Improvement of the yield of physiologically active oligosaccharides in continuous hydrolysis of chitosan using immobilized chitosanases

Biotechnology and Bioengineering

Volumn 84, Issue 1, 2003, Pages 121-127

  Kuroiwa, Takashi ^a   Ichikawa, Sosaku ^a   Sato, Seigo ^a   Mukataka, Sukekuni ^a  

^a UNIVERSITY OF TSUKUBA   (Japan)

Author keywords

Chitosan oligosaccharide; Chitosanase; Damk hler number; Immobilized enzyme; Packed bed reactor

Indexed keywords

CHEMICAL REACTORS; ENZYMES; HYDROLYSIS; MASS TRANSFER; POLYSACCHARIDES;

COLUMN REACTORS;

OLIGOMERS;

CHITOSAN; CHITOSANASE; ENZYME; OLIGOSACCHARIDE;

ARTICLE; CHEMICAL REACTION KINETICS; DENSITY; ENZYME IMMOBILIZATION; FLOW RATE; HYDROLYSIS; METHODOLOGY; PACKED BED REACTOR; PROCESS DESIGN; PROTEIN PROCESSING;

BIOREACTORS; ENZYME ACTIVATION; ENZYME STABILITY; ENZYMES, IMMOBILIZED; GLYCOSIDE HYDROLASES; HYDROLYSIS; OLIGOSACCHARIDES; QUALITY CONTROL;

EID: 0043195258     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10758     Document Type: Article

References (22)

1. Bailey JE, Ollis DF. 1986. Biochemical engineering fundamentals. New York: McGraw-Hill. p 204-208.
2. Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of the protein-dye binding. Anal Biochem 72:248-254.
3. Fukamizo T, Ohkawa T, Ikeda Y, Goto S. 1994. Specificity of chitosanase from Bacillus pumilus. Biochim Biophys Acta 1205:183-188.
4. Guisan JM. 1988. Aldehyde-agarose gels as activated supports for immobilization-stabilization of enzymes. Enzyme Microb Technol 10:375-382.
5. Hirano S, Iwata M, Yamanaka K, Tanaka H, Toda T, Inui H. 1991. Enhancement of serum lysozyme activity by injecting a mixture of chitosan oligosaccharides intravenously in rabbits. Agric Biol Chem 55:2623-2625.
6. Ichikawa S, Takano K, Kuroiwa T, Hiruta O, Sato S, Mukataka S. 2002. Immobilization and stabilization of chitosanase by multipoint attachment to agar gel support. J Biosci Bioeng 93:201-206.
7. Imoto T, Yagishita K. 1971. A simple activity measurement of lysozyme. Agric Bio Chem 35:1154-1156.
8. Jeon YJ, Kim SK. 2000a. Continuous production of chitooligosaccharides using a dual reactor system. Proc Biochem 35:623-632.
9. Jeon YJ, Kim SK. 2000b. Production of chitooligosaccharides using an ultrafiltration membrane reactor and their antibacterial activity. Carbohydr Polym 41:133-141.
10. Jeon YJ, Park PJ, Kim SK. 2001. Antimicrobial effect of chitooligosaccharides produced by bioreactor. Carbohydr Polym 44:71-76.
11. Kendra DF, Hadweiger LA. 1984. Characterization of the smallest chitosan oligomer that is maximally antifungal to Fusarium solani and elicits pisatin formation in Pisum sativum. Exp Mycology 8:276-281.
12. Kimura S, Nakao S. 1985. Ultrafiltration. In: The Membrane Society of Japan, editor. Design methods for membrane separation processes. Tokyo: Kitami Syobo. p 37-49. (in Japanese)
13. Kuroiwa T, Ichikawa S, Hiruta O, Sato S, Mukataka S. 2002. Factors affecting the composition of oligosaccharides produced in chitosan hydrolysis using immobilized chitosanases. Biotechnol Prog 18:969-974.
14. Levenspiel O. 1999. Chemical reaction engineering (3rd ed.). New York: John Wiley & Sons. p 257-277.
15. Suzuki K, Mikami T, Okawa Y, Tokoro A, Suzuki S, Suzuki M. 1986. Antitumor effect of hexa-N-acetylchitohexaose and chitohexaose. Carbohydr Res 151:403-408.
16. Tichacek LJ. 1963. Selectivity in experimental reactors. AIChE J 9:394-399.
17. Tokoro A, Tatewaki N, Suzuki K, Mikami T, Suzuki S. 1988. Growth-inhibitory effect of hexa-N-acetylchitohexaose and chitohexaose against Meth-A solid tumor. Chem Pharm Bull 36:784-790.
18. Uchida Y, Izume M, Ohtakara A. 1989. Preparation of chitosan oligomers with purified chitosanase and its application. In: Skjak-Braek G, Anthonsen T, Sandford P, editors. Proceedings of the 4th International Conference on Chitin Chitosan. Oxford, UK: Elsevier Applied Science. p 373-382.
19. Uchida Y, Ohtakara A. 1988. Chitosanase from Bacillus species. Methods Enzymol 161B:501-505.
20. Wang W, Bo S, Li S, Qin W. 1991. Determination of the Mark-Houwink equation for chitosans with different degrees of deacetylation. Int J Biol Macromol 13:281-285.
21. Wilson EJ, Geankoplis CJ. 1966. Liquid mass transfer at very low Reynolds numbers in packed beds. Ind Eng Chem Fundamentals 5:9-14.
22. Yamane T. 1977. A proposal for accurate determination of "true" fractional retention of enzyme activity on immobilization. Biotechnol Bioeng 19:749-756.