Molecular cloning, gene organization and expression of the human UDP-GalNAc:Neu5Acα2-3Galβ-R β1,4-N-acetylgalactosaminyltransferase responsible for the biosynthesis of the blood group Sda/Cad antigen: Evidence for an unusual extended cytoplasmic domain

Biochemical Journal

Volumn 373, Issue 2, 2003, Pages 369-379

  Montiel, Maria Dolores ^a   Krzewinski Recchi, Marie Ange ^a   Delannoy, Philippe ^a   Harduin Lepers, Anne ^a  

^a UNIVERSITÉ DES SCIENCES ET TECHNOLOGIES DE LILLE   (France)

Author keywords

Molecular cloning; UDP GalNAc:Neu5Ac 2 3Gal R 1,4 N acetylgalactosaminyltransferase

Indexed keywords

ACIDS; CATALYSIS; CLONING; ENZYMES; GENES;

MOLECULAR CLONING;

BIOCHEMISTRY;

BETA 1,4 ACETYLGALACTOSAMINYLTRANSFERASE; BLOOD GROUP ANTIGEN; BLOOD GROUP CAD ANTIGEN; BLOOD GROUP SDA ANTIGEN; CHORIONIC GONADOTROPIN; N ACETYLGALACTOSAMINYLTRANSFERASE; TAMM HORSFALL GLYCOPROTEIN; UNCLASSIFIED DRUG; URIDINE DIPHOSPHATE N ACETYLGALACTOSE:5 ACETYLNEURAMINATE ALPHA 2,3 BETA GALACTOSE BETA 1,4 N ACETYLGALACTOSAMINYLTRANSFERASE;

ANIMAL CELL; ARTICLE; CELL STRAIN CACO 2; CELL STRAIN COS7; ENZYME ACTIVITY; ENZYME SUBSTRATE; GENE EXPRESSION REGULATION; GENETIC ORGANIZATION; GENETIC TRANSCRIPTION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; MOLECULAR CLONING; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN DOMAIN; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STOMACH MUCOSA;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; BLOOD GROUP ANTIGENS; BLOTTING, NORTHERN; CHROMOSOMES, HUMAN, PAIR 17; CLONING, MOLECULAR; GENE EXPRESSION REGULATION, ENZYMOLOGIC; HISTOCOMPATIBILITY ANTIGENS; HUMANS; MICE; MOLECULAR SEQUENCE DATA; N-ACETYLGALACTOSAMINYLTRANSFERASES; OLIGOSACCHARIDES; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; TRANSFECTION; TUMOR CELLS, CULTURED;

ANIMALIA; MAMMALIA;

EID: 0042847305     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20021892     Document Type: Article

References (55)

1. a. Vox Sang. 13, 485-492
2. a, a new blood group antibody. Vox Sang. 13, 493-501
3. a blood group antigen in tissues and body fluids. Vox Sang. 19, 472-482
4. a antigen in the human kidney and colon. Immunol. Invest. 17, 217-224
5. a-active pentasaccharide isolated from Tamm-Horsfall urinary glycoprotein. Biochem. Biophys. Res. Commun, 115, 625-631
6. Blanchard, D., Cartron, J. P., Fournet, B., Montreuil, J., van Halbeek, H. and Vliegenthart, J. F. (1983) Primary structure of the oligosaccharide determinant of blood group Cad specificity. J. Biol. Chem. 258, 7691-7695
7. a-antigen-like structures carried on core 3 are prominent features of glycans from the mucin of normal human descending colon. Biochem. J. 358, 657-664
8. M2 in gastric cancer and normal gastric mucosa. J Biol. Chem. 265, 7880-7785
9. Serafini-Cessi, F. and Dall'Olio, F. (1983) Guinea-pig kidney β-N-acetylgalactosaminyltransferase towards Tamm-Horsfall glycoprotein. Requirement of sialic acid in the acceptor for transferase activity. Bioohem. J. 215, 483-489
10. a antigen associated with Tamm-Horsfall glycoprotein. Carbohydr. Res. 151, 65-76
11. a antigen in human large intestine and colorectal carcinomas. Cancer Res. 49, 6466-6470
12. Piller, F., Blanchard, D., Huet, M. and Cartron, J. P. (1986) Identification of an α-NeuAc-(2-3)-β-D-galactopyranosyl N-acetyl-β-D-galactosaminyltransferase in human kidney. Carbohydr. Res. 149, 171-184
13. Takeya, A., Hosomi, O. and Kogure, T. (1987) Identification and characterization of UDP-GalNAc: NeuAcα2-3Galβ1-4Glc(NAc)β1-4 (GalNAc to Gal) N-acetylgalactosaminyltransferase in human blood plasma. J. Biochem. (Tokyo) 101, 251-259
14. a+ individuals. Arch. Biochem. Biophys. 266, 573-582
15. Dall'Olio, F., Malagolini, N. and Serafini-Cessi, F. (1987) Tissue distribution and age-dependent expression of β-4-N-acetylgalactosaminyl-transferase in guinea-pig. Biosci. Rep. 7, 925-932
16. a antigen of α2,6-sialyltransferase activity towards N-acety-llactosamine. Biochem. J. 270, 519-524
17. a specificity in human colon carcinoma Caco-2 cell line. Biochem. Biophys. Res. Commun. 180, 681-686
18. Smith, P. L. and Lowe, J. B. (1994) Molecular cloning of a murine N-acetyl-galactosamine transferase cDNA that determines expression of the T lymphocyte-specific CT oligosaccharide differentiation antigen. J. Biol. Chem. 269, 15162-15171
19. Rencurosi, A., Poletti, L., Guerrini, M., Russo, G. and Lay, L. (2002) Human milk oligosaccharides: an enzymatic protection step simplifies the synthesis of 3′- and 6′-O-sialyllactose and their analogues. Carbohydr. Res. 337, 473-483
20. Pinto, M., Robine-Leon, S., Appay, M. D., Kedinger, M., Triadou, N., Dussaulx, E., Lacroix, B., Simon-Assmann, P., Haffen, K., Fogh, J. and Zweibaum, A. (1983) Enterocyte-like differentiation and polarization of the human colon carcinoma cell line Caco-2 in culture. Biol. Cell. 47, 323-330
21. Altschul, S. F., Madden, T. L., Schäffer, A. A., Zhang, J., Miller, W. and Lipman, D. J. (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25, 3389-3402
22. Hofmann, K. and Stoffel, W. (1993) A database membrane spanning protein segments. Biol. Chem. Hoppe-Seyler 347, 166
23. Hansen, J. E., Lund, O., Tolstrup, N., Gooley, A. A., Williams, K. L. and Brunak, S. (1998) NetOglyc: prediction of mucin type O-glycosylation sites based on sequence context and surface accessibility. Glycoconj. J. 15, 115-130
24. Sanger, F., Nicklen, S. and Coulsen, A. R. (1977) DNA sequencing with chain terminating inhibitors. Proc. Natl. Acad. Sci. U.S.A. 74, 5463-5467
25. Zanetta, J. P., Gouyer, V., Maes, E., Pons, A., Hemon, B., Zweibaum, A., Delannoy, P. and Huet, G. (2000) Massive in vitro synthesis of tagged oligosaccharides in 1-benzyl-2-acetamido-2deoxy-α-D-galactopyranoside treated HT-29 cells. Glycobiology 10, 565-575
26. Recchi, M. A., Hebbar, M. Hornez, L., Harduin-Lepers, A., Peyrat, J. P. and Delannoy, P. (1998) Multiplex reverse transcription polymerase chain reaction assessment of sialyltransferase expression in human breast cancer. Cancer Res. 58, 1066-4070
27. a blood group carbohydrate structure in human gastrointestinal mucosa and cancer. Int. J. Cancer. 67, 626-631
28. a-β1,4-N-acetylgalactosaminyl-transferase is released by differentiated human colon carcinoma Caco-2 cells. Glycoconj. J. 12, 773-779
29. Kozak, M. (1992) Regulation of translation in eukaryotic systems. Annu. Rev. Cell Biol. 8, 197-225
30. Coutinho, P. M. and Henrissat, B. (1999) Carbohydrate-active enzymes: an integrated database approach. In Recent Advances in Carbohydrate Bioengineering (Gilbert, H. J., Davies, G., Henrissat, B. and Svensson, B., eds.), pp. 3-12, The Royal Society of Chemistry, Cambridge
31. Shapiro, M. B. and Senapathy, P. (1987) RNA splice junctions of different classes of eukaryotes: sequence statistics and functional implications in gene expression. Nucleic Acids Res. 15, 7155-7174
32. Reference deleted
33. Lefrançois, L. and Bevan, M. J. (1985) Functional modifications of cytotoxic T-lymphocyte T200 glycoprotein recognized by monoclonal antibodies. Nature (London) 314, 449-452
34. Conzelmann, A. and Kornfeld, S. (1984) β-Linked N-acetylgalactosamine residues present at the nonreducing termini of O-linked oligosaccharides of a cloned murine cytotoxic T lymphocyte line are absent in a Vicia villosa lectin-resistant mutant cell line. J. Biol. Chem. 259, 12528-12535
35. Conzelmann, A. and Bron, C. (1987) Expression of UDP-N-acetylgalactosamine: β-galactose β1,4-N-acetylgalactosaminyltransferase in functionally defined T-cell clones. Biochem. J. 242, 817-824
36. Shaper, N. L., Hollis, G. F., Douglas, J. G., Kitsch, I. R. and Shaper, J. H. (1988) Characterization of the full-length cDNA for murine β-1,4-galactosyltransferase. Novel features at the 5′-end predict two translational start sites at two in-frame AUGs. J. Biol. Chem. 263, 10420-10428
37. Russo, R. N., Shaper, N. L. and Shaper, J. H. (1990) Bovine β1,4-galactosyltransferase: two sets of mRNA transcripts encode two forms of the protein with different amino-terminal domains. In vitro translation experiments demonstrate that both the short and the long forms of the enzyme are type II membrane-bound glycoproteins. J. Biol. Chem. 265, 3324-3331
38. Wen, D. X., Svensson, E. C. and Paulson J. C. (1992) Tissue-specific alternative splicing of the β-galactoside α2,6-sialyltransferase gene. J. Biol. Chem. 267, 2512-2518
39. Aasheim, H. C., Aas-Eng, D. A., Deggerdal, A., Blomhoff, H. K., Funderud, S. and Smeland, E. B. (1993) Cell-specific expression of human β-galactoside α2,6-sialyltransferase transcripts differing in the 5′ untranslated region. Eur. J. Biochem. 213, 467-475
40. Wang, X., Vertino, A., Eddy, R. L., Byers, M. G., Jani-Sait, S. N., Shows, T. B. and Lau, J. T. (1993) Chromosome mapping and organization of the human β-galactoside α2,6-sialyltransferase gene. Differential and cell-type specific usage of upstream exon sequences in B-lymphoblastoid cells. J. Biol. Chem. 268, 4355-4361
41. Kitagawa, H. and Paulson, J. C. (1994) Cloning of a novel α2,3-sialyltransferase that sialylates glycoprotein and glycolipid carbohydrate groups. J. Biol. Chem. 269, 1394-1401
42. Furukawa, K., Soejima, H., Niikawa, N. and Shiku, H. (1996) Genomic organization and chromosomal assignment of the human β1,4-N-acetylgalactosaminyltransferase gene. Identification of multiple transcription units. J. Biol. Chem. 271, 20836-20844
43. Paulson, J. C., Weinstein, J. and Schauer, A. (1989) Tissue-specific expression of sialyltransferases. J. Biol. Chem. 264, 10931-10934
44. Berger, E. G. (2002) Ectopic localizations of Golgi glycosyltransferases. Glycobiology 12, 29-36
45. Gelman, I. H., Lee, K., Tombler, E., Gordon, R. and Lin, X. (1998) Control of cytoskeletal architecture by src-supressed C kinase substrate, SSeCKS. Cell Motil. Cytoskel. 41, 1-17
46. Wassler, M. J., Foote, C. I., Gelman, I. H. and Shur, B. D. (2001) Functional interaction between the SSeCKS scaffolding protein and the cytoplasmic domain of β1,4-galactosyltransferase. J. Cell Sci. 114, 229-2300
47. D2 gangliosides J. Biol. Chem. 267, 12082-12089
48. Breton, C., Bettler, E., Joziasse, D. H., Geremia, R. A. and Imberty, A. (1998) Sequence-function relationships of prokariotic and eukariotic galactosyltransferases. J. Biochem. (Tokyo) 123, 1000-1009
49. Wiggins, C. A. R. and Munro, S. (1998) Activity of the yeast MNN1 α-1,3-mannosyltransferase requires a motif conserved in many other families of glycosyltransferases. Proc. Natl. Acad. Sci. U.S.A 95, 7945-7950
50. Gastinel, L. N., Cambillau, C. and Bourne, Y. (1999) Crystal structures of the bovine β4galactosyltransferase catalytic domain and its complex with uridine diphosphogalactose. EMBO J. 18, 3546-3557
51. M2 synthase activity but is not critical for nucleotide binding. Glycobiology 11, 217-229
52. Kitagawa, H., Uyama, T. and Sugahara, K. (2001) Molecular cloning and expression of a human chondroitin synthase. J. Biol. Chem. 276, 38721-38726
53. Uyama, T., Kitagawa, H., Tamura, Ji. J. and Sugahara, K. (2002) Molecular cloning and expression of human chondroitin N-acetylgalactosaminyltransferase: the key enzyme for chain initiation and elongation of chondroitin/dermatan sulfate on the protein linkage region tetrasaccharide shared by heparin/heparan sulfate. J. Biol. Chem. 277, 8841-8846
54. Meurer, J. A., Naylor, J. M., Baker, C. A., Thomsen, D. R., Homa, F. L. and Elhammer, A. P. (1995) cDNA cloning, expression, and chromosomal localization of a human UDP-GalNAc:polypeptide, N-acetylgalactosaminyltransferase. J. Biochem. (Tokyo) 118, 568-574
55. Gotoh, M., Yada, T., Sato, T., Akashima, T., Iwasaki, H., Mochizuki, H., Inaba, N., Togayachi, A., Kudo, T., Watanabe, H., Kimata, K. and Narimatsu, H. (2002) Molecular cloning and characterization of a novel chondroitin sulfate glucuronyltransferase that transfers glucuronic acid to N-acetylgalactosamine. J. Biol. Chem. 277, 38179-38188