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12
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0141858931
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note
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2.
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13
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0141524275
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note
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2 was obtained by air oxidation at pH 8.8. Formation of the dimer was confirmed by gel filtration (see Supporting Information) and MALDI-TOF MS.
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14
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0141524276
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+ fragment is cleaved (presumably during the laser ionization process). Together with spectroscopic evidence, this shows that Ru is bound, as designed, to the His21 pairs and not His42.
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15
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0141858932
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note
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2 in a 1 × 1 cm cuvette was degassed by a series of freeze-pump-thaw cycles.
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16
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0032539247
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Luo, J.; Wishart, J. F.; Isied, S. S. J. Am. Chem. Soc. 1998, 120, 12970.
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Luo, J.1
Wishart, J.F.2
Isied, S.S.3
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17
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0141524271
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note
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The 532 nm, 10 ns, 100 mJ output of a pulsed Nd:YAG laser was used for excitation. The probe light (Xe-flashlamp) was passed through a monochromator and detected with a PMT. The kinetics was monitored at 428 nm and averaged over 256 shots. The protein (50 μM aRC) was thoroughly degassed by a series of freeze-pump-thaw cycles.
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18
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0027416831
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Fom the measured redox potentials and the excited energy of bound-Ru (2. 1 eV), the driving forces for the forward and back ET, are -0.8 and -1.3 eV, respectively. It is therefore predicted that the forward reaction is nearly activationless, while the back reaction is in the Marcus inverted region. Farid, R. S.; Moser, C. C.; Dutton, P. L. Curr. Opin. Struct. Biol. 1993, 3, 225-233.
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(1993)
Curr. Opin. Struct. Biol.
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Farid, R.S.1
Moser, C.C.2
Dutton, P.L.3
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19
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0141524274
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note
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III by reduced cyt c is -0.055 eV, and that of oxidation of heme by naphthoquinone-2-sulfonate is -0.95 eV. Given the CSS lifetime of 70 ns, even at 20 μM of cyt c and the quinone, the interception efficiency of the CSS, assuming a diffusion-controlled rate, would be in the range of ∼1%.
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21
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0033984117
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(b) Jones, G., II; Vullev, V.; Braswell, E. H.; Zhu, D. J. Am. Chem. Soc. 2000, 122, 388.
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Jones G. II1
Vullev, V.2
Braswell, E.H.3
Zhu, D.4
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