The establishment and characterization of an immortal cell line with a stable chondrocytic phenotype

Journal of Cellular Biochemistry

Volumn 89, Issue 5, 2003, Pages 992-1004

  King, Karen B ^a,b   Kimura, James H ^a  

^a HENRY FORD HEALTH SYSTEM   (United States)

^b Department of SSHA   (United States)

Author keywords

Chondrocytes; Chondrosarcoma; Extracellular matrix; Metabolism; Proteoglycan

Indexed keywords

PROTEOGLYCAN;

ANIMAL CELL; ANIMAL MODEL; ARTICLE; CARTILAGE CELL; CELL CLONE; CELL IMMORTALIZATION; CELL LINE; CELL TYPE; CHONDROSARCOMA; ELECTRON MICROSCOPY; EXTRACELLULAR MATRIX; METABOLISM; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; RAT; TISSUE CULTURE; TUMOR CELL;

ANIMALS; CELL COUNT; CELL LINE; CHONDROCYTES; CHONDROITIN SULFATES; CHONDROSARCOMA; CLONE CELLS; CULTURE MEDIA; DISACCHARIDES; DNA; EXTRACELLULAR MATRIX; GLYCOSAMINOGLYCANS; HYALURONIC ACID; INSULIN; MICROSCOPY, ELECTRON; PHENOTYPE; PROTEOGLYCANS; RATS; SULFUR RADIOISOTOPES;

EID: 0043270545     PISSN: 07302312     EISSN: None     Source Type: Journal    
DOI: 10.1002/jcb.10571     Document Type: Article

References (50)

1. Arai M, Yada T, Suzuki S, Kimata K. 1992. Isolation and characterization of type IX collagen-proteoglycan from the Swarm rat chondrosarcoma. Biochemica Biophysica Acta 1117:60-70.
2. Bosserhoff AK, Kondo S, Moser M, Dietz UH, Copeland NG, Gilbert DJ, Jenkins NA, Buettner R, Sandell LJ. 1997. Mouse CD-RAP/MIA gene: Structure, chromosomal localization, and expression in cartilage and chondrosarcoma. Dev Dyn 208:516-525.
3. Calabro A, Hascall VC. 1994. Differential effects of brefeldin A on chondroitin sulfate and hyaluronan synthesis in rat chondrosarcoma cells. J Biol Chem 269:22764-22770.
4. Calabro A, Hascall VC, Caterson B. 1992. Monoclonal antibodies directed against epitopes within the core protein structure of the large aggregating proteoglycan (aggrecan) from the swarm rat chondrosarcoma. Arch Biochem Biophys 298:349-360.
5. Caterson B, Baker JR, Christner JE, Lee Y, Lentz M. 1985. Monoclonal antibodies as probes for determining the microheterogeneity of the link proteins of cartilage proteoglycan. J Biol Chem 260:11348-11356.
6. Choi HU, Meyer K, Swarm R. 1971. Mucopolysaccharide and proteinpolysaccharide of a transplantable rat chondrosarcoma. Proc Natl Acad Sci USA 68:877-879.
7. Di Cesare PE, Carlson CS, Attur M, Kale AA, Abramson SB, Della Valle C, Steiner G, Amin AR. 1998. Upregulation of inducible nitric oxide synthase and production of nitric oxide by the Swarm rat and human chondrosarcoma. J Orthop Res 16:667-674.
8. Doege K, Hassell JR, Caterson B, Yamada Y. 1986. Link protein cDNA sequence reveals a tandemly repeated protein structure. Proc Natl Acad Sci USA 83:3761-3765.
9. Doege K, Sasaki M, Horigan E, Hassel JR,YamadaY. 1987. Complete primary structure of the rat cartilage proteoglycan core protein deduced from cDNA clones. J Biol Chem 262:17757-17767.
10. Faltz LL, Caputo CB, Kimura JH, Schrode J, Hascall VC. 1979. Structure of the complex between hyaluronic acid, the hyaluronic acid-binding region, and the link protein of proteoglycan aggregates from the swarm rat chondrosarcoma. J Biol Chem 254:1381-1387.
11. Fang J, Shing Y, Wiederschain D, Yan L, Butterfield C, Jackson G, Harper J, Tamvakopoulos G, Moses MA. 2000. Matrix metalloproteinase-2 is required for the switch to the angiogenic phenotype in tumor model. Proc Natl Acad Sci USA 97:3884-3889.
12. Fang J, Yan L, Shing Y, Moses MA. 2001. HIF-1alpha-mediated up-regulation of vascular endothelial growth factor, independent of basic fibroblast growth factor, is important in the swtich to the angiogenic phenotype during early tumorigenesis. Cancer Res 61:5731-5735.
13. Farndale RW, Sayers CA, Barrett AJ. 1982. A direct spectrophotometric microassay for sulfated glycosaminoglycans in cartilage cultures. Connect Tissue Res 9:247-248.
14. Fellini SA, Hascall VC, Kimura JH. 1984. Localization of proteoglycan core protein in subcellular fractions isolated from rat chondrosarcoma chondrocytes. J Biol Chem 259:4634-4641.
15. Fernandes RJ, Schmid TM, Harkey MA, Eyre DR. 1997. Incomplete processing of type II procollagen by a rat chondrosarcoma cell line. Eur J Biochem 247:620-624.
16. Fernandes RJ, Hirohata S, Engle JM, Colige A, Cohn DH, Eyre DR, Apte SS. 2001. Procollagen II amino propeptide processing by ADAMTS-3. Insights on dermatosparaxis. J Biol Chem 276:31502-31509.
17. Foley TPJ, Nissley SP, Stevens RL, King GL, Hascall VC, Humbel RE, Short PA, Rechler MM. 1982. Demonstration of receptors for insulin and insulin-like growth factors on Swarm rat chondrosarcoma chondrocytes, Evidence that insulin stimulates proteoglycan synthesis through the insulin receptor. J Biol Chem 257:663-669.
18. Hascall GK. 1980. Ultrastructure of the chondrocytes and extracellular matrix of the swarm rat chondrosarcoma. Anat Rec 198:135-146.
19. Hascall GK, Kimura JH. 1981. The ultrastructure of cultures from the Swarm rat chondrosarcoma. Anat Rec 200:287-292.
20. Hering TM, Sandell LJ. 1988. Biosynthesis and cell-free translation of Swarm rat chondrosarcoma and bovine cartilage link proteins. J Biol Chem 263:1030-1036.
21. Hunziker EB, Graber W. 1986. Differential extraction of proteoglycans from cartilage tissue matrix compartments in isotonic buffer salt solutions and commercial tissue-culture media. J Histochem Cytochem 34:1149-1153.
22. Hunziker EB, Herrmann W, Schenk RK. 1983. Ruthenium hexammine trichloride (RHT)-mediated interaction between plasmalemmal components and pericellular matrix proteoglycans is responsible for the preservation of chondrocytic plasma membranes in situ during cartilage fixation. J Histochem Cytochem 31:717-727.
23. Kim Y-J, Sah RL, Doong JY, Grodzinsky AJ. 1988. Fluorometric assay of DNA in cartilage explants using Hoechst 33258. Anal Biochem 174:168-176.
24. Kimata K, Foidart JM, Pennypacker JP, Kleinman HK, Martin GR, Hewitt AT. 1982. Immunofluorescence localization of fibronectin in chondrosarcoma cartilage matrix. Cancer Res 42:2384-2391.
25. Kimura JH, Hardingham TE, Hascall VC, Solursh M. 1979. Biosynthesis of proteoglycans and their assembly into aggregates in cultures of chondrocytes from the Swarm rat chondrosarcoma. J Biol Chem 254:2600-2609.
26. Kimura JH, Hardingham TE, Hascall VC. 1980. Assembly of newly synthesized proteoglycan and link protein into aggregates in cultures of chondrosarcoma chondrocytes. J Biol Chem 255:7134-7143.
27. Kimura JH, Thonar EJ-M, Hascall VC, Reiner A, Poole AR. 1981. Identification of core protein, an intermediate in proteoglycan biosynthesis in cultured chondrocytes from the Swarm rat chondrosarcoma. J Biol Chem 256:7890-7897.
28. Kimura JH, Lohmander LS, Hascall VC. 1984. Studies on the biosynthesis of cartilage proteoglycan in a model system of cultured chondrocytes from the Swarm rat chondrosarcoma. J Cell Biochem 26:261-278.
29. Kimura JH, Helenius J, King KB, Gibson GJ, Schaffier MB, Plaas AHK. 1995. Biosynthesis of aggrecan in a mutant cell line with a reduced intracellular sulfate precursor pool: "41st Annual Meeting of the Orthopaedic Research Society." Orlando. FL, p 427.
30. King KB, Chubinskaya S, Reid DL, Madsen LH, Mollenhauer J. 1997. Absence of cell-surface annexin V is accompanied by defective collagen matrix binding in the Swarm rat chondrosarcoma. J Cell Biochem 65:131-144.
31. Kittelberger-Ewert R, Hinz N, Oechsner I, Schroeter-Kermani C, Barrach HJ. 1988. Production and specificity of antibodies against the central region of type II collagen. Immunol Invest 17:49-61.
32. Kucharska AM, Kuettner KE, Kimura JH. 1990. Biochemical characterization of long-term culture of the Swarm rat chondrosarcoma chondrocytes in agarose. J Orthop Res 8:781-792.
33. Larramendi PC. 1988. Method for obtaining a cross section layer of tissue culture for EM without pelleting or reembedding. J Electron Microsc Tech 10:119-121.
34. Lohmander LS, Fellini S, Kimura JH, Stevens JW, Hascall VC. 1983. Formation of proteoglycan aggregated in rat chondrosarcoma chondrocyte cultures treated with tunicamycin. J Biol Chem 258:12280-12286.
35. Mason RM, Kimura JH, Hascall VC. 1982. Biosynthesis of hyaluronic acid in cultures of chondrocytes from the Swarm rat chondrosarcoma. J Biol Chem 257:2236-2245.
36. Matsumura T, Whelan MC, Li XQ, Trippel SB. 2000. Regulation by IGF-I and TGF-betal of Swarm-rat chondrosarcoma chondrocytes. J Orthop Res 18:351-355.
37. Morcuende JA, Plaas AHK, Kimura JH. 1996. Altered functional activity of the sulfate transport channel SAT-1 in a mutant rat chondrosarcoma clone: "42nd Annual Meeting, Orthopaedic Research Society." Atlanta, GA, p 764.
38. Moses MA, Shing Y. 1994. Production of matrix metalloproteinases and a metalloproteinase inhibitor by Swarm rat chondrosarcoma. Biochem Biophys Res Commun 199:418-424.
39. Mukhopadhyay K, Lefebvre V, Zhou G, Garofalo S, Kimura JH, de Crombrugghe B. 1995. Use of a new rat chondrosarcoma cell line to delineate a 119-base pair chondrocyte-specific enhancer element and to define active promoter segments in the mouse pro-alphal(II) collagen gene. J Biol Chem 270:27711-27719.
40. Mörgelin M, Heinegård D, Engel J, Paulsson M. 1992. Electron microscopy of native cartilage oligomeric matrix protein purified from the Swarm rat chondrosarcoma reveals a five-armed structure. J Biol Chem 267:6137-6141.
41. Oegema TR, Jr., Brown M, Dziewiatkowski DD. 1977. The link protein in proteoglycan aggregates from the Swarm rat chondrosarcoma. J Biol Chem 252:6470-6477.
42. Olin AI, Mörgelin M, Sasaki T, Timpl R, Heinegård D, Aspberg A. 2001. The proteoglycans aggrecan and versican form networks with fibulin-2 through their lectin domain binding. J Biol Chem 276:1253-1261.
43. Rhodes C, Savagner P, Line S, Sasaki M, Chirigos M, Doege K, Yamada Y. 1991. Characterization of the promoter for the rat and human link protein gene. Nucleic Acids Res 19:1933-1939.
44. Saito S, Zeck B, Inerot S, Kuettner KE, Kimura JH. 1988. A long term suspension cultures of cells from the Swarm rat chondrosarcoma after alginate preculture. Keio J Med 37:282-298.
45. Seong S-C, Matsumura T, Lee FY, Whelan MC, Li XQ, Trippel SB. 1994. Insulin-like growth factor I regulation of Swarm rat chondrosarcoma chondrocytes in culture. Exp Cell Res 211:238-244.
46. Stevens RL, Nissley SP, Kimura JH, Rechler MM, Caplan AI, Hascall VC. 1981. Effects of insulin and nultiplication-stimulating activity on proteoglycan biosynthesis in chondrocytes from the Swarm rat chondrosarcoma. J Biol Chem 256:2045-2052.
47. Sun D, Aydelotte MB, Maldonado B, Kuettner KE, Kimura JH. 1986. Clonal analysis of the population of chondrocytes from the Swarm rat chondrosarcoma in agarose culture. J Orthop Res 4:427-436.
48. Thonar EJ-MA, Lohmander LS, Kimura JH, Fellini SA, Yanagishita M, Hascall VC. 1983. Biosynthesis of O-linked oligosaccharides on proteoglycans by chodrocytes from the swarm rat chondrosarcoma. J Biol Chem 258:11564-11570.
49. Yamauchi S, Hirahara Y, Usui H, Takeda Y, Hoshino M, Fukuta M, Kimura JH, Habuchi O. 1999. Purification and characterization of chondroitin 4-sulfotransferase from the culture medium of a rat chondrosarcoma cell line. J Biol Chem 274:2456-2463.
50. Zhang J, Plaas AHK, Kimura JH. 1999. A clonal cell line derived from the Swarm rat chondrosarcoma is defective in expression of the sulfate transporter gene for diastrophic dysplasia: "45th Annual Meeting of the Orthopaedic Research Society." Anaheim, CA, p 132.