High throughput screening protein kinase assays optimized for reaction, binding, and detection totally within a 96-well plate

Journal of Biomolecular Screening

Volumn 1, Issue 1, 1996, Pages 47-51

  Pitt, Aldo M ^a   Lee, Carolyn ^a  

^a MILLIPORE CORPORATION   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0141521944     PISSN: 10870571     EISSN: None     Source Type: Journal    
DOI: 10.1177/108705719600100115     Document Type: Article

References (14)

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7. Caverzasio, J., Bonjour, J.P. (1992). Tyrosine phosphorylation selectively regulates renal cellular phosphate transport. Evidence that it mediates the stimulatory effect of insulin-like growth factor-1. Endocrinology 130:373.
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10. Schilling, R., Morgan, D., Kilpatrick, B. (1994). A high-throughput assay for cyclic nucleotide phosphodiesterases. Anal. Biochem. 216:154.
11. Witt, J.J., Roskoski, R. (1975). Rapid protein kinase assay using phosphocellulose-paper absorption. Anal. Biochem. 66:253.
12. Glass, D.E., Masaracchia, R.A., Feramisco, J.R., Kemp, B.E. (1978). Isolation of phosphorylated peptides and proteins on ion exchange papers. Anal. Biochem. 87:566.
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14. Pitt, A.M., Gabriels, J.E. (1996). Characterization of epidermal growth factor binding and receptor tyrosine kinase activity of A431 cells using a filtration based 96-well assay system. Poster pre-sented at the Ninth International Conference on Second Messengers And Phosphoproteins, Nashville, TN, October 31, 1995. Abstract # 614, in Advances in Second Messengers and Phosphoprotein Research, Raven Press, New York.