Kinetic studies on endo-β-galactosidase by a novel colorimetric assay and synthesis of N-acetyllactosamine-repeating oligosaccharide β-glycosides using its transglycosylation activity

European Journal of Biochemistry

Volumn 270, Issue 18, 2003, Pages 3709-3719

  Murata, Takeomi ^a   Hattori, Takeshi ^a   Amarume, Satoshi ^a   Koichi, Akiko ^a   Usui, Taichi ^a  

^a SHIZUOKA UNIVERSITY   (Japan)

Author keywords

Endo galactosidase; Enzyme assay; Kinetics; Poly (N acetyllactosamine); Transglycosylation

Indexed keywords

4 NITROPHENOL; BETA GALACTOSIDASE; BETA GLUCOSIDASE; BETA1 GALACTOSAMINE BETA1 N ACETYL 3 GLUCOSAMINE BETA1 3 GALACTOSAMINE BETA 4 GLUCOSAMINE 4 NITROPHENYL; BETA1 GALACTOSAMINE BETA1 N ACETYL 4 GLUCOSAMINE BETA1 3 GALACTOSAMINE BETA 4 GLUCOSAMINE 4 NITROPHENYL; BETA1 GALACTOSAMINE BETA1 N ACETYL 4 GLUCOSAMINE BETA1 3 GALACTOSAMINE BETA N ACETYL 4 GLUCOSAMINE 4 NITROPHENYL; BETA1 GALACTOSAMINE BETA1 N ACETYL 6 GLUCOSAMINE BETA1 3 GALACTOSAMINE BETA 4 GLUCOSAMINE 4 NITROPHENYL; BETA1 N ACETYLGLUCOSAMINE BETA1 3 GALACTOSAMIDE BETA1 N ACETYL 4 GLUCOSAMINE BETA1 3 GALACTOSAMINE BETA N ACETYL 4 GLUCOSAMINE 4 NITROPHENYL; BETA1 N ACETYLGLUCOSAMINE BETA1 3 GALACTOSAMINE BETA 4 GLUCOSAMINE 4 NITROPHENYL; BETA1 N ACETYLGLUCOSAMINE BETA1 3 GALACTOSAMINE BETA N ACETYL 4 GLUCOSAMINE 4 NITROPHENYL; BETA1 N ACETYLGLUCOSAMINE BETA1 3 GALACTOSAMINE BETA1 N ACETYL 4 GLUCOSAMINE BETA1 3 GALACTOSAMINE BETA1 N ACETYL 4 GLUCOSAMINE BETA1 3 GALACTOSAMINE BETA N ACETYL 4 GLUCOSAMINE 4 NITROPHENYL; HYDROLASE; KERATAN SULFATE; N ACETYLLACTOSAMINE SYNTHASE; OLIGOSACCHARIDE; UNCLASSIFIED DRUG;

ARTICLE; CARBOHYDRATE SYNTHESIS; CATALYSIS; CITROBACTER FREUNDII; COLORIMETRY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME GLYCOSYLATION; ENZYME KINETICS; ENZYME SUBSTRATE; HYDROLYSIS; KINETICS; NONHUMAN; PRIORITY JOURNAL;

AMINO SUGARS; BACTEROIDES FRAGILIS; BETA-GALACTOSIDASE; BINDING SITES; CALORIMETRY; CARBOHYDRATE SEQUENCE; CITROBACTER FREUNDII; GLYCOSIDE HYDROLASES; GLYCOSIDES; GLYCOSYLATION; HYDROLYSIS; ISOMERISM; KERATAN SULFATE; KINETICS; MOLECULAR SEQUENCE DATA; NITROPHENOLS; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; OLIGOSACCHARIDES; SUBSTRATE SPECIFICITY;

CITROBACTER; CITROBACTER FREUNDII; ESCHERICHIA;

EID: 0141558968     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1033.2003.03757.x     Document Type: Article

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