메뉴 건너뛰기




Volumn 125, Issue 39, 2003, Pages 11814-11815

Mimicking photosynthesis in a computationally designed synthetic metalloprotein

Author keywords

[No Author keywords available]

Indexed keywords

HELIX LOOP HELIX PROTEIN; METALLOPROTEIN; RUTHENIUM;

EID: 0141620306     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja0292142     Document Type: Article
Times cited : (19)

References (22)
  • 9
    • 0141747397 scopus 로고    scopus 로고
    • Ph.D. Thesis, Rutgers, The State University of New Jersey, Newark, NJ
    • Cristian, L. Ph.D. Thesis, Rutgers, The State University of New Jersey, Newark, NJ, 2002.
    • (2002)
    • Cristian, L.1
  • 12
    • 0141858931 scopus 로고    scopus 로고
    • note
    • 2.
  • 13
    • 0141524275 scopus 로고    scopus 로고
    • note
    • 2 was obtained by air oxidation at pH 8.8. Formation of the dimer was confirmed by gel filtration (see Supporting Information) and MALDI-TOF MS.
  • 14
    • 0141524276 scopus 로고    scopus 로고
    • note
    • + fragment is cleaved (presumably during the laser ionization process). Together with spectroscopic evidence, this shows that Ru is bound, as designed, to the His21 pairs and not His42.
  • 15
    • 0141858932 scopus 로고    scopus 로고
    • note
    • 2 in a 1 × 1 cm cuvette was degassed by a series of freeze-pump-thaw cycles.
  • 17
    • 0141524271 scopus 로고    scopus 로고
    • note
    • The 532 nm, 10 ns, 100 mJ output of a pulsed Nd:YAG laser was used for excitation. The probe light (Xe-flashlamp) was passed through a monochromator and detected with a PMT. The kinetics was monitored at 428 nm and averaged over 256 shots. The protein (50 μM aRC) was thoroughly degassed by a series of freeze-pump-thaw cycles.
  • 18
    • 0027416831 scopus 로고
    • Fom the measured redox potentials and the excited energy of bound-Ru (2. 1 eV), the driving forces for the forward and back ET, are -0.8 and -1.3 eV, respectively. It is therefore predicted that the forward reaction is nearly activationless, while the back reaction is in the Marcus inverted region. Farid, R. S.; Moser, C. C.; Dutton, P. L. Curr. Opin. Struct. Biol. 1993, 3, 225-233.
    • (1993) Curr. Opin. Struct. Biol. , vol.3 , pp. 225-233
    • Farid, R.S.1    Moser, C.C.2    Dutton, P.L.3
  • 19
    • 0141524274 scopus 로고    scopus 로고
    • note
    • III by reduced cyt c is -0.055 eV, and that of oxidation of heme by naphthoquinone-2-sulfonate is -0.95 eV. Given the CSS lifetime of 70 ns, even at 20 μM of cyt c and the quinone, the interception efficiency of the CSS, assuming a diffusion-controlled rate, would be in the range of ∼1%.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.