Membrane potential manipulation in synaptic plasma membrane vesicles for studying neurotransmitter uptake and release

Brain Research Protocols

Volumn 1, Issue 1, 1997, Pages 1-12

  Goncalves P P ^a   Carvalho, A P ^b  

^a UNIVERSITY OF AVEIRO   (Portugal)

^b UNIVERSITY OF COIMBRA   (Portugal)

Author keywords

Membrane potential; Neurotransmitter uptake and release; Synaptic plasma membranes

Indexed keywords

4 AMINOBUTYRIC ACID; ACETYLCHOLINESTERASE; ADENOSINE TRIPHOSPHATASE (CALCIUM MAGNESIUM); ADENOSINE TRIPHOSPHATASE (POTASSIUM SODIUM); AMINOOXYACETIC ACID; CATION; GLUCOSE 6 PHOSPHATASE; NEUROTRANSMITTER; POTASSIUM ION; SODIUM ION; TETRAPHENYLPHOSPHONIUM; TETRODOTOXIN; TRITIUM; VALINOMYCIN; VERATRIDINE;

ANIMAL TISSUE; ARTICLE; BRAIN CORTEX; CONTROLLED STUDY; ENZYME ACTIVITY; MALE; MORPHOLOGY; NEUROTRANSMITTER RELEASE; NEUROTRANSMITTER UPTAKE; NONHUMAN; PRIORITY JOURNAL; SHEEP; SYNAPSE VESICLE; SYNAPTIC POTENTIAL;

EID: 0141770448     PISSN: 1385299X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1385-299X(94)01118-Y     Document Type: Article

References (20)

1. Chan, P.H., Kerlan, R. and Fishman, R.A., Intracellular volume of osmotically regulated C-6 glioma cells, J. Neurosci. Res., 8 (1982) 67-72.
2. Cotman, C.W. and Matthews, D.A., Synaptic plasma membranes from rat brain synaptosomes: Isolation and partial characterization, Biochim. Biophys. Acta, 249 (1971) 380-394.
3. Flewelling, R.F. and Hubbell, W.L., Hydrophobic ion interactions with membranes. Thermodynamic analysis of tetraphenylphosphonium binding to vesicles, Biophys. J., 49 (1986) 531-540.
4. Flewelling, R.F. and Hubbell, W.L., The membrane dipole potential in a total membrane potential model. Applications to hydrophobic ion interactions with membranes, Biophys. J., 49 (1986) 541-552.
5. Gonçalves, P.P. and Carvalho, A.P., Effects of anions on the uptake and release of the γ-aminobutyric acid by isolated synaptic plasma membranes, Neurochem. Int., 25 (1994) 483-492.
6. 3H]GABA release from isolated synaptic plasma membrane vesicles, Neurochem. Res., 20 (1995) 177-186.
7. 3H]γ-aminobutyric acid by synaptic plasma membrane vesicles, Mol. Brain Res., 32 (1995) 161-165.
8. 3H]GABA by synaptic plasma membrane vesicles, Neurochem. Int., 17 (1990) 401-413.
9. Gornall, A.G., Bardawill, Ch.J. and David, M.M., Determination of serum proteins by means of the biuret reaction, J. Biol. Chem., 177 (1949) 751-766.
10. Gurd, J.W., Jones, L.R., Mahler, H.R. and Moore, W.J., Isolation and partial characterization of rat brain synaptic plasma membranes, J. Neurochem., 22 (1974) 281-290.
11. Jones, D.H. and Matus, A.I., Isolation of synaptic plasma membrane from brain by combined flotation sedimentation density gradient centrifugation, Biochim. Biophys. Acta, 356 (1974) 276-287.
12. Kamo, N., Muratsugu, M., Hongoh, R. and Kobatake, Y., Membrane potential of mitochondria measured with an electrode sensitive to tetraphenyl phosphonium and relationship between proton electrochemical potential and phosphorylation potential in steady state, J. Membrane Biol., 49 (1979) 105-121.
13. Kant, J.A. and Steck, Th.L., Cation-impermeable inside-out and right-side-out vesicles from human erythrocyte membranes, Nature New Biol., 240 (1972) 26-28.
14. Kauppinen, R.A., Sihra, T.S. and Nicholls, D.G., Divalent cation modulation of the ionic permeability of the synaptosomal plasma membrane, Biochim. Biophys. Acta, 860 (1986) 178-184.
15. Morgan, I.G., Wolfe, L.S., Mandel, P. and Gombos, G., Isolation of plasma membranes from rat brain, Biochim. Biophys. Acta, 241 (1971) 737-751.
16. Santos, M.S., Gonçalves, P.P. and Carvalho, A.P., Compartmentation and release of exogenous GABA in sheep brain synaptosomes. Neurochem. Res., 12 (1987) 297-304.
17. 2+ buffers to control the synaptosomal membrane potential, Biochem. J., 225 (1985) 671-680.
18. +/sugar symport in yeast under conditions of depolarized plasma membrane, J. Bioenerg. Biomemembr., 21 (1989) 321-334.
19. Sun, G.Y., Huang, H.-M., Kellecher, J.A., Stubbs, E.B. Jr. and Sun, A.Y., Marker enzymes, phospholipids and acyl group composition of a somal plasma membrane fraction isolated from rat cerebral cortex: A comparison with microsomes and synaptic plasma membranes, Neurochem. Int., 12 (1988) 69-77.
20. Tsutsumi, T., Kobayashi, T., Ueda, H., Yamauchi, E., Watanabe, S. and Okuyama, H., Lysophosphoinositide-specific phospholipase C in rat brain synaptic plasma membranes, Neurochem. Res., 19 (1994) 399-406.