Purification and partial characterization of a lectin from Caesalpinia tinctoria Domb, ex Dc fruits

Brazilian Journal of Plant Physiology

Volumn 15, Issue 2, 2003, Pages 119-122

  De Oliveira, Marli Lourdes ^a   Beltramini, Leila Maria ^e   De Simone, Salvatore Giovanni ^b   Brumano, Maria Helena Nasser ^a   Silva Lucca, Rosemeire Aparecida ^c   Nakaema, Marcelo Kiyoshi Kian ^d   Pires, Christiano Vieira ^a   De Almeida Oliveira, Maria Goreti ^a  

^a FEDERAL UNIVERSITY OF VIÇOSA   (Brazil)

^b INSTITUTO OSWALDO CRUZ   (Brazil)

^c Ctro Engenharias Cie E   (Brazil)

^d UNIVERSITY OF CAMPINAS   (Brazil)

^e NONE

Author keywords

Circular dichroism; Leguminosae; Plant defense; Pod; Secondary structure

Indexed keywords

CAESALPINIA; CAESALPINIA TINCTORIA; FABACEAE;

EID: 0141889949     PISSN: 16770420     EISSN: None     Source Type: Journal    
DOI: 10.1590/s1677-04202003000200008     Document Type: Article

References (14)

1. Alonzo N, Hirs CHW (1968) Automation of sample application in amino acid analyzers. Anal. Biochem. 23:272-288.
2. Bradford MM(1976) A rapid and sensitive method for the quantitation of microgram protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
3. Cantor CR, Schimmel PR (1980) Part II: Techniques for the study of biological structure and function: Spectroscopic Analysis of Biopolymers. Biophysical Chemistry, pp. 846-910. WH Freeman and Company, New York.
4. Carlini CR, Grossi-de-Sá MF (2002) Plant toxic proteins with insecticidal properties. A review on their potentialities as bioinsecticides. Toxicon 40:1515-1539.
5. Cohen SA, Strydom DJ (1988) Amino acid analysis utilizing phenylisothiocyanate derivatives. Anal. Biochem. 174:1-16.
6. De Simone SG, Santos R, Araujo MF, Pinho RT (1994) Preparative isolation of the lectin jacalin by anion-exchange high-performance liquid chromatography. J. Chromatogr. 688:357-362.
7. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal. Chem. 28:350-356.
8. Galvez JMG, Rieddl B, Conner AH (1997) Analytical studies on tara tannins. Holzforschung 51:235-243.
9. Goldstein IJ, Poretz RD (1986) Isolation, physicochemical characterization, and carbohydrate-binding specificity of lectins. In: Liener, IE, Sharon, N, Goldstein, IJ (eds), The Lectins: properties, functions, and applications in biology and medicine, pp.33-247. Academic Press, Orlando.
10. Loris R, Hamelryck T, Bouckaert J, Wyns L (1998) Legume lectin structure. Biochim. Biophys. Acta 1383:9-36.
11. Peumans WJ, de Ley M, Stinissen HM, Broekaert WF (1985) Isolation and partial characteriztion of a new lectin from seeds of the greater celandine (Chelidonium majus). Plant Physiol. 78:379-383.
12. Peumans WJ, Van Damme EJM (1995) Lectin as plant defense proteins. Plant Physiol. 109:347-352.
13. Sreerama N, Woody RW (1994) Poly(Pro)II helix in globular proteins: Identification and circular dichroic analysis. Biochemistry 33:10022-10025.
14. Toms GC, Western A (1971) Phytohaemagglutinins. In: Harbone, JB, Boulter, D, Turner, BL (eds), Chemotaxonomy of the Leguminosae, pp.367-462. Academic Press Inc, London.