Cloning and expression in Escherichia coli of the gene encoding Streptomyces PMF PLD, a phospholipase D with high transphosphatidylation activity

Enzyme and Microbial Technology

Volumn 33, Issue 5, 2003, Pages 676-688

  Zambonelli, Carlo ^a,d   Morandi, Paola ^b   Vanoni, Maria Antonietta ^b   Tedeschi, Gabriella ^b   Servi, Stefano ^a,c   Curti, Bruno ^b   Carrea, Giacomo ^a   Di Lorenzo, Rossana ^a   Monti, Daniela ^a  

^a CNR   (Italy)

^b UNIVERSITY OF MILAN   (Italy)

^c POLITECNICO DI MILANO   (Italy)

^d BOSTON COLLEGE   (United States)

Author keywords

Cloning; Heterologous expression; Phospholipase D; Streptomyces PMF

Indexed keywords

ANTIBIOTICS; BIOCATALYSTS; DNA; ENZYMES; ESCHERICHIA COLI;

GENE ENCODING;

GENETIC ENGINEERING;

ALCOHOL; DNA; PHOSPHATIDIC ACID; PHOSPHOLIPASE D; PHOSPHOLIPID; SIGNAL PEPTIDE;

ARTICLE; BACTERIAL CELL; BACTERIAL GENE; BIOCATALYST; CATALYSIS; CLONING; CORRELATION ANALYSIS; CRYSTALLOGRAPHY; CULTURE MEDIUM; DNA HYBRIDIZATION; DNA SEQUENCE; ENZYME ACTIVITY; ENZYME MECHANISM; ENZYME PURIFICATION; ENZYME STRUCTURE; ENZYME SYNTHESIS; ESCHERICHIA COLI; GENE AMPLIFICATION; GENE ISOLATION; GENE SEQUENCE; NONHUMAN; PHOSPHOLIPID SYNTHESIS; PLD GENE; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SEQUENCE ANALYSIS; STREPTOMYCES ANTIBIOTICUS; TRANSESTERIFICATION; WESTERN BLOTTING;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; STREPTOMYCES; STREPTOMYCES ANTIBIOTICUS;

EID: 0042882414     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(03)00190-X     Document Type: Article

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