Preparation of enzyme conjugate through adipic acid dihydrazide as linker and its use in immunoassays

Clinical Chemistry

Volumn 49, Issue 8, 2003, Pages 1410-1412

  Basu, Anupam ^a   Shrivastav, Tulsidas G ^a   Kariya, Kiran P ^b  

^a NATIONAL INSTITUTE OF HEALTH AND FAMILY WELFARE   (India)

^b Vardhamon Mahovir College   (India)

Author keywords

[No Author keywords available]

Indexed keywords

1 (3 DIMETHYLAMINOPROPYL) 3 ETHYLCARBODIIMIDE; ADIPIC ACID DERIVATIVE; ADIPIC ACID DIHYDRAZIDE; CARBOXYLIC ACID DERIVATIVE; CYANAMIDE; CYANOBOROHYDRIDE SODIUM; ENZYME; FUCOSE; HORSERADISH PEROXIDASE; HYDRAZONE DERIVATIVE; HYDROCORTISONE; HYDROCORTISONE SODIUM SUCCINATE; N ACETYLGLUCOSAMINE; STEROID; UNCLASSIFIED DRUG; XYLOSE;

ARTICLE; CONJUGATE; CROSS LINKING; DERIVATIZATION; ENZYME ACTIVITY; ENZYME IMMUNOASSAY; ENZYME LINKED IMMUNOSORBENT ASSAY; ENZYME MECHANISM; IMMUNOASSAY; OXIDATION; STEREOCHEMISTRY;

ADIPIC ACIDS; ANIMALS; ANTIBODIES; ENZYME-LINKED IMMUNOSORBENT ASSAY; HORSERADISH PEROXIDASE; HYDRAZINES; HYDROCORTISONE; INDICATORS AND REAGENTS; OXIDATION-REDUCTION; RABBITS;

ARMORACIA RUSTICANA;

EID: 0042868805     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1373/49.8.1410     Document Type: Article

References (16)

1. Dent AH, Aslam M. The preparation of protein-small molecule conjugate. In: Aslam M, Dent A, eds. Bioconjugation: protein coupling techniques for the biomedical sciences. London: Macmillan Reference Ltd., 1998:364-482.
2. Dent AH, Aslam M. Enzymes. In: Aslam M, Dent A, eds. Bioconjugation: protein coupling techniques for the biomedical sciences. London: Macmillan Reference Ltd., 1998:120.
3. Ornstein L. Discussion on "Enzyme-labeled antibodies for light and electron microscopic localization of antigens" by PK Nakane et al. J Histochem Cytochem 1966;14:790.
4. Nakane PK, Kawaoi A. Peroxidase-labeled antibody: a new method of conjugation. J Histochem Cytochem 1974;22:1084-91.
5. Boorsma DM, Streefekerk JG. Periodate or glutaraldehyde for preparing peroxidase conjugates? J Immunol Methods 1979;30:245-55.
6. Jeanson A, Cloes JM, Bouchet M, Rentier B. Comparison of conjugation procedure for the preparation of monoclonal antibody-enzyme conjugates. J Immunol Methods 1988;111:261-70.
7. Basu A, Shrivastav TG. One step enzyme linked immunosorbent assay for direct estimation of serum cortisol. J Immunoassay 2000;21:39-50.
8. Shrivastav TG, Pandey PK, Kumari GL. Enzyme immunosorbant assay of prolactin with penicillinase as label. Clin Chem 1988;34:2205-7.
9. O'Shannessy DJ, Wilchek M. Immobilization of glycoconjugates by their oligosaccharides: use of hydrazido-derivatized matrices. Anal Biochem 1990;191:1-8.
10. Satoh A, Kojima K, Koyama T, Ogawa H, Matsumoto I. Immobilization of saccharides and peptides on 96-well microtiter plates coated with methyl vinyl ether-maleic anhydride copolymer. Anal Biochem 1998;260:96-102.
11. Fleminger G, Hadas E, Wolf T, Solomon B. Oriented immobilization of periodate-oxidized monoclonal antibodies on amino and hydrazide derivatives of Eupergit C. Appl Biochem Biotechnol 1990;23:123-37.
12. Bayer EA, Ben-Hur H, Wilchek M. Avidin and streptavidin-containing probes. Methods Enzymol 1990;184:174-87.
13. Keren Z, Berke G, Gershoni JM. Identification of cell surface glycoproteins by periodate-alkaline phosphatase hydrazide. Anal Biochem 1986;155:182-7.
14. Dent AH, Aslam M. Enzymes. In: Aslam M, Dent A, eds. Bioconjugation: protein coupling techniques for the biomedical sciences. London: Macmillan Reference Ltd., 1998:121.
15. Schalkwijk J, van den Berg WB, van de Putte LBA, Joosten LAB, van den Bresselaar L. Cationization of catalase, peroxidase and superoxide dismutase. Effect of improved intraarticular retention on experimental arthritis in mice. J Clin Invest 1985;76:198-205.
16. Hermanson GT. Bioconjugate techniques. San Diego: Academic Press, 1996:121.