Phenotypic changes resulting from distinct point mutations in the Azospirillum brasilense glnA gene, encoding glutamine synthetase

Applied and Environmental Microbiology

Volumn 69, Issue 9, 2003, Pages 5699-5701

  Van Dommelen, Anne ^a   Keijers, Veerle ^a   Wollebrants, An ^a   Vanderleyden, Jozef ^a  

^a UNIVERSITY OF LEUVEN   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

GENES; GENETIC ENGINEERING; MUTAGENESIS; NUCLEIC ACID SEQUENCES;

MUTATIONS;

MICROBIOLOGY;

AMMONIA; ARGININE; ASPARAGINE; ASPARTIC ACID; BETA GLUCURONIDASE; CYSTEINE; GLUTAMATE AMMONIA LIGASE; GLUTAMIC ACID; METHIONINE SULFOXIMINE;

ENZYME; MICROBIOLOGY; MUTATION;

AMINO ACID SEQUENCE; ARTICLE; AZOSPIRILLUM BRASILENSE; BACTERIUM MUTANT; BINDING SITE; CONTROLLED STUDY; GENE EXPRESSION; GENOTYPE; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; POINT MUTATION;

AMINO ACID SUBSTITUTION; ASPARAGINE; ASPARTIC ACID; AZOSPIRILLUM BRASILENSE; BINDING SITES; GENES, BACTERIAL; GLUTAMATE-AMMONIA LIGASE; POINT MUTATION; QUATERNARY AMMONIUM COMPOUNDS; RECOMBINANT FUSION PROTEINS;

AZOSPIRILLUM; AZOSPIRILLUM BRASILENSE; BACTERIA (MICROORGANISMS); FUNGI; MYXOGASTRIA; NEGIBACTERIA;

EID: 0141592641     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.69.9.5699-5701.2003     Document Type: Article

References (26)

1. Alibhai, M., and J. J. Villafranca. 1994. Kinetic and mutagenic studies of the role of the active site residues Asp-50 and Glu-327 of Escherichia coli glutamine synthetase. Biochemistry 33:682-686.
2. Bender, R. A., K. A. Janssen, A. D. Resnick, M. Blumenberg, F. Foor, and B. Magasanik. 1977. Biochemical parameters of glutamine synthetase from Klebsiella aerogenes. J. Bacteriol. 129:1001-1009.
3. Bozouklian, H., and C. Elmerich. 1986. Nucleotide sequence of the Azospirillum brasilense Sp7 glutamine synthetase structural gene. Biochimie 68:1181-1187.
4. Bozouklian, H., C. Fogher, and C. Elmerich. 1986. Cloning and characterization of the glnA gene of Azospirillum brasilense Sp7. Ann. Inst. Pasteur Microbiol. 137B:3-18.
5. Chaney, A. L., and E. P. Marbach. 1962. Modified reagents for determination of urea and ammonia. Clin. Chem. 8:130-132.
6. Coutts, G., G. Thomas, D. Blakey, and M. Merrick. 2002. Membrane sequestration of the signal transduction protein GlnK by the ammonium transporter AmtB. EMBO J. 21:536-545.
7. de Zamaroczy, M., A. Paquelin, and C. Elmerich, 1993. Functional organization of the glnB-glnA cluster of Azospirillum brasilense. J. Bacteriol. 175:2507-2515.
8. Eisenberg, D., H. S. Gill, G. M. Pfluegl, and S. H. Rotstein. 2000. Structure-function relationships of glutamine synthetases. Biochim. Biophys. 1477:122-145.
9. Gauthier, D., and C. Elmerich. 1977. Relationship between glutamine synthetase and nitrogenase in Spirillum lipoferum. FEMS Microbiol. Lett. 2:101-104.
10. Jefferson, R. A. 1987. Assaying chimeric genes in plants: the gus gene fusion system. Plant. Mol. Biol. Rep. 5:387-405.
11. Liaw, S. H., and D. Eisenberg. 1994. Structural model for the reaction mechanism of glutamine synthetase, based on five crystal structures of enzyme-substrate complexes. Biochemistry 33:675-681.
12. Liaw, S. H., C. Pan, and D. Eisenberg. 1993. Feedback inhibition of fully unadenylylated glutamine synthetase from Salmonella typhimurium by glycine, alanine, and serine. Proc. Natl. Acad. Sci. USA 90:4996-5000.
13. Liaw, S. H., I. Kuo, and D. Eisenberg. 1995. Discovery of the ammonium substrate site on glutamine synthetase, a third cation binding site. Protein Sci. 4:2358-2365.
14. Merrick, M. J., and R. A. Edwards. 1995. Nitrogen control in bacteria. Microbiol. Rev. 59:604-622.
15. Okon, Y., S. L. Albrecht, and R. H. Burris. 1976. Carbon and ammonia metabolism of Spirillum lipoferum. J. Bacteriol. 128:592-597.
16. Okon, Y., and J. Vanderleyden. 1997. Azospirillum-plant association. A case study for molecular mechanisms in phytostimulation, crop yield improvement, and rhizosphere ecology. ASM News 63:366-370.
17. + metabolism in Rhizobium-plant symbiosis. Microbiol. Mol. Biol. Rev. 66:203-222.
18. Tarrand, J. J., N. R. Krieg, and J. Döbereiner. 1978, A taxonomic study of the Spirillum lipoferum group, with the description of a new genus, Azospirillum gen. nov., and two species, Azospirillum lipoferum (Beijerinck) comb. nov. and Azospirillum brasilense sp. nov. Can. J. Microbiol 24:967-980.
19. Van Dommelen, A., E. Van Bastelaere, V. Keijers, and J. Vanderleyden. 1997, Genetics of Azospirillum brasilense with respect to ammonium transport, sugar uptake and chemotaxis. Plant Soil 194:155-160.
20. Van Dommelen, A. 1998. Ph.D. thesis. Katholieke Universiteit Leuven, Heverlee, Belgium.
21. Van Dommelen, A., V. Keijers, J. Vanderleyden, and M. de Zamaroczy. 1998. (Methyl)ammonium transport in the nitrogen-fixing bacterium Azospirillum brasilense. J. Bacteriol. 180:2652-2659.
22. Van Dommelen, A., R. De Mot, and J. Vanderleyden. 2001. Ammonium transport, unifying concepts and unique aspects. Aust. J. Plant Physiol. 28:1-9.
23. Van Dommelen, A., V. Keijers, E. Somers, and J. Vanderleyden. 2002. Cloning and characterisation of the Azospirillum brasilense ghtD gene and analysis of a glnD mutant. Mol. Genet. Genomics 266:813-820.
24. Vanstockem, M., K. Michiels, J. Vanderleyden, and A. Van Gool. 1987. Transposon mutagenesis of Azospirillum brasitense and Azospirillum lipoferum: physical analysis of Tn5 and Tn5-Mob insertion mutants. Appl. Environ. Microbiol. 53:410-415.
25. + by Azospirillum brasilense grown under nitrogen limitation and excess. J. Bacteriol. 169:4211-4214.
26. Zhang, Y., R. H. Burris, P. W. Ludden, and G. P. Roberts. 1994. Posttranslational regulation of nitrogenase activity in Azospirillum brasilense ntrBC mutants: ammonium and anaerobic switch-off occurs through independent signal transduction pathways. J. Bacteriol. 176:5780-5787.