Novel method for the covalent immobilization of oligonucleotides via Diels-Alder bioconjugation

Nucleosides, Nucleotides and Nucleic Acids

Volumn 22, Issue 5-8, 2003, Pages 1495-1497

  Latham Timmons, Hallie A ^a   Wolter, Andreas ^a   Roach, J Shawn ^a   Giare, Rubina ^a   Leuck, Michael ^a  

^a Proligo LLC   (United States)

Author keywords

Aqueous Diels Alder reaction; Bioconjugation; Surface immobilization

Indexed keywords

CYCLOALKENE; GLASS; MALEIMIDE DERIVATIVE; OLIGONUCLEOTIDE;

CONFERENCE PAPER; CONJUGATION; COVALENT BOND; DIELS ALDER REACTION; MATERIAL COATING; SURFACE PROPERTY; SYNTHESIS; TECHNIQUE;

CHEMISTRY, ORGANIC; GLASS; OLIGONUCLEOTIDE PROBES; OLIGONUCLEOTIDES; SURFACE PROPERTIES;

BETULACEAE;

EID: 0141707921     PISSN: 15257770     EISSN: None     Source Type: Journal    
DOI: 10.1081/NCN-120023019     Document Type: Conference Paper

References (2)

1. Hill, K.W.; Taunton-Rigby, J.; Carter, J.D.; Kropp, E.; Vagle, K.; Pieken, W.; McGee, D.P.C.; Husar, G.M.; Leuck, M.; Anziano, D.J.; Sebesta, D.P. Diels-Alder bioconjugation of diene-modified oligonucleotides. J. Org. Chem. 2001, 66, 5352-5358.
2. Pieken, W.; Wolter, A.; Sebesta, D.P.; Leuck, M.; Latham-Timmons, H.A.; Pilon, J.; Husar, G.M. Method for immobilizing oligonucleotides employing the cycloaddition bioconjugation method. PCT Int. Appl. WO 01/84234 A1, November 8, 2001.