Expressing PHB synthetic genes through chloroplast genetic engineering

Chinese Science Bulletin

Volumn 47, Issue 16, 2002, Pages 1373-1376

  Zhang, Jingyu ^a   Su, Ning ^b   Zhang, Zhonglin ^b   Zhao, Huayan ^a   Zhu, Shengwei ^a   Song, Yanru ^a  

^a INSTITUTE OF BOTANY   (China)

^b INSTITUTE OF PLANT PROTECTION   (China)

Author keywords

Chloroplast genetic engineering; Gene silencing; Poly 3 hydroxybutyrate; Transcriptional level; Transplastomic tobacco

Indexed keywords

POLY(3 HYDROXYBUTYRIC ACID);

ARTICLE; CHLOROPLAST; CONTROLLED STUDY; GENE EXPRESSION; GENETIC ENGINEERING; GENOME; NONHUMAN; POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SOUTHERN BLOTTING; TOBACCO;

NICOTIANA TABACUM;

EID: 0043271573     PISSN: 10016538     EISSN: None     Source Type: Journal    
DOI: 10.1360/02tb9303     Document Type: Article

References (12)

1. Van der Walle, G. A., De Koning, G. J., Weusthuis, R. A. et al., Properties, modifications and applications of biopolyesters, Adv. Biochem. Eng. Biotechnol., 2001, 71: 263.
2. Hanley, Z., Slabas, T., Elborough, K. M., The use of plant biotechnology for the production of biodegradable plastics, Trends Plant Sci., 2000, 5(2): 45.
3. Ye, L., Li, C., Song, Y. R., Construction of plant seed-specific expression vectors pSCB and pSCAB and the obtainment of transgenic Brassica napus H165 expression poly-3-bydroxybutyrate synthetic genes, Chinese Science Bulletin, 2000, 45(5): 516.
4. Heifetz, P. B., Genetic engineering of the chloroplast, Biosci. Biotechnol. Biochem., 2001, 65(7): 1688.
5. Zhang, Z. L., Zhang, J. Y., Li, Y. N. et al.. Cloning of homologous targeting sequences containing chloroplast genes, rbcL and atpB, of Brassica napus and construction of plastid transformation vectors harboring key enzyme genes responsible for PHB biosynthesis, Acta Phytophysiologica Sinica, 2001, 27(3): 235.
6. Svab, Z., Maliga, P., High-frequency plastid transformation in tobacco by selection for a chimeric aadA gene, Proc. Natl. Acad. Sci. USA, 1993, 90: 913.
7. Edwards, K., Johnstone, C., Thompson, C., A simple and rapid method for the preparation of plant genomic DNA for PCR analysis, Nucl. Acids. Res., 1991, 19: 1349.
8. Gong, X. S., Zeng, F. H., Yan, L. F., An efficient method for the purification of chloroplast DNA from higher plants, J. Wuhan Botanical Res., 1994, 12(3): 277.
9. Sambrook, J., Fritsch, E. F., Maniatis, T., Molecular Cloning: A Laboratory Manual, 2nd ed., New York: Cold Spring Harbor Laboratory Press, 1989, 362; 463.
10. Luo, P., Wang, G. D., Chen, X. Y., Isolation and expression analysis of two cDNAs encoding C4H homologues from Gossypium arboreum., Acta Bot. Sin., 2001, 43(1): 77.
11. Zhang, J. Y., Ye, L., Li, L. et al., Obtainment of transgenic tobacco harboring phbA, phbB and phbC genes by twice transformation, Acta Bot. Sin., 2001, 43(1): 59.
12. Van Bel, A. J. E., Hibberd, J., Prüfer, D. et al., Novel approach in plastid transformation, Current Opinion in Biotechnology, 2001, 12: 144.