Electrostatic sensor for identifying interactions between peptides and bacterial membranes

Molecular Immunology

Volumn 40, Issue 7, 2003, Pages 407-411

  Fitchen, Nicola ^a,c   O'Shea, Paul ^b   Williams, Paul ^a   Hardie, Kim R ^a  

^a UNIVERSITY OF NOTTINGHAM   (United Kingdom)

^b QUEEN S MEDICAL CENTRE   (United Kingdom)

^c Units 3 and 4   (United Kingdom)

Author keywords

H. pylori; Membrane interactions; Peptide; VacA

Indexed keywords

FLUORESCEIN; PHOSPHATIDYLETHANOLAMINE; SYNTHETIC PEPTIDE; XANTHENE;

ARTICLE; BACTERIAL CELL; BINDING AFFINITY; BIOTECHNOLOGY; CELL MEMBRANE; ELECTRIC POTENTIAL; IMMUNOFLUORESCENCE; IMMUNOSENSOR; MEMBRANE BINDING; MOLECULAR INTERACTION; NONHUMAN; PRIORITY JOURNAL; PROKARYOTE; PROTEIN PROTEIN INTERACTION; PROTON TRANSPORT; SPECTROPHOTOMETRY;

BACTERIA (MICROORGANISMS); EUKARYOTA; INSERTION SEQUENCES; PROKARYOTA;

EID: 0141884337     PISSN: 01615890     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0161-5890(03)00153-6     Document Type: Article

References (29)

1. Asawakarn T., Cladera J., O'Shea P. Effects of the membrane dipole potential on the interaction of saquinavir with phospholipid membranes and plasma membrane receptors of Caco-2 cells. J. Biol. Chem. 276:2001;38457-38463.
2. Cladera, J., O'Shea, P., 2001. Generic techniques for fluorescence measurements of protein-ligand interactions real-time kinetics and spatial imaging. In: Harding, Chowdery (Eds.), Protein-Ligand Interactions. Oxford University Press, Oxford, UK, pp. 169-200.
3. Cover T.L. The vacuolating cytotoxin of Helicobacter pylori. Mol. Microbiol. 20:1996;241-246.
4. Cover T.L., Blaser M.J. Purification and characterization of the vacuolating toxin from Helicobacter pylori. J. Biol. Chem. 267:1992;10570-10575.
5. Cover T.L., Tummuru M.K., Cao P., Thompson S.A., Blaser M.J. Divergence of genetic sequences for the vacuolating cytotoxin among Helicobacter pylori strains. J. Biol. Chem. 269:1994;10566-10573.
6. Cover T.L., Hanson P.I., Heuser J.E. Acid induced dissociation of VacA, the Heliocbacter pylori vacuolating cytotoxin, reveals its pattern of assembly. J. Cell Biol. 138:1997;759-769.
7. Ernst P.B., Gold B.D. The disease spectrum of Helicobacter pylori: the immunopathogenesis of gastric ulcer and gastric cancer. Annu. Rev. Microbiol. 54:2000;615-640.
8. Galmiche A., Rassow J., Doye A., Cagnol S., Chambard J.-C., Contamin S., de Thillot V., Just I., Ricci V., Solcia E., Van Obberghen E., Boquet P. The N-terminal 34 kDa fragment of Helicobacter pylori vacuolating cytotoxin targets mitochondria and induces cytochrome c release. EMBO J. 19:2000;6361-6370.
9. Garner J.A., Cover T.L. Binding and internalization of the Helicobacter pylori vacuolating cytotoxin by epithelial cells. Infect. Immun. 64(10):1996;4197-4203.
10. Henderson I.R., Navarro-Garcia F., Nataro J.P. The great escape: structure and function of the autotransporter proteins. Trends Microbiol. 6:1998;370-378.
11. Henderson I.R., Cappello R., Nataro J.P. Autotransporter proteins, evolution and redefining protein secretion. Trends Microbiol. 8:2000;529-532.
12. Iwamoto H., Czajkowsky D.M., Cover T.L., Szabo G., Shao Z. VacA from Helicobacter pylori: a hexameric chloride channel. FEBS Lett. 450:1999;101-104.
13. Kimura M., Goto S., Wada A., Yahiro K., Niidome T., Hatakeyama T., Aoyagi H., Hirayama T., Kondo T. Vacuolating cytotoxin purified from Helicobacter pylori causes mitochondrial damage in human gastric cells. Microb. Pathog. 26:1999;45-52.
14. Kuck D., Kolmerer B., Iking-Konert C., Krammer P.H., Rudi J. Vacuolating cytotoxin of Helicobacter pylori induces apoptosis in the human gastric epithelial cell line AGS. Infect. Immun. 69:2001;5080-5087.
15. Lupetti P., Heuser J.E., Manetti R., Massari P., Lanzavecchia S., Bellon P.L., Dallai R., Rappuoli R., Telford J.L. Oligomeric and subunit structure of the Helicobacter pylori vacuolating cytotoxin. J. Cell Biol. 133:1996;801-807.
16. Molinari M., Galli C., Norais N., Telford J., Rappuoli R., Luzio J.P., Montecucco C. Vacuoles induced by Helicobacter pylori toxin contain both late endosomal and lysosomal markers. J. Biol. Chem. 272:1997;25339-25344.
17. Molinari M., Salio M., Galli C., Norais N., Rappuoli R., Lanzavecchia A., Montecucco C. Selective inhibition of Li-dependent antigen presentation by Helicobacter pylori toxin VacA. J. Exp. Med. 187:1998a;135-140.
18. Molinari M., Galli C., de Bernard M., Norais N., Ruysschaert J.-M., Rappuoli R., Montecucco C. The acid activation of Helicobacter pylori toxin VacA: structural and membrane binding studies. Biochem. Biophys. Res. Commun. 248:1998b;334-340.
19. Moll G., Papini E., Colonna R., Burroni D., Telford J., Rappuoli R., Montecucco C. Lipid interaction of the 37-kDa and 58-kDa fragments of the Helicobacter pylori cytotoxin. Eur. J. Biochem. 234:1995;947-952.
20. O'Toole P.J., Morrison I.E.G., Cherry R.J. Investigations of spectrin-lipid interactions using fluoresceinphosphatidylethanolamine as a membrane probe. Biochim. Biophys. Acta. 1466:2000;39-46.
21. Papini E., Satin B., Norais N., de Bernard M., Telford J.L., Rappuoli R., Montecucco C. Helicobacter pylori vacuolating cytotoxin increases the permeability of polarized epithelial cell monolayers. J. Clin. Invest. 102:1998;813-820.
22. Pelicic V., Reyrat J.-M., Sartori L., Pagliaccia C., Rappuoli R., Telford J.L., Montecucco C., Papini E. Helicobacter pylori VacA cytotoxin associated with the bacteria increases epithelial permeability independently of its vacuolating activity. Microbiology. 145:1999;2043-2050.
23. Schmitt W., Haas R. Genetic analysis of the Helicobacter pylori vacuolating cytotoxin: structural similarities with the IgA protease type of exported protein. Mol. Microbiol. 12:1994;307-319.
24. Sijbrandi R., Urbanus M.L., ten Hagen-Jongman C.M., Bernstein H.D., Ougega B., Otto B.R., Luirink J. Signal Recognition Particle (SRP)-mediated targeting and Sec-dependent translocation of an extracellular Escherichia coli protein. J. Biol. Chem. 278(7):2003;4654-4659.
25. Szabò I., Brutsche S., Tombola F., Moschioni M., Satin B., Telford J.L., Rappuoli R., Montecucco C., Papini E., Zoratti M. Formation of anion-selective channels in the cell plasma membrane by the toxin VacA of Helicobacter pylori is required for its biological activity. EMBO J. 18:1999;5517-5527.
26. Telford J.L., Ghiara P., Dellorco M., Comanducci M., Burroni D., Bugnoli M., Tecce M.F., Censini S., Covacci A., Xiang Z.Y., Papini E., Montecucco C., Parente L., Rappuoli R. Gene structure of the Helicobacter pylori cytotoxin and evidence of its key role in gastric disease. J. Exp. Med. 179:1994;1653-1658.
27. Tombola F., Carlesso C., Szabò I., de Bernard M., Reyrat J.M., Telford J.L., Rappuoli R., Montecucco C., Papini E., Zoratti M. Helicobacter pylori vacuolating cytotoxin forms anion-selective channels in planar lipid bilayers: possible implications for the mechanism of cellular vacuolation. Biophys. J. 76:1999;1401-1409.
28. Wall J., Golding C.A., van Veen M., O'Shea P. The use of fluoresceinphosphatidylethanolamine (FPE) as a real-time probe for peptide-membrane interactions. Mol. Membr. Biol. 12:1995a;183-192.
29. Wall J., Ayoub F., O'Shea P. Interactions of macromolecules with the mammalian cell surface. J. Cell Sci. 108:1995b;2673-2682.