Thin pilus PilV adhesins of plasmid R64 recognize specific structures of the lipopolysaccharide molecules of recipient cells

Journal of Bacteriology

Volumn 185, Issue 17, 2003, Pages 5192-5199

  Ishiwa, Akiko ^a   Komano, Teruya ^a  

^a TOKYO METROPOLITAN UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ADHESIN; BACTERIUM LIPOPOLYSACCHARIDE; PROTEIN PILV; PROTEIN PILVA; PROTEIN PILVC; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENE; BACTERIUM PILUS; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; DNA RECOMBINATION; ESCHERICHIA COLI K 12; GENE SEQUENCE; LIPID MEMBRANE; MATING SYSTEM; NONHUMAN; PLASMID; PLASMID R64; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEIN LIPID INTERACTION; SALMONELLA ENTERICA; SEQUENCE ANALYSIS; WAA GENE; WILD TYPE;

BACTERIAL PROTEINS; BACTERIOPHAGES; CONJUGATION, GENETIC; DNA, BACTERIAL; ESCHERICHIA COLI; FIMBRIAE, BACTERIAL; LIPOPOLYSACCHARIDES; PLASMIDS; RECOMBINATION, GENETIC; SALMONELLA TYPHIMURIUM;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; ESCHERICHIA COLI K12; NEGIBACTERIA; SALMONELLA; SALMONELLA ENTERICA; SALMONELLA ENTERICA SUBSP. ENTERICA SEROVAR TYPHIMURIUM; UNIDENTIFIED BACTERIOPHAGE;

EID: 0042389587     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.185.17.5192-5199.2003     Document Type: Article

References (30)

1. Baxa, U., S. Steinbacher, S. Miller, A. Weintraub, R. Huber, and R. Seckler. 1996. Interactions of phage P22 tails with their cellular receptor, Salmonella O-antigen polysaccharide. Biophys. J. 71:2040-2048.
2. Feldman, M. F., C. L. Marolda, M. A. Monteiro, M. B. Perry, A. J. Parodi, and M. A. Valvano. 1999. The activity of a putative polyisoprenol-linked sugar translocase (Wzx) involved in Escherichia coli O antigen assembly is independent of the chemical structure of the O repeat. J. Biol. Chem. 274:35129-35138.
3. Gyohda, A., N. Furuya, N. Kogure, and T. Komano. 2002. Sequence-specific and non-specific binding of the Rci protein to the asymmetric recombination sites of the R64 shufflon. J. Mol. Biol. 318:975-983.
4. Hashimoto-Gotoh, T., F. C. Franklin, A. Nordheim, and K. N. Timmis. 1981. Specific-purpose plasmid cloning vectors. I. Low copy number, temperature-sensitive, mobilization-defective pSC101-derived containment vectors. Gene 16:227-235.
5. Heinrichs, D. E., J. A. Yethon, and C. Whitfield. 1998. Molecular basis for structural diversity in the core regions of the lipopolysaccharides of Escherichia coli and Salmonella enterica. Mol. Microbiol. 30:221-232.
6. Hitchcock, P. J., and T. M. Brown. 1983. Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J. Bacteriol. 154:269-277.
7. Hultgren, S. J., C. H. Jones, and S. Normark. 1996. Bacterial adhesins and their assembly, p. 2730-2756. In F. C. Neidhardt, R. Curtiss III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella: cellular and molecular biology, vol. 2. ASM Press, Washington, D.C.
8. Ishiwa, A., and T. Komano. 2000. The lipopolysaccharide of recipient cells is a specific receptor for PilV proteins, selected by shufflon DNA rearrangement, in liquid matings with donors bearing the R64 plasmid. Mol. Gen. Genet. 263:159-164.
9. Kim, S.-R., and T. Komano. 1997. The plasmid R64 thin pilus identified as a type IV pilus. J. Bacteriol. 179:3594-3603.
10. Klena, J. D., R. S. Ashford II, and C. A. Schnaitman. 1992. Role of Escherichia coli K-12 rfa genes and the rfp gene of Shigella dysenteriae 1 in generation of lipopolysaccharide core heterogeneity and attachment of O antigen. J. Bacteriol. 174:7297-7307.
11. Komano, T. 1999. Shufflons: multiple inversion systems and integrons. Annu. Rev. Genet. 33:171-191.
12. Komano, T., N. Funayama, S.-R. Kim, and T. Nisioka. 1990. Transfer region of IncI1 plasmid R64 and role of shufflon in R64 transfer. J. Bacteriol. 172:2230-2235.
13. Komano, T., S.-R. Kim, and T. Yoshida. 1995. Mating variation by DNA inversions of shufflon in plasmid R64. Adv. Biophys. 31:181-193.
14. Komano, T., S.-R. Kim, T. Yoshida, and T. Nisioka. 1994. DNA rearrangement of the shufflon determines recipient specificity in liquid mating of IncI1 plasmid R64. J. Mol. Biol. 243:6-9.
15. Komano, T., A. Kubo, and T. Nisioka. 1987. Shufflon: multi-inversion of four contiguous DNA segments of plasmid R64 creates seven different open reading frames. Nucleic Acids Res. 15:1165-1172.
16. Lerner, C. G., and M. Inouye. 1990. Low copy number plasmids for regulated low-level expression of cloned genes in Escherichia coli with blue/white insert screening capability. Nucleic Acids Res. 18:4631.
17. Liu, D., and R. Reeves. 1994. Escherichia coli regains its O antigen. Microbiology 140:49-57.
18. MacLachlan, P. R., S. K. Kadam, and K. E. Sanderson. 1991. Cloning, characterization, and DNA sequence of the rfaLK region for lipopolysaccharide synthesis in Salmonella typhimurium LT2. J. Bacteriol. 173:7151-7163.
19. Picken, R. N., and I. R. Beacham. 1977. Bacteriophage-resistant mutants of Escherichia coli K-12. Location of receptors within the lipopolysaccharide. J. Gen. Microbiol. 102:305-318.
20. Pradel, E., C. T. Parker, and C. A. Schnaitman. 1992. Structures of the rfaB, rfaI, rfaJ, and rfaS genes of Escherichia coli K-12 and their roles in assembly of the lipopolysaccharide core. J. Bacteriol. 174:4736-4745.
21. Pradel, E., and C. A. Schnaitman. 1991. Effect of rfaH (sfrB) and temperature on expression of rfa genes of Escherichia coli K-12. J. Bacteriol. 173: 6428-6431.
22. Raetz, C. R. H. 1996. Bacterial lipopolysaccharides: a remarkable family of bioactive macroamphiphiles, p. 1035-1063. In F. C. Neidhardt, R. Curtiss III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella: cellular and molecular biology, vol. 1. ASM Press, Washington, D.C.
23. Reeves, P. R., M. Hobbs, M. A. Valvano, M. Skurnik, C. Whitfield, D. Coplin, N. Kido, J. Klena, D. Maskell, C. R. Raetz, and P. D. Rick. 1996. Bacterial polysaccharide synthesis and gene nomenclature. Trends Microbiol. 4:495-503.
24. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
25. Schnaitman, C. A., and G. A. McDonald. 1984. Regulation of outer membrane protein synthesis in Escherichia coli K-12: deletion of ompC affects expression of the OmpF protein. J. Bacteriol. 159:555-563.
26. Steinbacher, S., S. Miller, U. Baxa, N. Budisa, A. Weintraub, R. Seckler, and R. Huber. 1997. Phage P22 tailspike protein: crystal structure of the head-binding domain at 2.3 Å, fully refined structure of the endorhamnosidase at 1.56 Å resolution, and the molecular basis of O-antigen recognition and cleavage. J. Mol. Biol. 267:865-880.
27. Vieira, J., and J. Messing. 1987. Production of single-stranded plasmid DNA. Methods Enzymol. 153:3-11.
28. Wilkinson, R. G., P. Gemski, Jr., and B. A. D. Stocker. 1972. Non-smooth mutants of Salmonella typhimurium: differentiation by phage sensitivity and genetic mapping. J. Gen. Microbiol. 70:527-554.
29. Yanisch-Perron, C., J. Vieira, and J. Messing. 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103-119.
30. Yoshida, T., N. Furuya, M. Ishikura, T. Isobe, K. Haino-Fukushima, T. Ogawa, and T. Komano. 1998. Purification and characterization of thin pili of IncI1 plasmids ColIb-P9 and R64: formation of PilV-specific cell aggregates by type IV pili. J. Bacteriol. 180:2842-2848.