A highly sensitive flow-through amperometric immunosensor based on the Peroxidase chip and enzyme-channeling principle

Biosensors and Bioelectronics

Volumn 18, Issue 11, 2003, Pages 1321-1327

  Zeravik, J ^a   Ruzgas, T ^b   Franek M ^a  

^a VETERINARY RESEARCH INSTITUTE   (Czech Republic)

^b LUND UNIVERSITY   (Sweden)

Author keywords

Antibody; Co immobilization; Enzyme channeling; Glucose oxidase; Immunosensor; Recombinant peroxidase

Indexed keywords

ELECTROCHEMICAL ELECTRODES; ENVIRONMENTAL IMPACT; ENZYMES; GOLD;

ENVIRONMENTAL POLLUTANTS;

IMMUNOSENSORS;

GOLD; HERBICIDE; PEROXIDASE; TRIAZINE DERIVATIVE;

AMPEROMETRY; ARTICLE; ASPERGILLUS NIGER; BINDING SITE; BIOSENSOR; CONTROLLED STUDY; ELECTRODE; ELECTRON TRANSPORT; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; GENETIC ENGINEERING; IC 50; IMMUNOSENSOR; NONHUMAN; POLLUTION MONITORING; SIGNAL TRANSDUCTION;

EID: 0042706515     PISSN: 09565663     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0956-5663(03)00076-9     Document Type: Article

References (11)

1. Appelqvist R., Marko-Varga G., Gorton L., Torstensson A., Johansson G. Enzymatic determination of glucose in a flow system by catalytic-oxidation of the nicotinamide coenzyme at a modified electrode. Anal. Chim. Acta. 169:1985;237-247.
2. 2 based on recombinant forms of horseradish peroxidase directly adsorbed on polycrystalline gold. Biosens. Bioelectron. 16:2001;147-157.
3. Fránek M., Kolar V., Eremin S.A. Enzyme immunoassays for s-triazine herbicides and their application in environmental and food analysis. Anal. Chim. Acta. 311:1995;349-356.
4. Grigorenko V., Chubar T., Kapeliuch Y., Börchers T., Spener F., Egorov A. New approaches for functional expression of recombinant horseradish peroxidase C in Escherichia coli. Biocatal. Biotransform. 17:1999;359-379.
5. Hoare J.P. A cyclic voltammetric study of the gold-oxygen system. J. Electrochem. Soc. 131:1984;1808-1815.
6. Ivnitski D., Rishpon J. A one-step, separation-free amperometric enzyme immunosensor. Biosens. Bioelectron. 11:1996;409-417.
7. Keay R.W., McNeil C.J. Separation-free electrochemical immunosensor for rapid determination of atrazine. Biosens. Bioelectron. 13:1998;963-970.
8. Litman D.J., Hanlon T.M., Ullman E.F. Enzyme channeling immunoassay - A new homogeneous enzyme-immunoassay techique. Anal. Biochem. 106:1980;223-229.
9. Presnova G., Grigorenko V., Egorov A., Ruzgas T., Lindgren A., Gorton L., Börchers T. Direct heterogenous electron transfer of recombinant horseradish peroxidases on gold. Faraday Discuss. 116:2000;281-289.
10. Rishpon J., Ivnitski D. An amperometric enzyme-channeling immunosensor. Biosens. Bioelectron. 12:1997;195-204.
11. Skladal P. Advances in electrochemical immunosensors. Electroanal. 9:1997;737-745.