Cloning and expression of mitochondrial heat shock protein 70 of Trypanosoma congolense and potential use as a diagnostic antigen

Clinical and Diagnostic Laboratory Immunology

Volumn 10, Issue 5, 2003, Pages 926-933

  Bannai, Hiroshi ^a   Sakurai, Tatsuya ^a   Inoue, Noboru ^a   Sugimoto, Chihiro ^a   Igarashi, Ikuo ^a  

^a OBIHIRO UNIVERSITY OF AGRICULTURE AND VETERINARY MEDICINE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; EPITOPE; HEAT SHOCK PROTEIN 70; MITOCHONDRIAL PROTEIN; MONOCLONAL ANTIBODY; PARASITE ANTIGEN; PROTEINASE; RECOMBINANT PROTEIN; RNA;

AMINO ACID SEQUENCE; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIBODY DETECTION; ANTIGEN EXPRESSION; ANTIGEN RECOGNITION; ANTIGENICITY; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; DIAGNOSTIC TEST; DNA LIBRARY; ENZYME LINKED IMMUNOSORBENT ASSAY; EXTRACTION; FEMALE; GENE; GENETIC CODE; GENOME; MOLECULAR CLONING; MOUSE; MTP GENE; NONHUMAN; NORTHERN BLOTTING; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PRIORITY JOURNAL; PROTEIN EXPRESSION; SCREENING TEST; SERODIAGNOSIS; SOUTHERN BLOTTING; TRYPANOSOMA CONGOLENSE; TRYPANOSOMIASIS; WESTERN BLOTTING; AFRICAN TRYPANOSOMIASIS; ANIMAL; COMPARATIVE STUDY; EPITOPE MAPPING; GENE LIBRARY; GENETICS; HUMAN; IMMUNOLOGY; MOLECULAR GENETICS; MOUSE MUTANT;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, MONOCLONAL; ANTIGENS, PROTOZOAN; BLOTTING, NORTHERN; BLOTTING, SOUTHERN; BLOTTING, WESTERN; CLONING, MOLECULAR; ENZYME-LINKED IMMUNOSORBENT ASSAY; EPITOPE MAPPING; FEMALE; GENE LIBRARY; HSP70 HEAT-SHOCK PROTEINS; HUMANS; MICE; MICE, KNOCKOUT; MOLECULAR SEQUENCE DATA; TRYPANOSOMA CONGOLENSE; TRYPANOSOMIASIS, AFRICAN;

EID: 0141563816     PISSN: 1071412X     EISSN: None     Source Type: Journal    
DOI: 10.1128/CDLI.10.5.926-933.2003     Document Type: Article

References (18)

1. Boulange, A., J. Katende, and E. Anthie. 2002. Trypanosoma congolense: expression of a heat shock protein 70 and initial evaluation as a diagnostic antigen for bovine trypanosomosis. Exp. Parasitol. 100:6-11.
2. Chen, M., F. Aosai, H. S. Mun, K. Norose, H. Hata, and A. Yano. 2000. Anti-HSP70 autoantibody formation by B-1 cells in Toxoplasma gondii-infected mice. Infect. Immun. 68:4893-4899.
3. Chen, M., F. Aosai, K. Norose, H. S. Mun, O. Takeuchi, S. Akira, and A. Yano. 2002. Involvement of MyD88 in host defense and the down-regulation of anti-heat shock protein 70 autoantibody formation by MyD88 in Toxoplasma gondii-infected mice. J. Parasitol. 88:1017-1019.
4. Chi, T. B., V. Silvia, B. Brown, and N. Williams. 1998. Subunit 9 of the mitochondrial ATP synthase of Trypanosoma brucei is nuclearly encoded and developmentally regulated. Mol. Biochem. Parasitol. 92:29-38.
5. Domanico, S. Z., D. C. DeNagel, J. N. Dahlseid, J. M. Green, and S. K. Pierce. 1993. Cloning of the gene encoding peptide-binding protein 74 shows that it is a new member of the heat shock protein 70 family. Mol. Cell. Biol. 13:3598-3610.
6. Engman, D. M., E. A. Dragon, and J. E. Donelson. 1990. Human humoral immunity to hsp70 during Trypanosoma cruzi infection. J. Immunol. 144:3987-3991.
7. Engman, D. M., S. Fehr, and J. E. Donelson. 1992. Specific functional domains of mitochondrial hsp70s suggested by sequence comparison of the trypanosome and yeast proteins. Mol. Biochem. Parasitol. 51:153-156.
8. Greiner, M., S. Kumar, and C. Kyeswa. 1997. Evaluation and comparison of antibody ELISAs for serodiagnosis of bovine trypanosomosis. Vet. Parasitol. 73:197-205.
9. Hausler, T., Y. D. Stierhof, J. Blattner, and C. Clayton. 1997. Conservation of mitochondrial targeting sequence function in mitochondrial and hydrogenosomal proteins from the early-branching eukaryotes Crithidia, Trypanosoma and Trichomonas. Eur. J. Cell Biol. 73:240-251.
10. Hirumi, H., and K. Hirumi. 1991. In vitro cultivation of Trypanosoma congolense bloodstream forms in the absence of feeder cell layer. Parasitology 102:225-236.
11. Inoue, N., A. T. Lluz, T. Mori, H. Nagasawa, K. Fujisaki, and T. Mikami. 2000. Novel species specific antigens of Trypanosoma congolense and their different localization among life-cycle stages. J. Vet. Med. Sci. 62:1041-1045.
12. Inoue, N., K. Otsu, D. M. Ferraro, and J. E. Donelson. 2002. Tetracycline-regulated RNA interference in Trypanosoma congolense. Mol. Biochem. Parasitol. 120:309-313.
13. LaCount, D. J., S. Bruse, K. L. Hill, and J. E. Donelson. 2000. Double-strand RNA interference in Trypanosoma brucei using head-to-head promoters. Mol. Biochem. Parasitol. 111:67-76.
14. Lanham, S. M., and D. G. Godfrey. 1970. Isolation of salivarian trypanosomes from man and other mammals using DEAE-cellulose. Exp. Parasitol. 28:521-534.
15. Polla, B. S. 1991. Heat shock proteins in host-parasite interactions. Immunol. Today 12:A38-A41.
16. Requena, J. M., A. Jimenez-Ruiz, M. Soto, R. Assiego, J. F. Santaren, M. C. Lopez, M. E. Patarroyo, and C. Alonso. 1992. Regulation of hsp70 expression in Trypanosoma cruzi by temperature and growth phase. Mol. Biochem. Parasitol. 53:201-211.
17. Searle, S., M. V. McCrossan, and D. F. Smith. 1993. Expression of mitochondrial stress protein in the protozoan parasite Leishmania major. J. Cell Sci. 104:1091-1100.
18. Silvia, V., B. Brown, T. B. Chi, and N. Williams. 2001. The Trypanosoma brucei mitochondrial ATP synthase is developmentally regulated at the level of transcript stability. Mol. Biochem. Parasitol. 115:177-187.