Sulfonation of polyvinylidene difluoride resin and its application in extraction of restriction enzymes from DNA digestion solutions

Analytical Biochemistry

Volumn 322, Issue 1, 2003, Pages 99-103

  Yang, Jing ^a   Dong, Chunxia ^a   Huang, Xu ^a   Zhao, Jindong ^a  

^a PEKING UNIVERSITY   (China)

Author keywords

DNA digestion; Enzyme extraction; PVDF; Restriction enzymes; Sulfonated PVDF

Indexed keywords

CLONE CELLS; CLONING; DNA; ENZYME ACTIVITY; EXTRACTION; SULFONATION;

DNA DIGESTION; ENZYME PURIFICATION; EXTRACTION PROCEDURE; MODERATELY HIGH TEMPERATURE; POLYVINYLIDENE DIFLUORIDE; PVDF; RESTRICTION ENZYMES; SULFONATED PVDF;

RESINS;

ENZYME; FLUORIDE; POLYVINYLIDINE DIFLUORIDE; RESIN; RESTRICTION ENDONUCLEASE; SULFURIC ACID; UNCLASSIFIED DRUG;

ADSORPTION KINETICS; ARTICLE; CHEMICAL MODIFICATION; CHEMICAL REACTION; DNA DEGRADATION; DNA EXTRACTION; ENZYME PURIFICATION; HIGH TEMPERATURE; INCUBATION TIME; MOLECULAR CLONING; PRIORITY JOURNAL; SULFONATION;

EID: 0141869694     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(03)00463-9     Document Type: Article

References (12)

1. Sambrook J., Fritch E.F., Maniatis T. Molecular Cloning, A Laboratory Manual. 1989;Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
2. Mozdzanowski J., Hembach P., Speicher D.W. High yield electroblotting onto polyvinylidene difluoride membranes from polyacrylamide gels. Electrophoresis. 13:1992;59-64.
3. Reim D.F., Speicher D.W. Microsequence analysis of electroblotted proteins. II. Comparison of sequence performance on different types of PVDF membranes. Anal. Biochem. 207:1992;19-23.
4. Barton A.F.M. CRC handbook of polymer-liquid interaction parameters and solubility parameter. 1990;CRC Press, Boca Raton, FL.
5. Rodrigues J.D., Combrink J., Brandt W.F. Derivatization of polyvinylidene difluoride membranes for the solid-phase sequence analysis of a phosphorylated sea urchin embryo histone H1 peptide. Anal. Biochem. 216:1994;365-372.
6. Aoshima R., Kanda Y., Takada A., Yamashita A. Sulfonated poly(vinylidene fluoride) as a biomaterial: immobilization of urokinase and biocompatibility. J. Biomed. Mater. Res. 16:1982;289-299.
7. Arcangioli B., Pochet S., Sousa R., Huynh-Dinh T. Preparation and use of a universal primed Sepharose for the purification of DNA-binding proteins. Eur. J. Biochem. 179:1989;359-363.
8. Burgess R.R. Use of polyethyleneimine in purification of DNA-binding proteins. Methods Enzymol. 208:1991;3-10.
9. Richards G.N. Ion-exchange membrane by sulfonation of poly(vinylidene fluoride) films. J. Appl. Polymer Sci. 8:1964;2269-2280.
10. Studies F.W., Rosenberg A.H., Dunn J.J., Dendorff J.W. Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol. 185:1991;60-89.
11. Buzby J.S., Porter R.D., Stevens S.E. Expression of the Escherichia coli lacZ gene on a plasmid vector in a cyanobacterium. Science. 230:1985;805-807.
12. Porte-Durrieu M.C., Aymes-Chodur C., Betz N., Baquey C. Development of "heparin-like" polymers using swift heavy ion and gamma radiation. I. Preparation and characterization of the materials. J. Biomed. Mater. Res. 52:2000;119-127.