Transposon-5 mutagenesis transforms Corynebacterium matruchotii to synthesize novel hybrid fatty acids that functionally replace corynomycolic acid

Biochemical Journal

Volumn 373, Issue 2, 2003, Pages 465-474

  Takayama, Kuni ^a,b,c   Hayes, Barry ^a   Vestling, Martha M ^c   Massey, Randall J ^b  

^a WILLIAM S MIDDLETON MEMORIAL VETERANS HOSPITAL   (United States)

^b UNIVERSITY OF WISCONSIN SCHOOL OF MEDICINE AND PUBLIC HEALTH   (United States)

^c UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Claisen type condensation; Corynomycolic acid; Electron impact MS; Electrospray MS; Transposon 5 mutagenesis; Ultrathin section electron microscopy

Indexed keywords

BIOSYNTHESIS; CONDENSATION; DRUG DOSAGE; FATTY ACIDS;

MYCOLIC ACID;

ENZYMES;

CHLOROFORM; CORYNOMYCOLIC ACID; FATTY ACID; GLYCOLIPID; LYSOZYME; METHANOL; MYCOLIC ACID; TREHALOSE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIUM MUTANT; CHEMICAL ANALYSIS; CHEMICAL BOND; CLAISEN CONDENSATION; CONTROLLED STUDY; CORYNEBACTERIUM; CORYNEBACTERIUM MATRUCHOTII; FATTY ACID SYNTHESIS; MASS SPECTROMETRY; MUTAGENESIS; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PRIORITY JOURNAL; SAPONIFICATION; TEMPERATURE SENSITIVITY; TRANSPOSON; WILD TYPE;

ACETYLATION; ANTI-BACTERIAL AGENTS; CHLOROFORM; CORYNEBACTERIUM; DNA TRANSPOSABLE ELEMENTS; DRUG RESISTANCE, BACTERIAL; FATTY ACIDS; KANAMYCIN; METHANOL; MODELS, CHEMICAL; MUTAGENESIS; MUTATION; MYCOLIC ACIDS; OXIDATION-REDUCTION; PALMITIC ACIDS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TREHALOSE;

BACTERIA (MICROORGANISMS); CORYNEBACTERIUM; CORYNEBACTERIUM MATRUCHOTII; MYCOBACTERIUM; MYCOBACTERIUM TUBERCULOSIS; TRANSPOSONS;

EID: 0042847298     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20030248     Document Type: Article

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