Improved PCR amplification of multiple specific alleles (PAMSA) using internally mismatched primers

BioTechniques

Volumn 21, Issue 1, 1996, Pages

  Okimoto, Ronald ^a   Dodgson, Jerry B ^a,b  

^a MICHIGAN STATE UNIVERSITY   (United States)

^b Michigan State University ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PRIMER DNA;

ALLELE; ARTICLE; BASE PAIRING; DNA SEQUENCE; GENE AMPLIFICATION; GENE MUTATION; NONHUMAN; POLYMERASE CHAIN REACTION;

EID: 0042712609     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96211bm03     Document Type: Article

References (9)

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2. Crittenden, L.B., L. Provencher, L. Santangelo, I. Levin, H. Abplanalp, R.W. Briles, W.E. Briles and J. B. Dodgson. 1993. Characterization of a red jungle fowl by white leghorn backcross reference population for molecular mapping of the chicken genome. Poult. Sci. 72:334-348.
3. Dutton, C. and S.S. Sommer. 1991. Simultaneous detection of multiple single-base alleles at a polymorphic site. BioTechniques 11:700-702.
4. Levin, I., L.B. Crittenden and J.B. Dodgson. 1994. Polymorphic DNA mapping using avian CR1 element-derived PCR primers. J. Hered. 85:73-78.
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7. Sarkar, G., J. Cassady, C.D.K. Bottema and S.S. Sommer. 1990. Characterization of polymerase chain reaction amplification of specific alleles. Anal. Biochem. 186:64-68.
8. Sommer, S.S., A.R. Groszbach and C.D.K. Bottema. 1992. PCR amplification of specific alleles (PASA) is a general method for rapidly detecting known single-base changes. BioTechniques 12:82-87.
9. Wu, D.Y., L. Ugozzoli, B.K. Pal and R.B. Wallace. 1989. Allele-specific enzymatic amplification of β-globin genomic DNA for diagnosis of sickle cell anemia. Proc. Natl. Acad. Sci. USA 86:2757-2760.