Isolation and characterization of Bacillus sp. BP-6 LipA, a ubiquitous lipase among mesophilic Bacillus species

Letters in Applied Microbiology

Volumn 37, Issue 4, 2003, Pages 354-359

  Ruiz, C ^a   Pastor, F I Javier ^a   Diaz, Pilar ^a  

^a UNIVERSITY OF BARCELONA   (Spain)

Author keywords

Bacillus; Carboxylesterase; Lipase

Indexed keywords

BACTERIOLOGY; ESCHERICHIA COLI; GENES; LIPASES;

AMINO ACID SEQUENCE; B.SUBTILIS; BACILLUS SP; BACILLUS SPECIES; CARBOXYLESTERASES; CODING GENES; ISOLATION AND CHARACTERIZATION; MEGATERIUM; MESOPHILIC; TRIBUTYRIN;

CLONING;

TRIACYLGLYCEROL LIPASE;

BACTERIUM;

ARTICLE; BACILLUS MEGATERIUM; BACILLUS SUBTILIS; BACTERIUM ISOLATION; BIOCHEMISTRY; BIOTECHNOLOGY; CONTROLLED STUDY; DIRECT POLYMERASE CHAIN REACTION; ENZYME ACTIVITY; ENZYME ISOLATION; ESCHERICHIA COLI; GENETIC CODE; INVERSE POLYMERASE CHAIN REACTION; MESOPHYLL; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; SEQUENCE HOMOLOGY;

BACILLUS; BACILLUS MEGATERIUM; BACILLUS SP.; BACILLUS SP. BP-6; BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; POSIBACTERIA;

EID: 0141762453     PISSN: 02668254     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1472-765X.2003.01413.x     Document Type: Article

References (22)

1. Altschul, S.F., Madden, T.L., Schaffer, A.A., Zhang, J., Zhang, Z., Miller, W. and Lipman, D.J. (1997) Gapped-BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research 25, 3389-3402.
2. Arpigny, J.L. and Jaeger, K.E. (1999) Bacterial lipolytic enzymes: classification and properties. Biochemical Journal 343, 177-183.
3. Dartois, V., Baulard, A., Schanck, K. and Colson, C. (1992) Cloning, nucleotide sequence and expression in Escherichia coli of a lipase gene from Bacillus subtilis 168. Biochimica et Biophysica Acta 1131, 253-260.
4. Diaz, P., Prim, N. and Pastor, F.I.J. (1999) Direct fluorescence-based lipase activity assay. BioTechniques 27, 696-700.
5. Eggert, T., Pencreac'h, G., Douchet, I., Verger, R. and Jaeger, K.E. (2000) A novel extracellular esterase from Bacillus subtilis and its conversion to a monoacylglycerol hydrolase. European Journal of Biochemistry 267, 6459-6469.
6. Jaeger, K.E., Dijkstra, B.W. and Reetz, M.T. (1999) Bacterial biocatalysts: molecular biology, three-dimensional structures, and biotechnological applications of lipases. Annual Reviews of Microbiology 53, 315-351.
7. Kim, H.K., Park, S.Y., Lee, J.K. and Oh, T.K. (1998) Gene cloning and characterisation of thermostable lipase from Bacillus stearothermophilus L1. Bioscience, Biotechnology and Biochemistry 62, 66-71.
8. Kouker, G. and Jaeger, K.E. (1987) Specific and sensitive plate assay for bacterial lipases. Applied and Environmental Microbiology 53, 211-213.
9. Lee, D.W., Koh, Y.S., Kim, K.J., Kim, B.C., Choi, H.J., Kim, D.S., Suhartono, M.T. and Pyun, Y.R. (1999) Isolation and characterisation of a thermophilic lipase from Bacillus thermoleovorans ID-1. FEMS Microbiology Letters 179, 393-400.
10. Moeller, B., Vetter, R., Wilke, D. and Foullois, B. (1992) Alkane lipases from Bacillus and the cloning of their genes. Patent number WO9116422. Applicant(s) Kali Chemie A.-G.
11. Nielsen, H., Engelbrecht, J., Brunak, S. and von Heijne, G. (1997) Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Engineering 10, 1-6.
12. Nthangeni, M.B., Patterton, H.G., Van Tonder, A., Vergeer, W.P. and Litthauer, D. (2001) Over-expression and properties of a purified recombinant Bacillus licheniformis lipase: a comparative report on Bacillus lipases. Enzyme and Microbial Technology 28, 705-712.
13. Pohlenz, H.D., Boidol, W., Schüttke, I. and Streber, W. (1992) Purification and properties of an Arthrobacter oxydans P52 carbamate hydrolase specific for the herbicide phenmedipham and nucleotide sequence of the corresponding gene. Journal of Bacteriology 174, 6600-6607.
14. Prim, N., Blanco, A., Martínez, J., Pastor, F.I.J. and Diaz, P. (2000) estA, a gene coding for a cell-bound esterase from Paenibacillus sp. BP-23, is a new member of the bacterial subclass of type B carboxylesterases. Research in Microbiology 151, 303-312.
15. Prim, N., Pastor, F.I.J. and Diaz, P. (2001) Cloning and characterisation of a bacterial cell-bound type B carboxylesterase from Bacillus sp. BP-7. Current Microbiology 42, 237-240.
16. Ruiz, C., Pastor, F.I.J. and Diaz, P. (2002) Analysis of Bacillus megaterium lipolytic system and cloning of LipA, a novel subfamily I.4 bacterial lipase. FEMS Microbiology Letters 217, 263-267.
17. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
18. Schmidt-Dannert, C. (1999) Recombinant microbial lipases for biotechnological applications. Bioorganic and Medical Chemistry 7, 2123-2130.
19. Schmidt-Dannert, C., Rúa, M.L., Atomi, H. and Schmid, R.D. (1996) Thermoalkalophilic lipase of Bacillus thermocatenulatus. I. Molecular cloning, nucleotide sequence, purification and some properties. Biochimica et Biophysica Acta 1301, 105-114.
20. Wang, C.S. and Hartsuck, J.A. (1993) Bile salt-activated lipase. A multiple function lipolytic enzyme. Biochimica et Biophysica Acta 1166, 1-19.
21. Yamamoto, H., Uchiyama, S. and Sekiguchi, J. (1996) The Bacillus subtilis chromosome region near 78° contains the genes encoding a new two-component system, three ABC transporters and a lipase. Gene 181, 147-151.
22. Zock, J., Cantwell, C., Swartling, J., Hodges, R., Pohl, T., Sutton, K., Rosteck, P., McGilvray, D. and Queener, S. (1994) The Bacillus subtilis pnbA gene encoding p-nitrobenzyl esterase: cloning, sequence and high-level expression in Escherichia coli. Gene 151, 37-43.