Estimation of the sulfated glycosaminoglycan content of bovine skin with Alcian Blue

Journal of the American Leather Chemists Association

Volumn 98, Issue 9, 2003, Pages 337-343

  Mozersky, Samuel M ^a   Wildermuth, Renee J ^a   Marmer, William N ^a  

^a EASTERN REGIONAL RESEARCH CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AGENTS; CENTRIFUGATION; CHEMICAL MODIFICATION; COMMINUTION; COMPOSITION EFFECTS; ESTIMATION; POLYSACCHARIDES; SKIN;

ALCIAN BLUE; BOVINE SKIN; COLLAGENASE; INCUBATION TIMES; REAGENT SOLUTIONS; RELATIVE STANDARD DEVIATION; SULFATED GLYCOSAMINOGLYCAN;

SULFUR COMPOUNDS;

EID: 0141480809     PISSN: 00029726     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (19)

1. Bjornsson, S.; Simultaneous preparation and quantitation of proteoglycans by precipitation with alcian blue. Anal. Biochem. 210, 282-291, 1993.
2. Karlsson, M., and Bjornsson, S.; Quantitation of proteoglycans in biological fluids using alcian blue. in Methods in Molecular Biology 171: Proteoglycan Protocols, (Iozzo, R. V. ed.) Humana Press, Totowa, New Jersey, pp. 159-173, 2001.
3. Alexander, K. T. W., Haines, B. M., and Walker, M. P.; Influence of proteoglycan removal on opening-up in the beamhouse. JALCA 81, 85-102, 1986.
4. Kronick, P. L., and Iandola, S. K.; Persistence of minority macromolecules of hide through the beamhouse. III. Persistence of decorin. JALCA 93, 148-155, 1998.
5. Bjornsson, S. V.; Personal communication to Mozersky, S.M., November 5, 2001.
6. Scott, J. E.; Alcian dyes: I.C.I. Alcian dyes: I.C.I. cease manufacture and release details of composition. Histochemie 37, 379-380, 1973.
7. Scott, J. E.; Histochemistry of alcian blue. II. The structure of alcian blue 8GX. Histochemie 30, 215-234, 1972.
8. Budavari, S., O'Neil, M. J., Smith, A., Heckelman, P. E., and Kinneary, J. F. (eds.); The Merck Index, 12th edition, page 41, item 1996.
9. Steedman, H. F.; Alcian Blue 8GS: A new stain for mucin. Quarterly Journal of Microscopical Science 91, 477-479, 1950.
10. Pearse, A. G. E.; Histochemistry, 2nd edition, Little, Brown and Company, Boston, Ma, pp. 70-71, 259-261, and 838, 1961.
11. Yamada, K.; Staining of sulfated polysaccharides by means of alcian blue. Nature 198, (4882), 799, 1963.
12. Scott, J. E.; How rational histochemistry produced order out of chaos in the "amorphous ground substance" (with a little help from biochemistry, biophysics etc.). Eur. J. Histochem. 42, Special Issue, 29-34, 1998.
13. Scott, J. E.; The solubility of cetylpyridinium complexes of biological polyanions in solutions of salts. Biochim. Biophys. Acta 18, 428-429, 1955.
14. Scott, J. E.; Aliphatic ammonium salts in the assay of acidic polysaccharides from tissues. in Methods of Biochemical Analysis, 8, (Glick, D. ed.) Interscience Publishers, Inc., New York, pp. 145-197, 1960.
15. Scott, J. E., and Dorling, J.; Differential staining of glycosaminoglycans (mucopolysaccharides) by Alcian Blue in salt solutions. Histochemie 5, 221-233, 1965.
16. Mowry, R. W.; Alcian Blue technics for the histochemical study of acidic carbohydrates. J. Histochem. Cytochem. 4, 407, 1956.
17. Newton, D. J., Scott, J. E., and Whiteman, P.; The estimation of acid glycosaminoglycan-Alcian Blue complexes eluted from electrophoretic strips. Anal. Biochem. 62, 268-273, 1974.
18. Hascall, V. C., and Kimura, J. H.; Proteoglycans: isolation and characterization. in Methods in Enzymology, 82, (Colowick, S.P., and Kaplan, N.O. eds) Academic Press, New York, pp. 769-800, 1982.
19. Deming, W. E.; Statistical adjustment of Data, Dover Publications, New York, 1964, p. 43.