Purification, characterization and crystallization of a group of earthworm fibrinolytic enzymes from Eisenia fetida

Biotechnology Letters

Volumn 25, Issue 13, 2003, Pages 1105-1109

  Wang, Feng ^a   Wang, Chao ^a   Li, Mei ^a   Gui, Lulu ^a   Zhang, Jiping ^a   Chang, Wenrui ^a  

^a INSTITUTE OF BIOPHYSICS   (China)

Author keywords

Crystallization; Earthworm fibrinolytic enzyme; Purification; Substrate specificity

Indexed keywords

ENZYME INHIBITOR; FIBRINOLYTIC AGENT; PLASMINOGEN; SERINE PROTEINASE;

AMINO ACID SEQUENCE; ANIMAL; ANNELID WORM; ARTICLE; CHEMISTRY; CLASSIFICATION; COMPARATIVE STUDY; CRYSTALLIZATION; ENZYME SPECIFICITY; ISOLATION AND PURIFICATION; METHODOLOGY; MOLECULAR GENETICS; MOLECULAR WEIGHT;

AMINO ACID SEQUENCE; ANIMALS; CRYSTALLIZATION; ENZYME INHIBITORS; FIBRINOLYTIC AGENTS; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; OLIGOCHAETA; PLASMINOGEN; SERINE ENDOPEPTIDASES; SUBSTRATE SPECIFICITY;

ANIMALIA; ANNELIDA; EISENIA FETIDA; LUMBRICUS; LUMBRICUS RUBELLUS; PHERETIMA SIEBOLDI;

EID: 0042861787     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1024196232252     Document Type: Article

References (4)

1. Asturp T, Mullertz S (1952) The fibrin plate method for estimating fibrinolytic activity. Arch. Biochem. Biophys. 40: 346-351
2. Cong Yuwen, Liu Yaoming, Chen Jiapei (2001) The advance of lumbrokinase. Chin. J. Biochem. Pharm. 21: 159-162.
3. Mihara H, Sumi H, Yoneta T et al. (1991) A novel fibrinolytic enzyme extracted from the earthworm, Lumbricus rubellus. Jpn. J. Physiol. 41: 461-472.
4. Nakajima N, Mihara H, Sumi H (1993) Characterization of potent fibrinolytic enzymes in earthworm, Lumbricus rubellus. Biosci. Biotechnol. Biochem. 57: 1726-1730.