Rapid detection and quantification of cell free cytomegalovirus by a high-speed centrifugation-based microculture assay: Comparison to longitudinally analyzed viral DNA load and pp67 late transcript during lactation

Journal of Clinical Virology

Volumn 28, Issue 3, 2003, Pages 303-316

  Hamprecht, Klaus ^a   Witzel, Simone ^a   Maschmann, Jens ^b   Dietz, Klaus ^a   Baumeister, Andrea ^a   Mikeler, Elfriede ^a   Goelz, Rangmar ^a   Speer, Christian P ^b   Jahn, Gerhard ^a  

^a UNIVERSITY HOSPITAL TÜBINGEN   (Germany)

^b UNIVERSITY OF WÜRZBURG   (Germany)

Author keywords

Breast milk; Breastfeeding; CMV; Microculture; NASBA; Quantitative PCR

Indexed keywords

MESSENGER RNA; MILK FAT; MILK PROTEIN; PROTEIN PP 67; UNCLASSIFIED DRUG; VIRUS DNA; VIRUS PROTEIN;

ANALYTIC METHOD; ARTICLE; BREAST FEEDING; CELL; CELL CULTURE; CENTRIFUGATION; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DNA DETERMINATION; DNA TRANSCRIPTION; DYNAMICS; EXTRACTION; FEMALE; HUMAN; HUMAN CYTOMEGALOVIRUS; INCIDENCE; INOCULATION; INTERMETHOD COMPARISON; LACTATION; LONGITUDINAL STUDY; MILK; NEWBORN; POLYMERASE CHAIN REACTION; PREMATURITY; PRIORITY JOURNAL; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; RNA ANALYSIS; SAMPLE; SCREENING; SENSITIVITY ANALYSIS; SEPARATION TECHNIQUE; SEROLOGY; TECHNIQUE; TISSUE CULTURE; VELOCITY; VIRUS DETECTION; VIRUS LOAD; VIRUS PARTICLE; VIRUS REACTIVATION; VIRUS TRANSMISSION;

EID: 0141540513     PISSN: 13866532     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1386-6532(03)00074-X     Document Type: Article

References (31)

1. American Academy of Pediatrics, Workgroup on Breastfeeding. Breastfeeding and the use of human milk (RE9729), Pediatrics 1997;100:1035-39.
2. Asanuma H., Numazaki K., Nagata N., Hotsubo T., Horino K., Chiba S. Role of milk whey in the transmission of human cytomegalovirus infection by breast milk. Microbiol. Immunol. 40:1996;201-204. Blok M.J., Goossens V.J., Vanherle S.J., Top B., Tacken N., Middeldorp J.M., Christiaans M.H., van Hooff J.P., Bruggeman C.A. Diagnostic value of monitoring human cytomegalovirus late pp67 mRNA expression in renal-allograft recipients by nucleic acid sequence-based amplification. J. Clin. Microbiol. 36:1998;1341-1346.
3. Blok M.J., Goossens V.J., Vanherle S.J., Top B., Tacken N., Middeldorp J.M., Christiaans M.H., van Hooff J.P., Bruggeman C.A. Diagnostic value of monitoring human cytomegalovirus late pp67 mRNA expression in renal-allograft recipients by nucleic acid sequence-based amplification. J. Clin. Microbiol. 36:1998;1341-1346.
4. Boom R.C., Sol J.A., Salimans M.M.M., Jansen C.L., Wertheim-van Dillen P.M.E., van der Nordaa J. Rapid and simple method for purification of nucleic acids. J. Clin. Microbiol. 28:1990;495-503.
5. Bryant P., Morley C., Garland S., Curtis N. Cytomegalovirus transmission from breast milk in premature babies: does it matter? Arch. Dis. Child. Fetal Neonatal Ed. 87:2002;F75-F77.
6. Davis M.G., Huang E.-S. Nucleotide sequence of a human cytomegalovirus DNA fragment encoding a 67-kDa phosphorylated viral protein. J. Virol. 56:1985;7-11.
7. Dworsky M., Yow M., Stagno S., Pass R.F., Alford C. Cytomegalovirus infection of breast milk and transmission in infancy. Pediatrics. 72:1983;295-299.
8. Emery V.C., Cope A.V., Bowen E.F., Gor D., Griffiths P.D. The dynamics of human cytomegalovirus replication in vivo. J. Exp. Med. 190:1999;177-182.
9. Gerna G., Baldanti F., Middeldorp J., Furione M., Zavattoni M., Lilleri D., Revello M.G. Clinical significance of expression of human cytomegalovirus pp67 late transcript in heart, lung, and bone marrow transplant recipients as determined by nucleic acid sequence-based amplification. J. Clin. Microbiol. 37:1999;902-911.
10. Gleaves C.A., Smith T.F., Shuster E.A., Pearson G.R. Rapid detection of cytomegalovirus in MRC-5 cells inoculated with urine specimens by using low-speed centrifugation and monoclonal antibody to an early antigen. J. Clin. Microbiol. 19:1984;917-919.
11. Hamprecht K., Steinmaβl M., Einsele H., Jahn G. Discordant detection of human cytomegalovirus DNA from peripheral blood mononuclear cells, granulocytes and plasma: correlation to viremia and HCMV infection. J. Clin. Virol. 11:1998;125-136.
12. Hamprecht K., Vochem M., Baumeister A., Boniek M., Speer C.P., Jahn G. Detection of cytomegaloviral DNA in human milk cells and cell free milk whey by nested PCR. J. Virol. Methods. 70:1998;167-176.
13. Hamprecht K, Witzel S, Maschmann J, Speer CP, Jahn G. Transmission of cytomegalovirus infection through breast milk in term and preterm infants. The role of cell free milk whey and milk cells. In: Koletzko B, Hernell O, Michaelsen KF, editors. Short and Long Term Effects of Breast Feeding on Child Health. London: Kluwer Academic, Plenum Press. Adv. Exp. Med. Biol. 2000;4 78:231-9.
14. Hamprecht K., Maschmann J., Vochem M., Dietz K., Speer C.P., Jahn G. Epidemiology of transmission of cytomegalovirus from mother to preterm infant by breastfeeding. Lancet. 357:2001;513-518.
15. Hayes K., Danks D.M., Gibas H., Jack I. Cytomegalovirus in human milk. New Engl. J. Med. 27:1972;177-178.
16. Hebart H., Rudolph T., Loeffler J., Middeldorp J., Ljubicic T., Jahn G., Einsele H. Evaluation of the NucliSens CMV pp67 assay for detection and monitoring of human cytomegalovirus infection after allogeneic stem cell transplantation. Bone Marrow Transplant. 30:2002;181-187.
17. Hotsubo T., Nagata N., Shimada M., Yoshida K., Fujinaga K., Chiba S. Detection of human cytomegalovirus DNA in breast milk by means of polymerase chain reaction. Microbiol. Immunol. 38:1994;809-811.
18. Jabs D.A., Forman M., Enger C., Jackson J.B. Comparison of cytomegalovirus loads in plasma and leukocytes of patients with cytomegalovirus retinitis. The cytomegalovirus retinitis and viral resistance study group. J. Clin. Microbiol. 37:1999;1431-1435.
19. Maschmann J., Hamprecht K., Dietz K., Jahn G., Speer C.P. Cytomegalovirus infection of extremely low-birth weight infants via breast milk. Clin. Infect. Dis. 33:2001;1998-2003.
20. Middeldorp J.M., Sillekens P., Lunenberg J. Diagnosis of active HCMV infection: the mRNA approach. Organs Tissues. 2:2000;99-107.
21. Schäfer P., Braun R.W., Möhring K., Henco K., Kang J., Wendland T., Kühn J.E. Quantitative determination of human cytomegalovirus target sequences in peripheral blood leucocytes by nested polymerase chain reaction and temperature gradient gel electrophoresis. J. Gen. Virol. 74:1993;2699-2707.
22. Schäfer P., Tenschert W., Cremaschi L., Schröter M., Zöllner B., Laufs R. Area under the viraemia curve versus absolute viral load: utility for predicting symptomatic cytomegalovirus infections in kidney transplant patients. J. Med. Virol. 65:2001;85-89.
23. Stagno S., Cloud G.A. Working parents: the impact of day care and breast-feeding on cytomegalovirus infections in offspring. Proc. Natl. Acad. Sci. USA. 91:1994;2384-2389.
24. Stagno S., Reynolds D.W., Pass R.F., Alford C.A. Breast milk and the risk of cytomegalovirus infection. New Engl. J. Med. 302:1980;1073-1076.
25. Stirk P.R., Griffiths P.D. Use of monoclonal antibodies for the diagnosis of cytomegalovirus infection by the detection of early antigen fluorescent foci (DEAFF) in cell culture. J. Med. Virol. 21:1987;329-337.
26. Storch G.A., Buller R.S., Bailey T.C., Ettinger N.A., Langlois T., Gaudreault-Keener M., Welby P.L. Comparison of PCR and pp65 antigenemia assay with quantitative shell vial culture for detection of cytomegalovirus in blood leukocytes from solid-organ transplant recipients. J. Clin. Microbiol. 32:1994;997-1003.
27. Tong C.Y., Cuevas L.E., Williams H., Bakran A. Comparison of two commercial methods for measurement of cytomegalovirus load in blood samples after renal transplantation. J. Clin. Microbiol. 38:2000;209-1213.
28. Topilko A., Michelson S. Morphological and cytochemical analysis of human cytomegalovirus inoculum: correlation of free particles in inoculum with counterparts in infected cells. Res. Virol. 145:1994;65-73.
29. Van Der Strate B.W.A., Harmsen M.C., Schäfer P., Swart P.J., The T.H., Jahn G., Speer C.P., Meijer D.K.F., Hamprecht K. Viral load in breast milk correlates with transmission of human cytomegalovirus to preterm neonates, but lactoferrin concentrations do not. Clin. Diagn. Lab. Immunol. 8:2001;818-821.
30. Warren W.P., Balcarek K., Smith R., Pass R.F. Comparison of rapid methods of detection of cytomegalovirus in saliva with virus isolation in tissue culture. J. Clin. Microbiol. 30:1992;786-790.
31. Xanthou M., Bines J., Walker W.A. Human milk and intestinal host defense in newborns: an update. Adv. Pediatr. 42:1995;171-208.