A novel stationary phase: Capillary-channeled polymer (C-CP) fibers for HPLC separations of proteins

Journal of Chromatographic Science

Volumn 41, Issue 9, 2003, Pages 475-479

  Nelson, Dwella K ^a   Marcus, R Kenneth ^a  

^a CLEMSON UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACETONITRILE; HEMOCYANIN; HEMOGLOBIN; MYOGLOBIN; POLYMER; PROPANOL; PROTEIN; SUPEROXIDE DISMUTASE; TRIFLUOROACETIC ACID; WATER;

ANALYTIC METHOD; ARTICLE; CHEMICAL ANALYSIS; CHROMATOGRAPHY; FIBER; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; POLYMERIZATION; PROTEIN ANALYSIS;

EID: 0141782429     PISSN: 00219665     EISSN: None     Source Type: Journal    
DOI: 10.1093/chromsci/41.9.475     Document Type: Article

References (18)

1. M. Green and K.H. Brackmann. The application of high-performance liquid chromatography for the resolution of proteins encoded by the human adenovirus type 2 cell transformation region. In HPLC of Proteins and Peptides: International Symposium on HPLC of Proteins and Peptides. M.T.W. Hearn, F. Regnier, and C.T. Wehr, Eds. Academic Press, New York, NY, 1983, pp. 73-80.
2. J.G. Voet and C. Pratt. Fundamentals of Biochemistry. John Wiley & Sons, New York, NY, 2002, pp. 161-91.
3. B.S. Welinder, T. Kornfelt, and H.H. Sorensen. Stationary phases: the achilles heel in HPLC of protein pharmaceuticals. Anal. Chem. 67: 40-43 (1995).
4. R.L. Cunico, K.M. Gooding, and T. Wehr. Basic HPLC and CE of Biomolecules. Bay Bioanalytical Laboratory, Hercules, CA, 1998, pp. 67,156, 171-74,192.
5. H.G. Lee, J.L. Tseng, R.R. Becklin, and D.M Desiderio. Preparative and analytical capillary zone electrophoresis analysis of native endorphins and enkephalins extracted from molecular mass of leucine enkephalin. Anal. Biochem. 229: 188-79 (1995).
6. Y. Hue and Peter W. Carr. Synthesis and characterization of new zirconia-based polymeric cation-exchange stationary phases for high performance liquid chromatography of proteins. Anal. Chem. 70:1734-43 (1998).
7. K.C. O'Connor, S. Ghatak, and B.D. Stollar. Use of hydrophobic interaction chromatography to separate recombinant antibody fragments from associated bacterial chaperone protein Gro EL. Anal. Biochem. 278: 239-41 (2000).
8. L.R. Snyder, J.J. Kirkland, and J.L. Glajch. Practical HPLC Method Development. John Wiley and Sons, New York, NY, 1997, pp. 341-46,509-16.
9. S.L. Wu, A. Figueroa, and B. Karger. Protein conformational effects in HIC: retention characterization and the role of mobile phase additives and stationary phase hydrophobicity. J. Chromatogr. A 371: 3-27 (1986).
10. R.K. Marcus, W.C. Davis, B. Knippel, L. LaMotte, T. Hill, D. Perahia, and J.D. Jenkins. Capillary-channeled polymer fibers as stationary phases in liquid chromatography. J. Chromatogr. A 986: 17-31 (2002).
11. M. Czok and G. Guiochon. Aligned fiber columns for size exclusion chromatography. Anal. Chem. 53: 1318 (1981).
12. Y. Yang, Ajoy Velayudhan, C. Ladisch, and M.R. Ladisch. Protein chromatography using a continuous stationary phase. J. Chromatogr. A 598: 169-80 (1992).
13. J. Jinno, J. Wu, H. Sawada, and Y. Kiso. Cellulose acetate fibers as stationary phase in capillary electrochromatography. J. High Resolut. Chromatogr. 21: 617 (1998).
14. W.A. Haile and B.M. Phillips. Deep grooved polyester fiber for wet lay applications. Tappi J. 78: 139-42 (1995).
15. F.E. Regnier. High-performance liquid chromatography of biopolymers. Science 222: 245-52 (1983).
16. M.I. Aguilar and M.T.W. Hearn. High-resolution reversed phase high-performance liquid chromatography of peptides and proteins. Methods Enzymol. 270:3-26 (1996).
17. J.J. Kirkland, F.A. Truszkowski, and R.D. Ricker. Atypical silica-based packings for high-performance liquid chromatography. J. Chromatogr. A 965: 25-34 (2002).
18. V.E. Turula and J.A. de Haseth. Particle beam LC/FT-IR spectrometry studies of biopolymer conformations in reversed-phase HPLC separations: native globular proteins. Anal. Chem. 68: 629-38 (1996).