Rapid isolation of Arabidopsis thaliana developing embryos

BioTechniques

Volumn 35, Issue 2, 2003, Pages 278-282

  Perry, Sharyn E ^a   Wang, Huai ^a  

^a UNIVERSITY OF KENTUCKY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARABIDOPSIS; ARTICLE; COTYLEDON; DENATURATION; EMBRYO DEVELOPMENT; EXTRACTION; FREEZING; GENE EXPRESSION; METHODOLOGY; NONHUMAN; PLANT GENETICS; PLANT GROWTH; PLANT REPRODUCTION; PLANT SEED; POLYMERASE CHAIN REACTION; PROTEIN ISOLATION; RNA ISOLATION; ROOM TEMPERATURE;

ARABIDOPSIS; ARABIDOPSIS THALIANA;

EID: 0042736181     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/03352bm06     Document Type: Article

References (9)

1. Ruuska, S.A. and J.B. Ohlrogge. 2001. Protocol for small-scale RNA isolation and transcriptional profiling of developing Arabidopsis seeds. BioTechniques 31:752-758.
2. Wilkins, T.A. and L.B. Smart. 1996. Isolation of RNA from plant tissue, p. 21-41. In P.A. Krieg (Ed.), A Laboratory Guide to RNA: Isolation, Analysis, and Synthesis. Wiley-Liss, New York.
3. Ito, T., N. Takahashi, Y. Shimura, and K. Okada. 1997. A serine/ threonine protein kinase gene isolated by an in vivo binding procedure using the Arabidopsis floral homeotic gene product, AGAMOUS. Plant Cell Physiol. 38:248-258.
4. Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15:473-497.
5. Wang, H., W. Tang, C. Zhu, and S.E. Perry. 2002. A chromatin immunoprecipitation (ChIP) approach to isolate genes regulated by AGL15, a MADS domain protein that preferentially accumulates in embryos. Plant J. 32:831-843.
6. Heck, G.R., S.E. Perry, K.W. Nichols, and D.E. Fernandez. 1995. AGL15, a MADS-domain protein expressed in developing embryos. Plant Cell 7:1271-1282.
7. Rounsley, S.D., G.S. Ditta, and M.F. Yanofsky. 1995. Diverse roles for MADS-box genes in Arabidopsis development. Plant Cell 7:1259-1269.
8. Snustad, D.P., N.A. Haas, S.D. Kopczak, and C.D. Silflow. 1992. The small genome of Arabidopsis contains at least nine expressed β-tubulin genes. Plant Cell 4:549-556.
9. Axelos, M., C. Bardet, T. Liboz, A.L.V. Thai, C. Curie, and B. Lescure. 1989. The gene family encoding the Arabidopsis thaliana translation elongation factor EF-1 α: molecular cloning, characterization, and expression. Mol. Gen. Genet. 219:106-112.