A quantitative ELISA-based co-culture angiogenesis and cell proliferation assay

APMIS

Volumn 111, Issue 6, 2003, Pages 658-668

  Friis, Tina ^a,b   Sørensen, Birgitte Kjær ^a   Engel, Anne Marie ^b   Rygaard, Jørgen ^b   Houen, Gunnar ^a  

^a STATENS SERUM INSTITUT   (Denmark)

^b Rigshospitalet   (Denmark)

Author keywords

Angiogenesis; CD 31; Co culture; Collagen IV; ELISA; Endothelial tube formation; Von Willebrand factor

Indexed keywords

ACETYLSALICYLIC ACID; ANGIOGENESIS INHIBITOR; CD31 ANTIGEN; COLLAGEN TYPE 4; FUMAGILLOL CHLOROACETYLCARBAMATE; IBUPROFEN; INDOMETACIN; NONSTEROID ANTIINFLAMMATORY AGENT; SURAMIN; VASCULOTROPIN; VON WILLEBRAND FACTOR;

ANGIOGENESIS; ARTICLE; BACTERIUM CULTURE; CELL CULTURE; CELL PROLIFERATION; CELL STRUCTURE; CONTROLLED STUDY; DNA DETERMINATION; DRUG DETERMINATION; ENDOTHELIUM CELL; ENZYME LINKED IMMUNOSORBENT ASSAY; FIBROBLAST; HUMAN; HUMAN CELL; IMMUNOCHEMISTRY; PRIORITY JOURNAL; QUALITATIVE ANALYSIS; QUANTITATIVE DIAGNOSIS; UMBILICAL CORD;

ANGIOGENESIS INHIBITORS; CELL DIVISION; COCULTURE TECHNIQUES; DNA; DRUG EVALUATION, PRECLINICAL; ENDOTHELIUM, VASCULAR; ENZYME-LINKED IMMUNOSORBENT ASSAY; FIBROBLASTS; FLUORESCENT DYES; HUMANS; IMMUNOENZYME TECHNIQUES; INDOLES; MICROSCOPY, FLUORESCENCE; NEOVASCULARIZATION, PATHOLOGIC; NITROBLUE TETRAZOLIUM;

EID: 0141630341     PISSN: 09034641     EISSN: None     Source Type: Journal    
DOI: 10.1034/j.1600-0463.2003.1110609.x     Document Type: Article

References (18)

1. Folkman J, D'Amore PA. Blood vessel formation: what is its molecular basis? Cell 1996;87:1153-5.
2. Klagsbrun M, Moses MA. Molecular angiogenesis. Chem Biol 1999;6:R217-24.
3. 3H) thymidine techniques to measure cell proliferation. Cell Tissue Kinet 1981;14:111-20.
4. Herzenberg LA, Sweet RG, Herzenberg LA. Fluorescence-activated cell sorting. Sci Am 1976;234:108-17.
5. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:55-63.
6. Faraji-Shadan F, Bowman PD. An enzyme immunoassay for estimating small numbers of cells. Anal Biochem 1989;177:259-62.
7. Nicosia RF, Ottinetti A. Modulation of microvascular growth and morphogenesis by reconstituted basement membrane gel in three-dimentional cultures of rat aorta: a comparative study of angiogenesis in matrigel, collagen, fibrin, and plasma clot. In Vitro Cell Dev Biol 1990;26:119-28.
8. Donovan D, Brown NJ, Bishop ET, Lewis CE. Comparison of three in vitro human "angiogenesis" assays with capillaries formed in vivo. Angiogenesis 2001;4:113-21.
9. Boyden S. The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leucocytes. J Exp Med 1962;115:453-66.
10. Nicosia R, Ottinetti A. Growth of microvessels in serum-free matrix culture of rat aorta. A quantitative assay of angiogenesis in vitro. Lab Invest 1990;63:155-22.
11. Bishop ET, Bell GT, Bloor S, Broom IJ, Hendry NFK, Wheatley DN. An in vitro model of angiogenesis: Basic features. Angiogenesis 1999;3:335-44.
12. Sauter B, Foedinger D, Sterniczky B, Wollf K, Rappersberger K. Immunoelectron microscopic characterization of human dermal lymphatic microvascular endothelial cells. Differential expression of CD 31, CD 34 and type IV collagen with lymphatic endothelial cells vs blood capillary endothelial cells in normal human skin, lymphangioma, and hemangioma in situ. J Histochem Cytochem 1998;46:165-76.
13. 1 phase of the cell cycle. Cancer Res 1994;54:2073-6.
14. Houen G. Mammalian Cu-containing amine oxidases (CAOs): new methods of analysis, structural relationships, and possible functions. APMIS 1999;96(Suppl):S1-46.
15. Jones MK, Wang H, Peskar BM, Levin E, Itani RM, Sarfeh IJ, et al. Inhibition of angiogenesis by nonsteroidal anti-inflammatory drugs: insight into mechanisms and implications for cancer growth and ulcer healing. Nat Med 1999;5:1418-23.
16. O'Reilly MS, Holmgren L, Shing Y, Chen C, Rosenthal RA, Moses M, et al. Angiostatin: a novel angiogenesis inhibitor that mediates the suppression of metastases by a Lewis lung carcinoma. Cell 1994;79:315-28.
17. Cao Y, Ji RW, Davidson D, Schaller J, Marti D, Sõhndel S, et al. Kringle domains of human angiostatin. Characterization of the anti-proliferative activity on endothelial cells. J Biol Chem 1996;271:29461-7.
18. Pike SE, Yao L, Setsuda J, Jones KD, Cherney B, Appella E, et al. Calreticulin and calreticulin fragments are endothelial cell inhibitors that suppress tumor growth. Blood 1999;94:2461-8.