Multiplex PCR for simultaneous detection of enterococcal genes vanA and vanB and staphylococcal genes mecA, ileS-2 and femB

International Microbiology

Volumn 6, Issue 2, 2003, Pages 113-115

  Ramos Trujillo, Elena ^a   Pérez Roth, Eduardo ^a   Méndez Alvarez, Sebastián ^a,b   Claverie Martín, Félix ^a  

^a HOSPITAL UNIVERSITARIO NUESTRA SEÑORA DE CANDELARIA   (Spain)

^b UNIVERSIDAD DE LA LAGUNA   (Spain)

Author keywords

Antibiotic resistance; Glycopeptides; Methicillin resistant S. aureus; Staphylococcus aureus; Vancomycin resistant S. aureus

Indexed keywords

METICILLIN; PSEUDOMONIC ACID; VANCOMYCIN;

ARTICLE; BACTERIAL GENE; BACTERIAL GENE ILES 2; BACTERIAL GENE MECA; BACTERIAL GENE VANA; BACTERIAL GENE VANB; BACTERIAL STRAIN; BACTERIUM COLONY; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; CONTROLLED STUDY; DNA DETERMINATION; ENTEROCOCCUS; GENE IDENTIFICATION; NONHUMAN; POLYMERASE CHAIN REACTION; PRACTICE GUIDELINE; STAPHYLOCOCCUS;

BACTERIA (MICROORGANISMS); ENTEROCOCCUS; ENTEROCOCCUS FAECALIS; STAPHYLOCOCCUS; STAPHYLOCOCCUS AUREUS;

EID: 0141889261     PISSN: 11396709     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10123-003-0118-z     Document Type: Article

References (24)

1. Anthony RM, Connor AM, Power EGM, French GL (1999) Use of the polymerase chain reaction for rapid detection of high-level mupirocin resistance in staphylococci. Eur J Clin Microbiol Infect Dis 18:30-34
2. Archer GL (1998) Staphylococcus aureus: a well-armed pathogen. Clin Infect Dis 26:1179-1181
3. Avison MB, Bennett PM, Howe RA, Walsh TR (2002) Preliminary analysis of the genetic basis for vancomycin resistance in Staphylococcus aureus strain Mu50. J Antimicrob Chemother 49:255-260
4. Cetinkaya Y, Falk P, Mayhall CG (2000) Vancomycin-resistant enterococci. Clin Microbiol Rev 13:686-707
5. Chambers HF (1997) Methicillin resistance in staphylococci: molecular and biochemical basis and clinical implications. Clin Microbiol Rev 10:781-791
6. Chambers HF (2001) The changing epidemiology of Staphylococcus aureus? Emerg Infect Dis 7:178-182
7. Dutka-Malen S, Evers S, Courvalin P (1995) Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR. J Clin Microbiol 33:24-27
8. Geha DJ, Uhl JR, Gustaferro CA, Persing DH (1994) Multiplex PCR for identification of methicillin-resistant staphylococci in the clinical laboratory. J Clin Microbiol 32:1768-1772
9. Hamilton-Miller JM (2002) Vancomycin-resistant Staphylococcus aureus: a real and present danger? Infection 30:118-124
10. Hiramatsu K, Hanaki H, Ino T, Yabuta K, Oguri T, Tenover FC (1997) Methicillin-resistant Staphylococcus aureus clinical strain with reduced vancomycin susceptibility. J Antimicrob Chemother 40:135-136
11. Kobayashi N, Wu H, Kojima K, Taniguchi K, Urasawa S, Uehara N, Omizu Y, Kishi Y, Yagihashi A, Kurokawa I (1994) Detection of mecA, femA and femB genes in clinical strains of staphylococci using polymerase chain reaction. Epidemiol Infect 113:259-266
12. Ligozzi M, Lo Cascio G, Fontana R (1998) VanA gene cluster in a vancomycin-resistant clinical isolate of Bacillus circulans. Antimicrob Agents Chemother 42:2055-2059
13. Méndez-Álvarez S, Pérez-Hernández X, Claverie-Martín F (2000) Glycopeptide resistance in enterococci. Int Microbiol 3:71-80
14. Miller D, Urdaneta V, Weltman A (2002) Public health dispatch: Vancomycin-resistant Staphylococcus aureus - Pennsylvania, 2002. Morb Mortal Wkly Rep 2002 51:902
15. Murray BE (2000) Vancomycin-resistant enterococcal infections. N Engl J Med 342:710-721
16. Noble WC, Virani Z, Cree RGA (1992) Co-transfer of vancomycin and other resistance genes from Enterococcus faecalis NCTC 12201 to Staphylococcus aureus. FEMS Microbiol Lett 93:195-198
17. Pérez-Hernández X, Méndez-Álvarez S, Claverie-Martín F (2002) A PCR assay for rapid detection of vancomycin resistant enterococci. Diag Microbiol Infect Dis 42:273-277
18. Pérez-Roth E, Claverie-Martín F, Villar J, Méndez-Álvarez S (2001) Multiplex PCR for simultaneous identification of Staphylococcus aureus and detection of methicillin and mupirocin resistance. J Clin Microbiol 39:4037-4041
19. Pérez-Roth E, Claverie-Martín F, Batista N, Moreno A, Méndez-Álvarez S (2002) Mupirocin resistance in methicillin-resistant Staphylococcus aureus clinical isolates in a Spanish hospital. Co-application of multiplex PCR assay and conventional microbiology methods. Diag Microbiol Infect Dis 43:123-128
20. Poyart C, Pierre C, Quesne G, Pron B, Berche P, Trieu-Cuot P (1997) Emergence of vancomycin resistance in the genus Streptococcus: characterization of a vanB transferable determinant in Streptococcus bovis. Antimicrob Agents Chemother 41:24-29
21. Rice LB (2001) Emergence of vancomycin-resistant enterococci. Emerg Infect Dis 7:183-187
22. Sieradzki K, Pinho MG, Tomasz A (1999) Inactivated pbp4 in highly glycopeptide-resistant laboratory mutants of Staphylococcus aureus. J Biol Chem 274:18942-18946
23. Sievert DM, Boulton ML, Stoltman G, Johnson D, Stobierski MG, Downes FP, Somsel PA, Rudrik JT, Brown W, Hafeez W, Lundstrom T, Flanagan E, Johnson R, Mitchell J, Chang S (2002) Staphylococcus aureus resistant to vancomycin - United States, 2002. Morb Mortal Wkly Rep 51:565-567
24. Srinivasan A, Dick JD, Perl TM (2002) Vancomycin resistance in staphylococci. Clin Microbiol Rev 15:430-438