Quantitative fluorescent speckle microscopy: Where it came from and where it is going

Journal of Microscopy

Volumn 211, Issue 3, 2003, Pages 191-207

  Danuser, Gaudenz ^a,b   Waterman Storer, C M ^b  

^a ETH ZURICH   (Switzerland)

^b SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

Cytoskeleton; Fluorescent speckle microscopy; Polymer

Indexed keywords

CELLS; CYTOLOGY; DIAGNOSIS; DYNAMICS; FLUORESCENCE; PROTEINS;

BINDING PROTEINS; CELL MIGRATION; CYTOSKELETAL DYNAMICS; CYTOSKELETONS; F-ACTIN; FLUORESCENT SPECKLE MICROSCOPY; MICROTUBULES; PHOTOMETRICS; POLYMER TRANSPORT; SPECIALIZED SOFTWARE;

SPECKLE;

BINDING PROTEIN; F ACTIN;

ACCURACY; AUTOMATION; CELL DIVISION; CELL MIGRATION; CELL STRUCTURE; CHEMICAL STRUCTURE; COMPUTER PROGRAM; CYTOSKELETON; FLUORESCENT SPECKLE MICROSCOPY; IMAGE ANALYSIS; IMAGING SYSTEM; MACROMOLECULE; MEDICAL TECHNOLOGY; MICROSCOPY; MICROTUBULE; MORPHOGENESIS; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REVIEW;

EID: 0042412347     PISSN: 00222720     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2818.2003.01222.x     Document Type: Review

References (72)

1. Adams, M.C., Salmon, W.C., Gupton, S.L., Cohan, C.S., Wittmann, T., Prigozhina, N. & Waterman-Storer, C.M. (2003) A high-speed multi-spectral spinning disc confocal microscope system for fluorescent speckle microscopy of living cells. Methods, 29, 29-41.
2. Amann, K.J. & Pollard, T.D. (2001) Direct real-time observation of actin filament branching mediated by Arp2/3 complex using total internal reflection fluorescence microscopy. Proc. Natl Acad. Sci. USA. 98, 15009-15013.
3. Axelrod, D. (2001) Total internal reflection microscopy in cell biology. Traffic, 2, 764-774.
4. Brust-Mascher, I. & Scholey, J.M. (2002) Microtubule flux and sliding in mitotic spindles of Drosophila embryos. Mol. Biol. Cell. 13, 3967-3975.
5. Bulinski, J.C., Odde, D.J., Howell, B.J., Salmon, E.D. & Waterman-Storer, C.W. (2001) Rapid dynamics of the microtubule binding of ensconsin in vivo. J. Cell Sci. 114, 3885-3897.
6. Chang, S., Svitkina, T.M., Borisy, G.G. & Popov, S.V. (1999) Speckle microscopic evaluation of microtubule transport in growing nerve processes. Nat. Cell Biol. 1, 399-403.
7. Danuser, G. & Mazza, E. (1996) Observing deformations of 20 nanometer with a low numerical aperture light microscope. Optical Inspection and Micromeasurements, (Ed. Gorecki, C.) Vol. 2782, pp. 180-191. Proc. of SPIE Becançon, France.
8. Danuser, G., Tran, P.T. & Salmon, E.D. (2000) Tracking differential interference contrast diffraction line images with nanometre sensitivity. J. Microsc. 198, 34-53.
9. Dent, E.W., Callaway, J.L., Szebenyi, G., Baas, P.W. & Kalil, K. (1999) Reorganization and movement of microtubules in axonal growth cones and developing interstitial branches. Neuroscience, 19, 8894-8908.
10. Dent, E.W. & Kalil, K. (2001) Axon branching requires interactions between dynamic microtubules and actin filaments. J. Neurosci. 15, 9757-9769.
11. Desai, A. & Mitchison, T.J. (1997) Microtubule polymerization dynamics. Ann. Rev. Cell Dev. Biol. 13, 83-117.
12. Dufort, P.A. & Lumsden, C. (1993) Cellular automation model of the actin cytoskeleton. Cell Motil. Cytoskeleton, 25, 87-104.
13. Dunn, G.A., Dobbie, I.M., Monypenny, J., Holt, M.R. & Zicha, D. (2002) Fluorescence localization after photobleaching (FLAP): a new method for studying protein dynamics in living cells. J. Microsc. 205, 109-112.
14. Galbiati, F., Razani, B. & Lisanti, M.P. (2001) Emerging themes in lipid rafts and caveolae. Cell, 106, 403-411.
15. Gasser, S.M. (2002) Visualizing chromatin dynamics in interphase nuclei. Science, 296, 1412-1416.
16. Geiger, B., Bershadsky, A., Pankov, R. & Yamada, K.M. (2001) Transmembrane extracellular matrix-cytoskeleton crosstalk. Nature Rev. 2, 793-805.
17. Grego, S., Cantillana, V. & Salmon, E.D. (2001) Microtubule treadmilling in vitro investigated by fluorescence speckle and confocal microscopy. Biophys. J. 81, 66-78.
18. Gupton, S.L., Salmon, W.C. & Waterman-Storer, C.M. (2002) Converging populations of f-actin promote breakage of associated microtubules to spatially regulate microtubule turnover in migrating cells. Curr. Biol. 12, 1891-1899.
19. Harris, A.K. (1999) A dozen questions about how tissue cells crawl. Biochem. Soc. Symp. 65, 315-341.
20. Hollenbeck, P.J. & Bamburg, J.R. (1999) Axonal microtubules stay put. Nat. Cell Biol. 1, E171-E173.
21. Inoué, S. & Spring, K. (1997) Video Microscopy: the Fundamentals. 2nd edn. Plenum Press, New York, NY.
22. Kabir, N., Schaefer, A.W., Nakhost, A., Sossin, W.S. & Forscher, P. (2001) Protein kinase C activation promotes microtubule advance in neuronal growth cones by increasing average microtubule growth lifetimes. J. Cell Biol. 5, 1033-1044.
23. Kapoor, T.M. & Mitchison, T.J. (2001) Eg5 is static in bipolar spindles relative to tubulin: evidence for a static spindle matrix. J. Cell Biol. 154, 1125-1133.
24. Karsenti, E. & Vernos, I. (2001) The mitotic spindle: a self-made machine. Science, 294, 543-547.
25. Leonhardt, H., Sporbert, A. & Cardoso, M.C. (2000) Targeting regulatory factors to intranuclear replication sites. Crit. Rev. Eukaryot. Gene Expr. 10, 127-133.
26. Lyman, S.K. & Gerace, L. (2001) Nuclear pore complexes: dynamics in unexpected places. J. Cell Biol. 154, 17-20.
27. Maddox, P.S., Bloom, K.S. & Salmon, E.D. (2000) The polarity and dynamics of microtubule assembly in the budding yeast Saccharomyces cerevisiae. Nature Cell Biol. 2, 36-41.
28. Maddox, P., Chin, E., Mallavarapu, A., Yeh, E., Salmon, E.D. & Bloom, K. (1999) Microtubule dynamics from mating through the first zygotic division in the budding yeast Saccharomyces cerevisiae. J. Cell Biol. 144, 977-987.
29. Maddox, P., Desai, A., Oegema, K., Mitchison, T.J. & Salmon, E.D. (2002) Polewards microtubule flux in a major component of anaphase-A in mitotic Drosophila embryos. Curr. Biol. 12, 1670-1674.
30. Maddox, P., Straight, A.F., Coughlin, P., Mitchison, T.J. & Salmon, E.D. (2003) Direct observation of microtubule polymerization/depolymerization at kinetochores in Xenopus extract spindles: towards a unified view of spindle mechanics. J. Cell Biol., in press.
31. McDonnell, J.M. (2001) Surface plasmon resonance: towards an understanding of the mechanisms of biological molecular recognition. Curr. Opin. Chem. Biol. 5, 572-577.
32. Mikhailov, A.V. & Gundersen, G.G. (1995) Centripetal transport of microtubules in motile cells. Cell Motil. Cytoskeleton, 32, 173-186.
33. Mitchison, T.J. (1989) Polewards microtubule flux in the mitotic spindle: evidence from photoactivation of fluorescence. J. Cell Biol. 109, 637-652.
34. Oshiro, M. (1998) Cooled CCD versus intensified cameras for low-light video - applications and relative advantages. Video Microscopy (ed. by G. Sluder & D. Wolf). pp. 45-62. Academic Press, San Diego.
35. Perez, F., Diamantopoulos, G.S., Stalder, R. & Kreis, T.E. (1999) CLIP-170 highlights growing microtubule ends in vivo. Cell, 96, 517-527.
36. Periasamy, A. (2001) Fluorescence resonance energy transfer microscopy: a mini review. J. Biomed. Opt. 6, 287-291.
37. Perlman, Z.E., Kapoor, T.M. & Mitchison, T.J. (2001) Automated analysis of flux in fluorescent speckled mitotic spindles. Biophys. J. 80, 2157.
38. Pollard, T.D. (1986) Rate constants for the reactions of ATP-actin and ADP-actin with the ends of actin filaments. J. Cell Biol. 103, 2747-2754.
39. Pollard, T.D., Blanchoin, L. & Mullins, R.D. (2000) Molecular mechanisms controlling actin filament dynamics in nonmuscle cells. Annu. Rev. Biophys. Biomol. Struct. 29, 545-576.
40. Ponti, A., Vallotton, P., Salmon, W.C., Waterman-Storer, C.M. & Danuser, G. (2003) Computational analysis of f-actin turnover in cortical actin meshworks using fluorescent speckle microscopy. Biophys. J. 84, 3336-3352.
41. Prasher, D.C. (1995) Using GFP to see the light. Trends Genet. 11, 320-323.
42. Salmon, W.C., Adams, M.C. & Waterman-Storer, C.M. (2002) Dual-wavelength fluorescent speckle microscopy reveals coupling of microtubule and actin movements in migrating cells. J. Cell Biol. 158, 31-37.
43. Schaefer, A.W., Kabir, N. & Forscher, P. (2002) Filopodia and transverse retrograde f-actin arcs guide the assembly and transport of two populations of microtubules with unique dynamic parameters in neuronal growth cone. J. Cell Biol. 158, 139-152.
44. Schafer, D.A. (2002) Coupling actin dynamics and membrane dynamics during endocytosis. Curr. Opin. Cell Biol. 14 (1), 76-81.
45. Sedgewick, R. (2002) Algorithms in C++ - Part 5 Graph Algorithms. Addison-Wesley, New York.
46. Small, V. (1981) Organization of actin in the leading edge of cultured cells. J. Cell Biol. 91, 695-705.
47. Svitkina, T.M., Verkhovsky, A.B., McQuade, K.M. & Borisy, G.G. (1997) Analysis of the actin-myosin II system in fish epidermal keratocytes. Mechanism of cell body translocation. J. Cell Biol. 139, 397-415.
48. Theriot, J.A. & Mitchison, T.J. (1991) Actin microfilament dynamics in locomoting cells. Nature, 352, 126-131.
49. Toomre, D. & Manstein, D.J. (2001) Lighting up the cell surface with evanescent wave microscopy. Trends Cell Biol. 11, 298-303.
50. Tran, P.T., Marsh, L., Doye, V., Inoue, S. & Chang, F. (2001) A mechanism for nuclear positioning in fission yeast based on microtubule pushing. J. Cell Biol. 153, 397-411.
51. Vallotton, P., Ponti, A., Salmon, E.D., Waterman-Storer, C.M. & Danuser, G. (2003) Recovery, visualization, and analysis of actin and tubulin polymer flow in live cells: a fluorescent speckle microscopy study. Biophys. J. 85, 1289-1306.
52. Verkhovsky, A.B., Svitkina, T.M. & Borisy, G.G. (1999) Self-polarization and directional motility of cytoplasm. Curr. Biol. 9, 11-20.
53. Vorobjev, I., Malikov, V. & Rodionov, V. (2001) Self-organization of a radial microtubule array by dynein-dependent nucleation of microtubules. Proc. Natl Acad. Sci. USA, 98, 10160-10165.
54. Wachman, E.S., Niu, W. & Farkas, D.L. (1997) AOTF microscope for imaging with increased speed and spectral versatility. Biophys. J. 73, 1215-1222.
55. Wadsworth, P. & Salmon, E.D. (1986) Analysis of the treadmilling model during metaphase of mitosis using fluorescence redistribution after photobleaching. J. Cell Biol. 102, 1032-1038.
56. Wang, Y.L. (1985) Exchange of actin subunits at the leading edge of living fibroblasts: possible role of treadmilling. J. Cell Biol. 101, 597-602.
57. Wang, Y.L., Heiple, J.M. & Taylor, D.L. (1982) Fluorescent analog cytochemistry of contractile proteins. Methods Cell Biol. 25, 1-11.
58. Watanabe, N. & Mitchison. T.J. (2002) Single-molecule speckle analysis of actin filament turnover in lamellipodia. Science, 295, 1083-1086.
59. Waterman-Storer, C.M. (2002) Fluorescent speckle microscopy (FSM) of microtubules and actin in living cells. Current Protocols in Cell Biology (ed. by J. S. Bonifacino, M. Dasso, J. B. Harford, J. Lippincott-Schwartz & K. M. Yamada), Unit 4-10. John Wiley, New York.
60. Waterman-Storer, C.M. & Danuser, G. (2002) New directions for fluorescent speckle microcopy. Curr. Biol. 12, R633-R640.
61. Waterman-Storer, C.M., Desai, A., Bulinski, J.C. & Salmon, E.D. (1998) Fluorescent speckle microscopy: visualizing the movement, assembly, and turnover of macromolecular assemblies in living cells. Curr. Biol. 8, 1227-1230.
62. Waterman-Storer, C.M., Desai, A. & Salmon, E.D. (1999) Fluorescent speckle microscopy of spindle microtubule assembly and motility in living cells. Methods Cell. Biol. 61, 155-173.
63. Waterman-Storer, C.M., Duey, D.Y., Weber, K.L., Keech, J., Cheney, R.E., Salmon, E.D. & Bement, W.M. (2000a) Microtubules remodel actomyosin networks in Xenopus egg extracts via two mechanisms of F-actin transport. J. Cell Biol. 150, 361-376.
64. Waterman-Storer, C.M, & Salmon, E.D. (1997) Actomyosin-based retrograde flow of microtubules in the lamella of migrating epithelial cells influences microtubule dynamic instability and turnover and is associated with microtubule breakage and treadmilling. J. Cell Biol. 139, 417-434.
65. Waterman-Storer, C.M. & Salmon, E.D. (1998) How microtubules get fluorescent speckles. Biophys. J. 75, 2059-2069.
66. Waterman-Storer, C.M. & Salmon, E.D. (1999) Fluorescent speckle microscopy of microtubules: how low can you go? FASEB J. 13, S225-S230.
67. Waterman-Storer, C.M., Salmon, W.C. & Salmon, E.D. (2000b) Feedback interactions between cell-cell adherens junctions and cytoskeletal dynamics in newt lung epithelial cells. Mol. Biol. Cell. 11, 2471-2483.
68. Waterman-Storer, C.M., Sanger, J.W. & Sanger, J.M. (1993) Dynamics of organelles in the mitotic spindles of living cells: membrane and microtubule interactions. Cell Motil. Cytoskeleton, 26, 19-39.
69. Wittmann, T., Hyman, A. & Desai, A. (2001) The spindle: a dynamic assembly of microtubules and motors. Nat. Cell Biol. 3, E28-E34.
70. Wolf, D.E. (1989) Designing, building, and using a fluorescence recovery after photobleaching instrument. Methods Cell Biol. 30, 271-306.
71. Zhou, F.Q., Waterman-Storer, C.M. & Cohan, C.S. (2002) Focal loss of actin bundles causes microtubule redistribution and growth cone turning. J. Cell Biol. 157, 839-849.
72. Zicha, D., Dobbie, I.M., Holt, M.R., Monypenny, J., Soong, D.Y.H., Gray, C. & Dunn, G.A. (2003) Rapid actin transport during cell protrusion. Science. 300, 142-145.