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note
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The PL microscope setup was constructed such that the emission from the microspheres, collected through a microscope objective (NA = 0.45 or 1.25), could be simultaneously imaged with a charge-coupled device (CCD) as well as detected and analyzed using a spectrophotometer. Using the same setup, illumination with a white light source allowed optical (transmission mode) imaging of the sample.
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For the PL measurements the exciting laser beam was focused on the sample and the emission was collected backwards using a microscope objective (NA = 0.45). A 400 nm highpass filter was used in order to block out the scattered light from the pump laser. The emission from individual CdSe QDMSs was selected by a pinhole (20-500 μm in diameter) and guided with a quartz fiber optic to the entrance slit of a spectrophotometer equipped with a monochromator (150 grooves/mm) and a liquid nitrogen cooled back-illuminated CCD detector.
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