Evaluation of a novel method based on PCR Restriction Fragment Length Polymorphism Analysis of the tuf gene for the identification of Staphylococcus species

Journal of Microbiological Methods

Volumn 55, Issue 2, 2003, Pages 465-469

  Kontos, F ^a   Petinaki, E ^a   Spiliopoulou, I ^b   Maniati, M ^a   Maniatis, A N ^a  

^a UNIVERSITY OF THESSALY   (Greece)

^b UNIVERSITY OF PATRAS   (Greece)

Author keywords

Staphylococcus species; tuf gene

Indexed keywords

ANALYTIC METHOD; ARTICLE; BACTERIAL GENE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; BIOASSAY; EVALUATION; GENE AMPLIFICATION; GENE SEQUENCE; MOLECULAR GENETICS; NONHUMAN; NUCLEOTIDE SEQUENCE; PATHOGENESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SENSITIVITY ANALYSIS; SEQUENCE ANALYSIS; STAPHYLOCOCCUS; TUF GENE; VALIDATION PROCESS;

STAPHYLOCOCCUS;

EID: 0141866915     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7012(03)00173-8     Document Type: Article

References (13)

1. Bannerman T.L., Kleeman K.T., Kloos W.E. Evaluation of the Vitek system's Gram-positive identification card for species identification of coagulase-negative staphylococci. J. Clin. Microbiol. 31:1993;1322-1325.
2. Dryden M.S., Talsania H.G., Martin S., Cunningham M., Richardson J.F., Cookson B., Marples R.R., Phillips I. Evaluation of methods for typing coagulase-negative staphylococci. J. Med. Microbiol. 37:1992;109-117.
3. Grant C.E., Sewell D.L., Pfaller M., Bumgardner R.V., Williams J.A. Evaluation of two commercial systems for identification of coagulase-negative staphylococci to species level. Diagn. Microbiol. Infect. Dis. 18:1994;1-5.
4. Gribaldo S., Cookson B., Saunders N., Marples R., Stanley J. Rapid identification by specific PCR of coagulase-negative staphylococcal species important in hospital infection. J. Med. Microbiol. 46:1997;45-53.
5. Grosserode M.H., Wenzel R.P. The continuing importance of staphylococci as major hospital pathogens. J. Hosp. Infect. 19:1991;3-17.
6. Ieven M., Verhoeven J., Pattyn S.R., Goossens H. Rapid and economical method for species identification of clinically significant coagulase-negative staphylococci. J. Clin. Microbiol. 33:1995;1060-1063.
7. Jarvis W.R., Martone W.J. Predominant pathogens in hospital infections. J. Antimicrob. Chemother. 29:1992;19-24.
8. Kloos W.E., Bannermn T.L. Staphylococcus and Micrococcus. Murray P.R., Barron E.J., Pfaller M.A., Tenover F.C., Yolken R.H. Manual of Clinical Microbiology. 1995;282-298 American Society for Microbiology, Washington, DC.
9. Kloos W.E., Schleifer K.H. Simplified scheme for routine identification of human Staphylococcus species. J. Clin. Microbiol. 1:1975;82-88.
10. Martineau F., Picard F.J., Ke D., Paradis S., Roy P.H., Quellette M., Bergeron M.G. Development of a PCR assay for identification of staphylococci at genus and species levels. J. Clin. Microbiol. 39:2001;2541-2547.
11. Monsen T., Ronnmark M., Olofsson C., Wistrom J. An inexpensive and reliable method for routine identification of staphylococcal species. Eur. J. Clin. Microbiol. Infect. Dis. 17:1998;327-335.
12. Poyart C., Quesne G., Boumaila C., Trieu-Cuot P. Rapid and accurate species-level identification of coagulase-negative staphylococci by using the sodA gene as a target. J. Clin. Microbiol. 39:2001;4296-4301.
13. Yugueros J., Temprano A., Sanchez M., Luengo J.M., Naharro G. Identification of Staphylococcus spp. By PCR-restriction fragment length polymorphism of gap gene. J. Clin. Microbiol. 39:2001;3693-3695.