In vitro and in situ experimental model for x-ray microanalysis of intestinal epithelium

Microscopy Research and Technique

Volumn 62, Issue 3, 2003, Pages 211-217

  Vanthanouvong, Viengphet ^a   Högman, Marieann ^a   Roomans, Godfried M ^a  

^a UPPSALA UNIVERSITY   (Sweden)

Author keywords

In situ; In vitro; Intestine; Ion transport; X ray microanalysis

Indexed keywords

CELLS; CHLORINE COMPOUNDS; CYTOLOGY; IONS; SODIUM;

EXPERIMENTAL MODELLING; IN SITU; IN-VITRO; INTESTINAL EPITHELIAL CELLS; INTESTINAL EPITHELIUM; INTESTINE; ION-TRANSPORT; PERFUSION FLUID; SITU SYSTEM; X-RAY MICROANALYSIS;

GLUCOSE;

BROMINE; CHLORIDE ION; POTASSIUM ION; SODIUM ION;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CHLORIDE TRANSPORT; IMAGE ANALYSIS; INTESTINE CRYPT; INTESTINE VILLUS; MALE; MOUSE; NONHUMAN; PRIORITY JOURNAL; RAT; SMALL INTESTINE MUCOSA; TRANSPORT KINETICS; X RAY MICROANALYSIS;

ANIMALIA;

EID: 0141540470     PISSN: 1059910X     EISSN: None     Source Type: Journal    
DOI: 10.1002/jemt.10359     Document Type: Article

References (17)

1. Ashcroft FM. 2000. Ion channels and disease. San Diego: Academic Press.
2. Cuthbert AW, MacVinish LJ, Hickman ME, Ratcliff R, Colledge WH, Evans MJ. 1994. Ion-transporting activity in the murine colonic epithelium of normal animals and animals with cystic fibrosis. Pflügers Arch 428:508-515.
3. Dragomir A, Roomans GM. 2000. X-ray microanalysis of intestinal chloride transport in vitro and in vivo. Microsc Res Tech 50:176-181.
4. Fedan JS, Frazer DG. 1992. Influence of epithelium on the reactivity of guinea pig isolated, perfused trachea to bronchoactive drugs. J Pharmacol Exp Ther 262:741-750.
5. Gray MA, Plant S, Argent BA. 1993. cAMP-regulated whole cell chloride currents in pancreatic duct cells. Am J Physiol 264:C591-C602.
6. Grubb BR, Boucher RC. 1999. Pathophysiology of gene-targeted mouse models for cystic fibrosis. Physiol Rev 79(Suppl):S193-214.
7. Grubb BR, Gabriel SE. 1997. Intestinal physiology and pathology in gene-targeted mouse models of cystic fibrosis. Am J Physiol 273: G258-266.
8. Halm DR, Frizzell RA. 1992. Anion permeation in an apical membrane chloride channel of a secretory epithelial cell. J Gen Physiol 99:339-366.
9. Kawashima Y, Takeoshi I, Furukawa H, Lee RG, Starzl TE, Todo S. 1999. Cold preservation of the human colon and ileum with University of Wisconsin solution. Clin Transplant 13:420-425.
10. Kozlova I, Dragomir A, Vanthanouvong V, Roomans GM. 2000. Effects of nicotine on intestinal epithelial cells in vivo and in vitro: an X-ray microanalytical study. J Submicrosc Cytol Pathol 32:97-102.
11. McMillan EB, Roomans GM. 1990. Techniques for X-ray microanalysis of intestinal epithelium using bulk specimens. Biomed Res (India) 1:1-10.
12. Minor T, Isselhard W. 1998. Cellular signal level of cyclic AMP and functional integrity of the small bowel after ischemic preservation: an experimental pilot study in the rat. Eur Surg Res 30:144-148.
13. Munakata M, Mitzner W, Menkes H. 1988. Osmotic stimuli induce epithelial-dependent relaxation in the guinea pig trachea. J Appl Physiol 64:466-471.
14. Roomans GM. 1988. Quantitative X-ray microanalysis of biological specimens. J Electron Microsc Tech 9:19-44.
15. + conductances activated by cell swelling in primary cultures of rabbit distal bright convoluted tubules. Am J Physiol 273:F680-F697.
16. Wróblewski R, Roomans GM, Jansson E, Edström L. 1978. Electron probe X-ray microanalysis of human muscle biopsies. Histochemistry 55:281-292.
17. Wróblewski J, Sagström S, Mulders H, Roomans GM. 1989. Use of strontium and bromide as elemental tracers in X-ray microanalysis of biological tissue. Scan Microsc 3:861-864.