Flow cytometry - A simple method for nuclear DNA content evaluation of Vitis vinifera cv. Periquita somatic embryos obtained from anther cultures

Vitis

Volumn 42, Issue 2, 2003, Pages 99-100

  Lima, M A V A ^a   Paiva, A ^b   Candeias, M I ^a  

^a Estacao Agronomica Nacional   (Portugal)

^b Histocenter of Center   (Portugal)

Author keywords

DNA content; Flow cytometry; Somatic embryos; Vitis vinifera L.; Zygotic embryos

Indexed keywords

VITIS; VITIS SP.; VITIS VINIFERA;

EID: 0042850675     PISSN: 00427500     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (9)

1. ARUMUGANATHAN, K.; EARLE, E. D.; 1991 a: Nuclear DNA content of some important plant species. Plant Mol. Biol. Rep. 9, 208-218.
2. ARUMUGANATHAN, K.; EARLE, E. D.; 1991 b: Estimation of nuclear DNA content of plants by flow cytometry. Plant Mol. Biol. Rep. 9, 229-241.
3. D'AMATO, F.; 1991: Nuclear changes in cultured plant cells. Caryologia 44, 217-224.
4. FAURE, O.; NOUGARÈDE, A.; 1993: Nuclear DNA content of somatic and zygotic embryos of Vitis vinifera cv. Grenache noir at the torpedo stage. Protoplasma 176, 145-150.
5. LIMA, M. A.; CANDEIAS, M. I.; SEQUEIRA, O. A.; 1999: Cultura in vitro de anteras de castas Portuguesas de Vitis vinifera L.: indução de embriogénese somática e regeneração de plantas. In: Resumos 3° Workshop Biotecnologia de Plantas, Univ. UTAD, Vila Real.
6. LODHI, M. A.; REISCH, B. I.; 1995: Nuclear DNA content of Vitis species, cultivars, and other genera of the Vitaceae. Theor. Appl. Genet. 90, 11-16.
7. ORFAO, A.; GONZÁLEZ, M.; CIUDAD, J.; LÓPEZ-BERGES, M. C.; LÓPEZ, A.; VIDRIALES, B.; MACEDO, A.; SAN MIGUEL, J. F.; 1993: Cell cycle and DNA aneuploidy: Biological bases and terminology. In: A. SAMPEDRO, A. ORFAO (Eds): DNA Cytometric Analysis, 15-24. Univ. Oviedo, Oviedo.
8. RAJASEKARAN, K.; MULLINS, M. G.; 1983: The origin of embryos and plantlets from cultured anthers of hybrid grapevines. Am. J. Enol. Vitic. 34, 108-113.
9. WINKELMANN, T.; SANGWAN, R. S.; SCHWENKEL, H. G.; 1998: Flow cytometric analyses in embryogenic and non-embryogenic callus lines of Cyclamen persicum Mill.: Relation between ploidy level and competence for somatic embryogenesis. Plant Cell Rep. 17, 400-404.