Fluorometric quantification of RNA and DNA in solutions containing both nucleic acids

Analytical Biochemistry

Volumn 322, Issue 1, 2003, Pages 48-50

  Morozkin, Evgeniy S ^a   Laktionov, Pavel P ^a   Rykova, Elena Y ^a   Vlassov, Valentin V ^a  

^a INSTITUTE OF CHEMICAL BIOLOGY AND FUNDAMENTAL MEDICINE   (Russian Federation)

Author keywords

DNA quantification; Hoechst 33258; RNA quantification; SYBR Green II

Indexed keywords

BIOMOLECULES; DNA;

DNA CONCENTRATION; DNA QUANTIFICATION; DNASE I; HOECHST 33258; MIXED SAMPLES; QUANTITATIVE DETERMINATIONS; RNA CONCENTRATION; SYBR GREEN II;

RNA;

ARTICLE; CALCULATION; CONTROLLED STUDY; CYTOFLUOROMETRY; DIAGNOSTIC ACCURACY; DNA CONFORMATION; DNA DETERMINATION; DNA PROBE; HUMAN; HUMAN CELL; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REPRODUCIBILITY; RNA ANALYSIS; RNA CONFORMATION; RNA PROBE;

EID: 0141535053     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2003.07.009     Document Type: Article

References (9)

1. Labarca C., Paigen K. A simple, rapid, and sensitive DNA assay procedure. Anal. Biochem. 102:1980;344-352.
2. Le Pecq J.B., Paoletti C. A new fluorometric method for RNA and DNA determination. Anal. Biochem. 17:1966;100-107.
3. Markovitz J., Roques B.P., Le Pecq J.B. Ethidium dimer: a new reagent for the fluorimetric determination of nucleic acids. Anal. Biochem. 94:1979;259-264.
4. Rye H.S., Dabora J.M., Quesada M.A., Mathies R.A., Glazer A.M. Fluorometric assay using dimeric dyes for double- and single-stranded DNA and RNA with picogram sensitivity. Anal. Biochem. 208:1993;144-150.
5. Schmidt D.M., Ernst J.D. A fluorometric assay for the quantification of RNA in solution with nanogram sensitivity. Anal. Biochem. 232:1995;144-146.
6. Jones L.J., Yue S.T., Cheung C.Y., Singer V.L. RNA quantitation by fluorescence-based solution assay: RiboGreen reagent characterization. Anal. Biochem. 265:1998;368-374.
7. Beld M., Sol C., Goudsmit J., Boom R. Fractionation of nucleic acids into single-stranded and double-stranded forms. Nucleic Acids Res. 24:1996;2618-2619.
8. Gallagher S.R. Ausubel F.M., Brent R., Kingston R.E., Moore D.D., Seidman J.G., Smith J.A., Struhl K., Albright L.M., Coen D.M., Varki A., Janssen K. Current Protocols in Molecular Biology. vol. 2:1994;A.3D.1-A.3D.8 Wiley, New York.
9. Haugland R. Handbook of Fluorescent Probes and Research Products. ninth ed. 2002;Molecular Probes, Inc. Eugene, OR.