Fluorescence in situ hybridization in diluted and flow cytometrically sorted boar spermatozoa using specific DNA direct probes labelled by nick translation

Reproduction

Volumn 126, Issue 3, 2003, Pages 317-325

  Parrilla, I ^a   Vazquez J M ^a   Oliver Bonet, M ^b   Navarro, J ^b   Yelamos, J ^c   Roca, J ^a   Martinez E A ^a  

^a UNIVERSITY OF MURCIA   (Spain)

^b UNIVERSITY OF BARCELONA   (Spain)

^c HOSPITAL UNIVERSITARIO VIRGEN DE LA ARRIXACA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

DNA;

ANIMAL CELL; ARTICLE; CELL SELECTION; CELL SEPARATION; CHROMOSOME 1; CONTROLLED STUDY; DILUTION; DNA NICK TRANSLATION; DNA PROBE; FLOW CYTOMETRY; FLUORESCENCE IN SITU HYBRIDIZATION; GENETIC PURITY; NONHUMAN; NUCLEIC ACID ANALYSIS; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SPERMATOZOON; SWINE; VALIDATION PROCESS; X CHROMOSOME; Y CHROMOSOME;

ANIMALIA; SUS SCROFA;

EID: 0141613853     PISSN: 14701626     EISSN: None     Source Type: Journal    
DOI: 10.1530/rep.0.1260317     Document Type: Article

References (30)

1. Di Berardino D, Jovino V, Lioi MB, Scarfi MR and Burguete I (1996) Spontaneous rate of sister chromatid exchanges (SCEs) and BrdU dose-response relationships in mitotic chromosomes of goat (Capra hircus L.) Hereditas 124 137-143
2. Fugger EF, Black SH, Keyvanfar K and Schulman JD (1998) Births of normal daughters after MicroSort sperm separation and intrauterine insemination, in vitro fertilization, or intracytoplasmic sperm injection Human Reproduction 13 2367-2370
3. Hassanane M, Kovacs A, Laurent P, Lindblad K and Gustavsson I (1999) Simultaneous detection of X- and Y-bearing bull spermatozoa by double colour fluorescence in situ hybridization Molecular Reproduction and Development 53 407-412
4. Jantsch M (1989) Sus scrofa Mc1 satellite DNA. National Center for Biotechnology Information. X12696
5. Johnson LA (1991) Sex preselection in swine: altered sex ratios in offspring following surgical insemination of flow sorted X- or Y-bearing sperm Reproduction in Domestic Animals 26 309-314
6. Johnson LA (1997) Advances in gender preselection in swine Journal of Reproduction and Fertility Supplement 52 255-266
7. Johnson LA and Pinkel D (1986) Modification of a laser-based flow cytometer for high resolution DNA analysis of mammalian spermatozoa Cytometry 7 268-273
8. Johnson LA and Schulman JD (1994) The safety of sperm selection by flow cytometry Human Reproduction 9 758-759
9. Johnson LA and Welch GR (1999) Sex preselection: high-speed flow cytometry sorting of X and Y sperm for maximum efficiency Theriogenology 52 1323-1341
10. Johnson LA, Flook JP and Look MV (1987) Flow analysis of X- and Y-chromosome-bearing sperm for DNA using an improved preparation method and staining with Hoechst 33342 Gamete Research 17 203-212
11. Johnson LA, Aalbers JG and Grooten HJG (1988) Artificial insemination of swine: fecundity of boar semen stored in beltsville TS (BTS), modified modena (MM) or MR-A and inseminated on one, three and four days after collection Zuchthyg 23 49-55
12. Johnson LA, Flook JP and Hawk HW (1989) Sex preselection in rabbits: live births from X- and Y-sperm separated by DNA and cell sorting Biology of Reproduction 41 199-203
13. Johnson LA, Welch GR, Keyvanfar K, Dorfmann A, Fugger EF and Schulman JD (1993) Gender preselection in humans? Flow cytometric separation of X and Y spermatozoa for the prevention of X-linked diseases Human Reproduction 8 1733-1739
14. Kawarasaki T, Kohsaka T, Sone M, Yoshida M and Bamba K (1995) Detection of Y-bearing porcine spermatozoa by in situ hybridization using digoxigenin-labeled, porcine male-specific DNA probe produced by polymerase chain reaction Molecular Reproduction and Development 40 455-459
15. Kawarasaki T, Sone M, Yoshida M and Bamba K (1996) Rapid and simultaneous detection of chromosome Y- and 1-bearing porcine spermatozoa by fluorescence in situ hybridization Molecular Reproduction and Development 43 548-553
16. Kawarasaki T, Welch GR, Long CR, Yoshida M and Johnson LA (1998) Verification of flow cytometorically-sorted X-and Y-bearing porcine spermatozoa and reanalysis of spermatozoa for DNA content using the fluorescence in situ hybridization (FISH) technique Theriogenology 50 625-635
17. Langer PR, Waldrop AA and Ward DC (1981) Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic affinity probes Proceedings National Academy of Sciences USA 78 6633-6637
18. McGraw RA, Jacobson RJ and Akamatsu M (1988) A male-specific repeated DNA sequence in the domestic pig Nucleic Acids Research 16 10 389 (Abstract)
19. Perreault SD, Barbee RR, Elstein KH, Zucker RM and Keefer CL (1988) Interspecies differences in the stability of mammalian sperm nuclei assessed in vivo by sperm microinjection and in vitro by flow cytometry Biology of Reproduction 39 157-167
20. Piumi F, Vaiman D, Cribiu EP, Guerin B and Humblot P (2001) Specific cytogenetic labeling of bovine spermatozoa bearing X or Y chromosome using fluorescent in situ hybridization (FISH) Genetic Selection Evolution 33 89-98
21. Rens W, Yang F, Welch G, Revell S, O'Brien PCM, Solanky N, Johnson LA and Ferguson Smith MA (2001) An X-Y paint set and sperm FISH protocol that can be used for validation of cattle sperm separation procedures Reproduction 121 541-546
22. Rigby PW, Dieckmann M, Rhodes C and Berg P (1977) Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I Journal of Molecular Biology 113 237-251
23. Rodriguez H, Ohanian C and Bustos-Obregon E (1985) Nuclear chromatin decondensation of spermatozoa in vitro: a method for evaluating the fertilizing ability of ovine semen International Journal of Andrology 8 147-158
24. Rousseaux S and Chevret E (1995) In vitro decondensation of human spermatozoa for fluorescence in situ hybridisation Human Reproduction 10 2209-2213
25. Rubeš J, Vozdová M and Kubìcková S (1999) Aneuploidy in pig sperm: multicolor fluorescence in situ hybridization using probes for chromosomes 1, 10, and Y Cytogenetics and Cell Genetics 85 200-204
26. Seidel GE and Garner DL (2002) Current status of sexing mammalian spermatozoa Reproduction 124 733-743
27. Vazquez JM, Martinez EA, Parrilla I, Roca J, Gil MA and Vazquez JL (2003) Birth of piglets after deep intrauterine insemination with flow cytometrically sorted spermatozoa Theriogenology 59 1605-1614
28. Welch GR and Johnson LA (1999) Sex preselection: laboratory validation of the sperm sex ratio of flow sorted X- and Y-sperm by sort reanalysis for DNA Theriogenology 52 1343-1352
29. Welch GR, Waldbieser GC, Wall RJ and Johnson LA (1995) Flow cytometric sperm sorting and PCR to confirm separation of X- and Y-bearing bovine sperm Animal Biotechnology 6 131-139
30. Wyrobèk AJ, Robbing WA, Tang C, Kobayashi A, Baulch J, Weier H-U and Pinkel D (1993) Hierarchical organization of human sperm chromatin is critical factor in the detection of chromosomal aneuploidies by fluorescence in situ hybridization American Journal of Human Genetics Supplement 53 130a (Abstract)