An efficient method for precise gene substitution in the AcMNPV genome by homologous recombination in E. coli

Journal of Virological Methods

Volumn 113, Issue 2, 2003, Pages 95-101

  Wu, Wuwei ^a   Wang, Jinwen ^a   Deng, Riqiang ^a   Wang, Xunzhang ^a   He, XiongLei ^a   Long, Qingxin ^a  

^a SUN YAT SEN UNIVERSITY   (China)

Author keywords

Baculovirus; Gene substitution; p74 gene; RecA mediated homologous recombination

Indexed keywords

PRIMER DNA;

ARTICLE; AUTOGRAPHA CALIFORNICA; BACULOVIRUS EXPRESSION SYSTEM; ESCHERICHIA COLI; GENE CONTROL; GENE EXPRESSION REGULATION; GENE FUNCTION; GENE INSERTION; GENETIC TRANSCRIPTION; GENETIC TRANSFECTION; HOMOLOGOUS RECOMBINATION; NONHUMAN; PLASMID; POLYHEDROSIS VIRUS; PRIORITY JOURNAL; PROMOTER REGION; RECA GENE; RESEARCH; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SPODOPTERA LITURA; VIRUS GENE; VIRUS GENOME;

AUTOGRAPHA; AUTOGRAPHA CALIFORNICA; AUTOGRAPHA CALIFORNICA NUCLEOPOLYHEDROVIRUS; ESCHERICHIA COLI; NUCLEOPOLYHEDROVIRUS; SPODOPTERA; SPODOPTERA LITURA; SPODOPTERA LITURA MULTICAPSID NUCLEOPOLYHEDROVIRUS; UNIDENTIFIED BACULOVIRUS;

EID: 0141560444     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(03)00225-8     Document Type: Article

References (20)

1. Bideshi D.K., Federici B.A. The Trichoplusia ni granulovirus helicase is unable to support replication of Autographa californica multicapsid nucleopolyhedrovirus in cells and larvae of T. ni. J. Gen. Virol. 81:2000;1593-1599.
2. Court D.L., Sawitzke J.A., Thomason L.C. Genetic engineering using homologous recombination. Annu. Rev. Genet. 36:2002;361-388.
3. Deng X., Zhu Y., Diao Z., Qi Y., Zhou Z. Construction of a novel Bm NPV Bac to Bac system. Acta Microbiol. Sin. 40:2000;155-160.
4. Faulkner P., Kuzio J., Williams G.V., Wilson J.A. Analysis of p74, a PDV envelope protein of Autographa californica multicapsid nucleopolyhedrovirus required for occlusion body infectivity in vivo. J. Gen. Virol. 78:1997;3091-3100.
5. Hou S., Chen X., Wang H., Tao M., Hu Z. Efficient method to generate homologous recombinant baculovirus genomes in E. coli. Biotechniques. 32(4):2002;783-788.
6. Jones I., Morikawa Y. Baculovirus vectors for expression in insect cells. Curr. Opin. Biotechnol. 7(5):1996;512-516.
7. Kitts P.A., Possee R.D. A method for producing recombinant baculovirus expression vectors at high frequency. Biotechniques. 14(5):1993;810-817.
8. Lalioti M., Heath J. A new method for generating point mutations in bacterial artificial chromosomes by homologous recombination in Escherichia coli. Nucleic Acids Res. 29(3):2001;E14.
9. Lin G., Blissard G.W. Analysis of an Autographa californica nucleopolyhedrovirus lef-11 knockout: LEF-11 is essential for viral DNA replication. J. Virol. 76(6):2002;2770-2779.
10. Lu A., Miller L.K. Generation of recombinant baculoviruses by direct cloning. Biotechniques. 21(1):1996;63-68.
11. Luckow V.A., Lee S.C., Barry G.F., Olins P.O. Efficient generation of infectious recombinant baculoviruses by site-specific transposon-mediated insertion of foreign genes into a baculovirus genome propagated in Escherichia coli. J. Virol. 67:1993;4566-4579.
12. Nefedov, M., Williamson, R., Ioannou, P.A., 2000. Insertion of disease-causing mutations in BACs by homologous recombination in Escherichia coli. Nucleic Acids Res. 28 (17) E79.
13. Pang Y., Yu J., Wang L., Hu X., Bao W., Li G., Chen C.et al. Sequence analysis of the Spodoptera litura multicapsid nucleopolyhedrovirus genome. Virology. 287(2):2001;391-404.
14. Patel G., Nasmyth K., Jones N. A new method for the isolation of recombinant baculovirus. Nucleic Acids Res. 20(1):1992;97-104.
15. Peakman T.C., Harris R.A., Gewert D.R. Highly efficient generation of recombinant baculoviruses by enzymatically medicated site-specific in vitro recombination. Nucleic Acids Res. 20(3):1992;495-500.
16. Pijlman G.P., Dortmans J.C., Vermeesch A.M., Yang K., Martens D.E., Goldbach R.W., Vlak J.M. Pivotal role of the non-hr origin of DNA replication in the genesis of defective interfering baculoviruses. J. Virol. 76(11):2002;5605-5611.
17. Shizuya H., Birren B., Kim U.J., Mancino V., Slepak T., Tachiiri Y., Simon M. Cloning and stable maintenance of 300 kilobase pair fragments of human DNA in Escherichia coli using an F factor based vector. Proc. Natl. Acad. Sci. U.S.A. 89:1992;8794-8797.
18. Yang X.W., Model P., Heintz N. Homologous recombination based modification in Escherichia coli and germline transmission in transgenic mice of a bacterial artificial chromosome. Nat. Biotechnol. 15:1997;859-865.
19. Zhang Y., Buchholz F., Muyrers J.P., Stewart A.F. A new logic for DNA engineering using recombination in Escherichia coli. Nat. Genet. 20(2):1998;123-128.
20. Zhu Y., Qi Y.P., Liu D.L. Construction of a shuttle vector using HaNPV and expression of GFP gene in insect cell and larvae. J. Wuhan Univ. (Nat. Sci. Ed.). 43(6):1997;819-820.