines were cut until the spectral appearances which occurred were within one unit (1 μL) of each other. The standard deviation of 7 runs was 2 μL units.
  • 37
    • 0141883644 scopus 로고    scopus 로고
    • note
    • 2O was used as the sample because it has one, singlet peak from which the line width at half-height is easily measured. The line width of the glycine peak was used to gauge whether the sample was centered around the coil. If the sample was not centered and the air bubble on either end of the sample was within 0.5 cm of the coil, then the line width would broaden from its normal value of 1-2 Hz until the peak disappeared into the baseline. With a 25 μL sample, it was found that the push volume could fall within an 8 μL window, and the glycine line width would still be < 2 Hz for each coil. The windows for each line overlapped in the range of 121-128 μL. Thus, 125 μL of push solvent was used to center the samples.
  • 39
    • 0141952567 scopus 로고    scopus 로고
    • note
    • The Gaussian pulse width, transmitter power, and gradient strengths were determined. The pulse width and transmitter power were chosen to give a 90 degree pulse with a 1350 Hz excitation width at half-height and 8200 Hz at 1% height. The gradient was chosen to separate the centers of the spectral windows of neighboring coils by 9000 Hz. The reverse gradient strength was chosen to give the best S/N for each coil.
  • 40
    • 0141918179 scopus 로고    scopus 로고
    • note
    • Currently, when the shim values are arrayed, an internal delay between scans results with the Varian INOVA spectrometer. The delay is 4-6 s/scan. When this problem is circumvented, the number of scans that can be performed for each sample in the 69 s window of analysis time can be increased from 1 to 5 without adding time to the automation method.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.