A molecular throttle: The recombination hotspot χ controls DNA translocation by the RecBCD helicase

Cell

Volumn 114, Issue 5, 2003, Pages 647-654

  Spies, Maria ^a   Bianco, Piero R ^a,b   Dillingham, Mark S ^a,c   Handa, Naofumi ^a   Baskin, Ronald J ^a   Kowalczykowski, Stephen C ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY AT BUFFALO   (United States)

^c NATIONAL INSTITUTE FOR MEDICAL RESEARCH   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CARRIER PROTEIN; DNA; ENZYME; HELICASE; HOLOENZYME; PROTEIN SUBUNIT; RECBCD HELICASE; UNCLASSIFIED DRUG;

ARTICLE; DNA SEQUENCE; ENZYME ACTIVITY; GENE TRANSLOCATION; MOLECULAR MECHANICS; MOLECULE; PRIORITY JOURNAL; PROTEIN INTERACTION;

EID: 0141540814     PISSN: 00928674     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0092-8674(03)00681-0     Document Type: Article

References (30)

1. Anderson D.G., Kowalczykowski S.C. The recombination hot spot χ is a regulatory element that switches the polarity of DNA degradation by the RecBCD enzyme. Genes Dev. 11:1997;571-581. a.
2. Anderson D.G., Kowalczykowski S.C. The translocating RecBCD enzyme stimulates recombination by directing RecA protein onto ssDNA in a χ-regulated manner. Cell. 90:1997;77-86. b.
3. Anderson D.G., Churchill J.J., Kowalczykowski S.C. χ-activated RecBCD enzyme possesses 5′→3′ nucleolytic activity, but RecBC enzyme does not. evidence suggesting that the alteration induced by χ is not simply ejection of the RecD subunit Genes Cells. 2:1997;117-128.
4. Anderson D.G., Churchill J.J., Kowalczykowski S.C. A single mutation, RecB(D1080A), eliminates RecA protein loading but not χ recognition by RecBCD enzyme. J. Biol. Chem. 274:1999;27139-27144.
5. Arnold, D.A., and Kowalczykowski, S.C. (1999). RecBCD helicase/nuclease. In Encyclopedia of Life Sciences (London: Nature Publishing Group).
6. 1004D enzyme reflect its recombination defect in vivo. J. Biol. Chem. 273:1998;16476-16486.
7. Bianco P.R., Kowalczykowski S.C. The recombination hotspot χ is recognized by the translocating RecBCD enzyme as the single strand of DNA containing the sequence 5′-GCTGGTGG-3′ Proc. Natl. Acad. Sci. USA. 94:1997;6706-6711.
8. Bianco P.R., Brewer L.R., Corzett M., Balhorn R., Yeh Y., Kowalczykowski S.C., Baskin R.J. Processive translocation and DNA unwinding by individual RecBCD enzyme molecules. Nature. 409:2001;374-378.
9. Dillingham M.S., Spies M., Kowalczykowski S.C. RecBCD enzyme is a bipolar DNA helicase. Nature. 423:2003;893-897.
10. Dixon D.A., Kowalczykowski S.C. Homologous pairing in vitro stimulated by the recombination hotspot, χ Cell. 66:1991;361-371.
11. Dixon D.A., Kowalczykowski S.C. The recombination hotspot χ is a regulatory sequence that acts by attenuating the nuclease activity of the E. coli RecBCD enzyme. Cell. 73:1993;87-96.
12. Dixon D.A., Kowalczykowski S.C. Role of the Escherichia coli recombination hotspot, χ, in RecABCD-dependent homologous pairing. J. Biol. Chem. 270:1995;16360-16370.
13. Dixon D.A., Churchill J.J., Kowalczykowski S.C. Reversible inactivation of the Escherichia coli RecBCD enzyme by the recombination hotspot χ in vitro. evidence for functional inactivation or loss of the RecD subunit Proc. Natl. Acad. Sci. USA. 91:1994;2980-2984.
14. Dohoney K.M., Gelles J. χ-sequence recognition and DNA translocation by single RecBCD helicase/nuclease molecules. Nature. 409:2001;370-374.
15. Korangy F., Julin D.A. Efficiency of ATP hydrolysis and DNA unwinding by the RecBC enzyme from Escherichia coli. Biochemistry. 33:1994;9552-9560.
16. Kuzminov A., Schabtach E., Stahl F.W. χ sites in combination with RecA protein increase the survival of linear DNA in Escherichia coli by inactivating exoV activity of RecBCD nuclease. EMBO J. 13:1994;2764-2776.
17. Lam S.T., Stahl M.M., McMilin K.D., Stahl F.W. Rec-mediated recombinational hot spot activity in bacteriophage λ. II. A mutation which causes hot spot activity. Genetics. 77:1974;425-433.
18. Larsson A., Carlsson C., Jonsson M., Albisson B. Characterization of the binding of the fluorescent dyes YO and YOYO to DNA by polarized light spectroscopy. J. Am. Chem. Soc. 116:1994;8459-8465.
19. LeBowitz, J. (1985). Biochemical mechanism of strand initiation in bacteriophage λ DNA replication, PhD thesis, Johns Hopkins University, Baltimore, Maryland.
20. Myers R.S., Kuzminov A., Stahl F.W. The recombination hot spot χ activates RecBCD recombination by converting Escherichia coli to a recD mutant phenocopy. Proc. Natl. Acad. Sci. USA. 92:1995;6244-6248.
21. Rinken R., Thomas B., Wackernagel W. Evidence that recBC-dependent degradation of duplex DNA in Escherichia coli recD mutants involves DNA unwinding. J. Bacteriol. 174:1992;5424-5429.
22. Roman L.J., Kowalczykowski S.C. Characterization of the helicase activity of the Escherichia coli RecBCD enzyme using a novel helicase assay. Biochemistry. 28:1989;2863-2873.
23. Roman L.J., Eggleston A.K., Kowalczykowski S.C. Processivity of the DNA helicase activity of Escherichia coli recBCD enzyme. J. Biol. Chem. 267:1992;4207-4214.
24. Stahl F.W., Stahl M.M. Rec-mediated recombinational hot spot activity in bacteriophage λ. IV. Effect of heterology on χ-stimulated crossing over. Mol. Gen. Genet. 140:1975;29-37.
25. Stahl F.W., Crasemann J.M., Stahl M.M. Rec-mediated recombinational hot spot activity in bacteriophage λ. III. χ mutations are site-mutations stimulating rec-mediated recombination. J. Mol. Biol. 94:1975;203-212.
26. Taylor A., Smith G.R. Unwinding and rewinding of DNA by the recBC enzyme. Cell. 22:1980;447-457.
27. Taylor A.F., Smith G.R. RecBCD enzyme is altered upon cutting DNA at a χ recombination hotspot. Proc. Natl. Acad. Sci. USA. 89:1992;5226-5230.
28. Taylor A.F., Smith G.R. Regulation of homologous recombination. χ inactivates RecBCD enzyme by disassembly of the three subunits Genes Dev. 13:1999;890-900.
29. Taylor A.F., Smith G.R. RecBCD enzyme. a DNA helicase with fast and slow motors Nature. 423:2003;889-893.
30. Taylor A.F., Schultz D.W., Ponticelli A.S., Smith G.R. RecBC enzyme nicking at χ sites during DNA unwinding. location and orientation-dependence of the cutting Cell. 41:1985;153-163.