Control of enzyme IIscr and sucrose-6-phosphate hydrolase activities in Streptococcus mutans by transcriptional repressor ScrR binding to the cis-active determinants of the scr regulon

Journal of Bacteriology

Volumn 185, Issue 19, 2003, Pages 5791-5799

  Wang, Bing ^a   Kuramitsu, Howard K ^a  

^a UNIVERSITY AT BUFFALO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; CIS ACTING ELEMENT; DEOXYRIBONUCLEASE I; GENE PRODUCT; HYBRID PROTEIN; PROTEIN SCRR; SCR ENZYME II; SUCROSE 6 PHOSPHATE HYDROLASE; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; BETA FRUCTOFURANOSIDASE; GLYCOSIDASE; PHOSPHOENOLPYRUVATE SUCROSE PHOSPHOTRANSFERASE; PHOSPHOENOLPYRUVATE SUGAR PHOSPHOTRANSFERASE; PHOSPHOENOLPYRUVATE-SUCROSE PHOSPHOTRANSFERASE; REPRESSOR PROTEIN; SCRR PROTEIN, BACTERIA;

ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BINDING AFFINITY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME MECHANISM; GEL MOBILITY SHIFT ASSAY; GENE EXPRESSION; GENE IDENTIFICATION; GENE MUTATION; GENE SEQUENCE; GENETIC CODE; GLUCOSE TRANSPORT; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN BINDING; PROTEIN DNA BINDING; REGULON; SCR GENE; SCRA GENE; SCRB GENE; STREPTOCOCCUS MUTANS; TRANSCRIPTION REGULATION; TRANSCRIPTION TERMINATION; AMINO ACID SEQUENCE; CHEMISTRY; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; OPERATOR GENE; PHYSIOLOGY;

BACTERIA (MICROORGANISMS); POSIBACTERIA; STREPTOCOCCUS; STREPTOCOCCUS MUTANS;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BASE SEQUENCE; BETA-FRUCTOFURANOSIDASE; GENE EXPRESSION REGULATION, BACTERIAL; GLYCOSIDE HYDROLASES; MOLECULAR SEQUENCE DATA; OPERATOR REGIONS (GENETICS); PHOSPHOENOLPYRUVATE SUGAR PHOSPHOTRANSFERASE SYSTEM; PROMOTER REGIONS (GENETICS); REGULON; REPRESSOR PROTEINS; STREPTOCOCCUS MUTANS;

EID: 0141504199     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.185.19.5791-5799.2003     Document Type: Article

References (24)

1. Ausubel, F. M., R. Brent, R. Kingston, D. D. Moore, J. G. Seldman, J. A. Smith, and K. Struhl. 1994. Curent protocols in molecular biology. John Wiley & Sons, Inc., New York, N.Y.
2. Baev, D., R. England, and H. K. Kuramitsu. 1999. Stress-induced membrane association of the Streptococcus mutans GTP-binding protein, an essential G protein, and investigation of its physiological role by utilizing an antisense RNA strategy. Infect. Immun. 67:4510-4516.
3. Chassy, B. M., and E. V. Porter. 1979. Initial characterization of sucrose-6-phosphate hydrolase from Streptococcus mutans and its apparent identity with intracellular invertase. Biochem. Biophys. Res. Commun. 89:307-314.
4. Debarbouille, M., M. Arnaud, A. Fouet, A. Klier, and G. Rapoport. 1990. The sacT gene regulating the sacPA operon in Bacillus subtilis shares strong homology with transcriptional antiterminators. J. Bacteriol. 172:3966-3973.
5. Gering, M., and R. Bruckner. 1996. Transcriptional regulation of the sucrase gene of Staphylococcus xylosus by the repressor ScrR. J. Bacteriol. 178:462-469.
6. Hiratsuka, Y., B. Wang, Y. Sato, and H. Kuramitsu. 1998. Regulation of sucrose-6-phosphate hydrolase activity in Streptococcus mutans: characterization of the scrR gene. Infect. Immun. 66:3736-3743.
7. Ishihara, K., T. Miura, H. K. Kuramitsu, and K. Okuda. 1996. Characterization of the Treponema denticola prtP gene encoding a prolyl-phenylalanine-specific protease (dentilisin). Infect. Immun. 64:5178-5186.
8. LeBlanc, D. J., L. N. Lee, and J. M. Inamine. 1991. Cloning and nucleotide base sequence analysis of a spectinomycin adenyltransferase AAD(9) determinant from Enterococcus faecalis. Antimicrob. Agents Chemother. 35:1804-1810.
9. Lueslnk, E. J., J. D. Marugg, O. P. Kuipers, and W. M. de Vos. 1999. Characterization of the divergent sacBK and sacAR operons, involved in sucrose utilization by Lactococcus lactis. J. Bacteriol. 181:1924-1926.
10. Munro, C., S. M. Michalek, and F. L. Macrina. 1991. Cariogenicity of Streptococcus mutans V403 glucosyltransferase and fructosyltransferase mutants constructed by allelic exchange. Infect. Immun. 59:2316-2323.
11. Newbrun, E. 1983. Cariology, 2nd ed. Williams and Wilkins, Baltimore, Md.
12. Perry, D., L. M. Wondrack, and H. K. Kuramitsu. 1983. Genetic transformation of putative cariogenic properties in Streptococcus mutans. Infect. Immun. 41:722-727.
13. Postma, P. W., J. W. Lengeler, and G. R. Jacobson. 1993. Phosphoenolpyruvate: carbohydrate phosphotransferase systems of bacteria. Microbiol. Rev. 57:543-594.
14. Poy, F., and G. R. Jacobson. 1990. Evidence that a low-affinity sucrose phosphotransferase activity in Streptococcus mutans GS-5 is a high-affinity trehalose uptake system. Infect. Immun. 58:1479-1480.
15. Reid, S. J. M. S. Rafudeen, and N. G. Leat. 1999. The genes controlling sucrose utilization in Clostridium beijerinckii NCIMB 8052 constitute an operon. Microbiology 145:1461-1472.
16. Sato, Y., and H. K. Kuramitsu. 1988. Sequence analysis of the Streptococcus mutans scrB gene. Infect. Immun. 56:1956-1960.
17. Scr of the Streptococcus mutans phosphoenolpyruvate-dependent sucrose phosphotransferase system. J. Bacteriol. 171:263-271.
18. Sato, Y., Y. Yamamoto, H. Kizaki, and H. K. Kuramitsu. 1993. Isolation, characterization and sequence analysis of the scrK gene encoding fructokinase of Streptococcus mutans. J. Gen. Microbiol. 139:921-927.
19. Sato, Y., Y. Yamamoto, R. Suzuki, H. Kizaki, and H. K. Kuramitsu. 1991. Construction of scrA::lacZ gene fusions to investigate regulation of the sucrose PTS of Streptococcus mutans. FEMS Microbiol. Lett. 79:339-346.
20. Steinmetz, M. 1993. Carbohydrate cataholism: pathways, enzymes, genetic regulation and evolution. American Society for Microbiology, Washington, D.C.
21. Tanzer, J. M., B. M. Chassy, and M. I. Krichevsky. 1971. Sucrose metabolism by Streptococcus mutans, SL-1. Biochim. Biophys. Acta 261:379-387.
22. Tao, L., I. C. Sutcliffe, R. R. Russell, and J. J. Ferretti. 1993. Transport of sugars, including sucrose, by the msm transport system of Streptococcus mutans. J. Dent. Res. 72:1386-1390.
23. Weickert, M. J., and S. Adhya. 1992. A family of bacterial regulators homologous to Gal and Lac repressors. J. Biol. Chem. 267:15869-15874.
24. Yamashita, Y., T. Takehara, and H. K. Kuramitsu. 1993. Molecular characterization of a Streptococcus mutans mutant altered in environmental stress responses. J. Bacteriol. 175:6220-6228.