Analysis of nuclear DNA content in Capsicum (Solanaceae) by flow cytometry and feulgen densitometry

Annals of Botany

Volumn 92, Issue 1, 2003, Pages 21-29

  Moscone, Eduardo A ^a   Baranyi, Monika ^b   Ebert, Irma ^b   Greilhuber, Johann ^b   Ehrendorfer, Friedrich ^b   Hunziker, Armando T ^a  

^a Instituto Multidisciplinario de Biologia Vegetal   (Argentina)

^b UNIVERSITY OF VIENNA   (Austria)

Author keywords

Capsicum; Feulgen densitometry; Flow cytometry; Genome size variation; Karyosystematics; Karyotype evolution; Solanaceae

Indexed keywords

DNA; FLOW CYTOMETRY;

ALLIUM CEPA; CAMPYLOPODIUM; CAPSICUM; CAPSICUM ANNUUM; CAPSICUM ANNUUM VAR. ANNUUM; CAPSICUM BACCATUM; CAPSICUM BACCATUM VAR. BACCATUM; CAPSICUM BACCATUM VAR. PENDULUM; CAPSICUM CHACOENSE; CAPSICUM CHINENSE; CAPSICUM EXIMIUM; CAPSICUM FRUTESCENS; CAPSICUM PUBESCENS; DITTA; HORDEUM VULGARE; HORDEUM VULGARE SUBSP. VULGARE; MAGNOLIOPHYTA; SOLANACEAE;

COLORING AGENT; FUCHSINE; PLANT DNA;

ARTICLE; CELL NUCLEUS; CHEMISTRY; DENSITOMETRY; FEULGEN STAINING; FLOW CYTOMETRY; GENETICS; GENOME; HETEROCHROMATIN; KARYOTYPING; METHODOLOGY; PEPPER; PLANT CHROMOSOME; SPECIES DIFFERENCE;

CAPSICUM; CELL NUCLEUS; CHROMOSOMES, PLANT; COLORING AGENTS; DENSITOMETRY; DNA, PLANT; FLOW CYTOMETRY; GENOME, PLANT; HETEROCHROMATIN; KARYOTYPING; ROSANILINE DYES; SPECIES SPECIFICITY;

EID: 0043236225     PISSN: 03057364     EISSN: None     Source Type: Journal    
DOI: 10.1093/aob/mcg105     Document Type: Article

References (54)

1. Arumuganathan K, Earle ED. 1991. Nuclear DNA content of some important plant species. Plant Molecular Biology Reporter 9: 208-218.
2. Baranyi M, Greilhuber J. 1995. Flow cytometric analysis of genome size variation in cultivated and wild Pisum sativum (Fabaceae). Plant Systematic and Evolution 194: 231-239.
3. Baranyi M, Greilhuber J. 1996. Flow cytometric and Feulgen densitometric analysis of genome size variation in Pisum. Theoretical and Applied Genetics 92: 297-307.
4. Belletti P, Marzachi C, Lanteri S. 1998. Flow cytometric measurement of nuclear DNA content in Capsicum (Solanaceae). Plant Systematics and Evolution 209: 85-91.
5. Bennett MD, Leitch IJ. 1995. Nuclear DNA amounts in angiosperms. Annals of Botany 76: 113-176.
6. Bennett MD, Smith JB. 1976. Nuclear DNA amounts in angiosperms. Philosophical Transactions of the Royal Society of London B 274: 227-274.
7. Bennett MD, Cox AV, Leitch IJ. 2000a. Angiosperm DNA C-values database. http://www.rbgkew.org.uk/cval/database1.html
8. Bennett MD, Bhandol P, Leitch IJ. 2000b. Nuclear DNA amounts in angiosperms and their modern uses - 807 new estimates. Annals of Botany 86: 859-909.
9. Bennett MD, Johnston S, Hodnett GL, Price HJ. 2000c. Allium cepa L. cultivars from four continents compared by flow cytometry show nuclear DNA constancy. Annals of Botany 85: 351-357.
10. Boiteux LS, Nagata T, Dutra WP, Fonseca MEN. 1993. Sources of resistance to tomato spotted wilt virus (TSWV) in cultivated and wild species of Capsicum. Euphytica 67: 89-94.
11. Boukema IW. 1980. Allelism of genes controlling resistance to TMV in Capsicum L. Euphytica 29: 433-439.
12. Boukema IW. 1983. Research on the location of the gene for resistance to TMV in Capsicum chacoense Hunz. and male sterility in progenies from the cross C. chacoense x C. annuum L. Proceedings of the 5th Meeting of the Capsicum and Eggplant working group of Eucarpia, Clovdiv, Bulgaria, 1983, 84-87.
13. Brandham PE, Doherty M-J. 1998. Genome size variation in the Aloaceae, an angiosperm family displaying karyotypic orthoselection. Annals of Botany 82 (Suppl. A): 67-73.
14. De Laat AMM, Göhde W, Vogelzang MJDC. 1987. Determination of ploidy of single plants and plant populations by flow cytometry. Plant Breeding 99: 303-307.
15. Doležel J. 1991. Flow cytometric analysis of nuclear DNA content in higher plants. Phytochemical Analysis 2: 143-154.
16. Doležel J, Bartoš J, Voglmayr H, Greilhuber J. 2003. Nuclear DNA content and genome size of trout and human. Cytometry 51 A: 127-128.
17. Ellis RH, Hong TD, Roberts EH. 1985. Handbook of seed technology for genebanks. 2. Compendium of specific germination, information, and test recommendations. Handbook for genebanks 3. Rome: International Board for Plant Genetic Resources.
18. Eshbaugh WH. 1979. Biosystematic and evolutionary study of the Capsicum pubescens complex. National Geographic Society Research Reports (USA) 1970: 143-162.
19. Eshbaugh WH. 1993. Peppers: history and exploitation of a serendipitous new crop discovery. In: Janick J, Simon JE, eds. New crops. New York: J Wiley and Sons, 132-139.
20. Flavell RB. 1986. Repetitive DNA and chromosome evolution in plants. Philosophical Transactions of the Royal Society of London B 312: 227-242.
21. Galbraith DW, Harkins KR, Maddox JM, Ayres NM, Sharma DP, Firoozabady E. 1983. Rapid flow cytometric analysis of the cell cycle in intact plant tissues. Science 220: 1049-1051.
22. Greilhuber J. 1988. 'Self-tanning' - a new and important source of stoichiometric error in cytophotometric determination of nuclear DNA content in plants. Plant Systematics and Evolution 158: 87-96.
23. Greilhuber J. 1995. Chromosomes of the monocotyledons (general aspects). In: Rudall PJ, Cribb PJ, Cutler DF, Humphries CJ, eds. Monocotyledons: systematics and evolution. Kew: Royal Botanic Gardens, 379-414.
24. Greilhuber J. 1998. Intraspecific variation in genome size: a critical reassessment. Annals of Botany 82 (Suppl. A): 27-35.
25. Greilhuber J, Ebert I. 1994. Genome size variation in Pisum sativum. Genome 37: 646-655.
26. Heiser CB. 1995. Peppers: Capsicum (Solanaceae). In: Smartt J, Simmonds NW, eds. Evolution of crop plants. Essex: Longman, 449-451.
27. Heiser CB, Smith PG. 1953. The cultivated Capsicum peppers. Economic Botany 7: 214-227.
28. Hunziker AT. 1950. Estudios sobre Solanaceae. I. Sinopsis de las especies silvestres de Capsicum de Argentina y Paraguay. Darwiniana 9: 225-247.
29. Hunziker AT. 1956. Synopsis of the genus Capsicum. VIII Congrès International de Botanique, Paris, 1954. Compte Rendu des Séances Rapports et Communications 4: 73-74.
30. Hunziker AT. 1979. South American Solanaceae: a synoptic survery. In: Hawkes JG, Lester RN, Skelding AD, eds. The biology and taxonomy of the Solanaceae. Linnean Society Symposium 7: 49-85. London: Academic Press.
31. Hunziker AT. 1998. Estudios sobre Solanaceae. XLVI. Los ajíes silvestres de Argentina (Capsicum). Darwiniana 36: 201-203.
32. Hunziker AT. 2001. Genera Solanacearum: the genera of Solanaceae illustrated, arranged according to a new system. Liechtenstein: ARG Gantner Verlag K.-G.
33. Hunziker AT, Barboza GE. 1998. Estudios sobre Solanaceae XLV. Sobre la presencia de Exodeconus en Argentina y una novedad en Capsicum baccatum. Kurtziana 26: 23-31.
34. Lippert LF, Smith PG, Bergh BO. 1966. Cytogenetics of the vegetable crops. Garden pepper, Capsicum sp. Botanical Review 32: 24-55.
35. McLeod MJ, Guttman SI, Eshbaugh WH, Rayle RE. 1983. An electrophoretic study of evolution in Capsicum (Solanaceae). Evolution 37: 562-574.
36. Moscone EA. 1989. Estudios citotaxonómicos en las tribus Solaneae y Nicotianeae (Solanaceae) de América del Sur. PhD Thesis, University of Córdoba, Argentina.
37. Moscone EA. 1999. Análisis cariotípico en Capsicum baccatum var. umbilicatum (Solanaceae) mediante bandeos AgNOR y de fluorescencia. Kurtziana 27: 225-232.
38. Moscone EA, Lambrou M, Ehrendorfer F. 1996. Fluorescent chromosome banding in the cultivated species of Capsicum (Solanaceae). Plant Systematics and Evolution 202: 37-63.
39. Moscone EA, Lambrou M, Hunziker AT, Ehrendorfer F. 1993a. Giemsa C-banded karyotypes in Capsicum (Solanaceae). Plant Systematics and Evolution 186: 213-229.
40. Moscone EA, Lambrou M, Ehrendorfer F, Hunziker AT. 1993b. Fluorescent chromosome banding in Capsicum (Solanaceae). In: Heubl GR, ed. Jornadas de Taxonomía Vegetal, Munich, Germany, September 13-15, 1993, Lecture Abstracts, 8.
41. Moscone EA, Loidl J, Ehrendorfer F, Hunziker AT. 1995. Analysis of active nucleolus organizing regions in Capsicum (Solanaceae) by silver staining. American Journal of Botany 82: 276-287.
42. Mukherjee S, Sharma AK. 1990. Intraspecific variation of nuclear DNA in Capsicum annuum L. Proceedings of the Indian Academy of Science (Plant Science) 100: 1-6.
43. Narayan RKJ. 1987. Nuclear DNA changes, genome differentiation and evolution in Nicotiana (Solanaceae). Plant Systematics and Evolution 157: 161-180.
44. Pickersgill B. 1988. The genus Capsicum: a multidisciplinary approach to the taxonomy of cultivated and wild plants. Biologisches Zentralblatt 107: 381-389.
45. Pickersgill B. 1991. Cytogenetics and evolution of Capsicum L. In: Tsuchiya T, Gupta PK, eds. Chromosome engineering in plants: genetics, breeding, evolution, part B. Amsterdam: Elsevier, 139-160.
46. Pickersgill B, Heiser CB, McNeill J. 1979. Numerical taxonomic studies on variation and domestication in some species of Capsicum. In: Hawkes JG, Lester RN, Skelding AD, eds. The biology and taxonomy of the Solanaceae. Linnean Society Symposium 7. London: Academic Press, 678-700.
47. Pringle GJ, Murray BG. 1991. Karyotype diversity and nuclear DNA variation in Cyphomandra. In: Hawkes JG, Lester RN, Nee M, Estrada N, eds. Solanaceae III: taxonomy, chemistry, evolution. London: The Royal Botanic Gardens Kew for The Linnean Society of London, 247-252.
48. Pringle GJ, Murray BG. 1993. Karyotypes and C-banding patterns in species of Cyphomandra Mart. ex Sendtner (Solanaceae). Botanical Journal of the Linnean Society 111: 331-342.
49. Rohlf FJ. 1992. BIOM. A package of statistical programs to accompany the text 'Biometry'. New York: Applied Biostatistics.
50. Schweizer D. 1976. Reverse fluorescent chromosome banding with chromomycin and DAPI. Chromosoma 58: 307-324.
51. Sokal RR, Rohlf FJ. 1995. Biometry, the principles and practice of statistics in biological research. New York: Freeman.
52. Stiefkens L, Bernardello G. 2000. Karyotypes and DNA content in diploid and polyploid Lycium (Solanaceae). Boletín de la Sociedad Argentina de Botánica 35: 237-244.
53. Walsh BM, Hoot SB. 2001. Phylogenetic relationships of Capsicum (Solanaceae) using DNA sequences from two noncoding regions: the chloroplast atpB-rbcL spacer region and nuclear waxy introns. International Journal of Plant Sciences 162: 1409-1418.
54. White J, Rees H. 1985. The chromosome cytology of a somatic hybrid petunia. Heredity 55: 53-59.