Purification and Characterization of a New Type of α-Glucosidase from Paecilomyces lilacinus That Has Transglucosylation Activity to Produce α-1,3- and α-1,2-Linked Oligosaccharides

Bioscience, Biotechnology and Biochemistry

Volumn 67, Issue 1, 2003, Pages 29-35

  Kobayashi, Isao ^a   Tokuda, Masahiro ^a   Hashimoto, Hiroyuki ^b   Konda, Takashi ^c   Nakano, Hirofumi ^d   Kitahata, Sumio ^d  

^a J Oil Mills Inc   (Japan)

^b SHINSHU UNIVERSITY   (Japan)

^c KYOTO INSTITUTE OF TECHNOLOGY   (Japan)

^d OSAKA MUNICIPAL TECHNICAL RESEARCH INSTITUTE   (Japan)

Author keywords

Kojibiose; Nigerose; Paecilomyces lilacinus; Transglucosylation; glucosidase

Indexed keywords

FUNGI; PAECILOMYCES; PAECILOMYCES LILACINUS;

ALPHA GLUCOSIDASE; METAL; OLIGOSACCHARIDE;

ARTICLE; CARBOHYDRATE ANALYSIS; CHEMISTRY; CULTURE MEDIUM; ENZYME SPECIFICITY; GEL CHROMATOGRAPHY; GLYCOSYLATION; GROWTH, DEVELOPMENT AND AGING; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; MOLECULAR WEIGHT; PAECILOMYCES; PH; TEMPERATURE; THIN LAYER CHROMATOGRAPHY;

ALPHA-GLUCOSIDASES; CARBOHYDRATE SEQUENCE; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, ION EXCHANGE; CHROMATOGRAPHY, THIN LAYER; CULTURE MEDIA; GLYCOSYLATION; HYDROGEN-ION CONCENTRATION; METALS; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; OLIGOSACCHARIDES; PAECILOMYCES; SUBSTRATE SPECIFICITY; TEMPERATURE;

EID: 0042352394     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.67.29     Document Type: Article

References (18)

1. Tsujisaka, Y., and Fukumoto, J., Studies on maltose splitting enzymes of moulds. Part II. On the reaction mechanism of the crystalline transglucosidase of Aspergillus niger. Nippon Nogeikagaku Kaishi (in Japanese), 37, 747-752 (1963).
2. Yamasaki, Y., Suzuki, Y., and Ozawa, J., Purification and properties of a-glucosidase from Penicillium purpurogenum. Agric. Biol. Chem., 40, 669-676 (1976).
3. Chiba, S., Kimura, A., Kobori, T., and Saitoh, K., Quantitative determination of disaccharides produced from soluble starch through transglucosylation of buckwheat a-glucosidase. J. Jpn. Soc. Starch Sci., 32, 213-216 (1985).
4. Konishi, Y., and Shindo, K., Production of nigerose, nigerosyl glucose, and nigerosyl maltose by Acremo-nium sp. S4G13. Biosci. Biotechnol. Biochem., 61, 439-442 (1997).
5. Yamamoto, K., Urushibata, T., Konishi, Y., and Tsujii, M., Japan Kokai Tokkyo Koho, 1891001 (June 11, 2000).
6. Murosaki, S., Muroyama, K., Yamamoto, Y., Kusaka, H., Liu, T., and Yoshikai, Y., Im-munopotentiating activity of nigerooligosaccharides for the T helper 1-like immune response in mice. Biosci. Biotechnol. Biochem., 63, 373-378 (1999).
7. Murosaki, S., Muroyama, K., Yamamoto, Y., Liu, T., and Yoshikai, Y., Nigerooligosaccharides augments natural killer activity of hepatic mononuclear cells in mice. Int. Immunopharmacol., 2, 151-159 (2002).
8. Chaen, H., Yamamoto, T., Nishimoto, T., Nakada, T., Fukuda, S., Sugimoto, T., Kurimoto, M., and Tsujisaka, Y., Purification and characterization of a novel phosphorylase, kojibiose phosphorylase, from Thermoanaerobium brockii. J. Appl. Glycosci., 46, 423-429 (1999).
9. Shiota, M., and Yatake, T., Japan Tokkyo Koho, 3022958 (Jan. 31, 1991).
10. Chaen, H., Nishimoto, T., Nakada, T., Fukuda, S., Kurimoto, M., and Tsujisaka, Y., Enzymatic synthesis of kojioligosaccharides using kojibiose phos-phorylase. J. Biosci. Bioeng., 92, 177-182 (2001).
11. Kobayashi, I., Yasuno, S., Hashimoto, H., Ikura, K., Nakano, H., and Kitahata, S., Analysis of positional isomers of a-linked glucooligosaccharides by p-aminobenzoic ethyl ester-conversion method. J. Appl. Glycosci., 49, 461-468 (2002).
12. Yasuno, S., Murata, T., Kokubo, K., Yamaguchi, T., and Kamei, M., Two-mode analysis by high-performance liquid chromatography of p-aminobenzoic ethyl ester-derivatized monosaccharides. Biosci. Biotechnol. Biochem., 61, 1944-1946 (1997).
13. Reisfeld, R. A., Lewis, U. J., and Williams, D. E., Disk electrophoresis of basic proteins and properties of polyacrylamide gels. Nature, 195, 281-283 (1961).
14. Laemmli, U. K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685 (1970).
15. Lowry, O. H., Rosebrough, J. N., Farr, A. L., and Randall, R. J., Protein measurement with the Folin Phenol Reagent. J. Biol. Chem., 193, 265-275 (1951).
16. Konishi, Y., and Shindo, K., Japan Kokai Tokkyo Koho, 59559 (Mar. 7, 1995).
17. Sugawara, S., Nakamura, Y., and Shimomura, T., Substrate specificity and some properties of crystalline mold maltase. Agric. Biol. Chem., 25, 358-361 (1961).
18. Yamasaki, Y., Miyake, T., and Suzuki, Y., Properties of crystalline a-glucosidase from Mucor javani-cus. Agric. Biol. Chem., 37, 251-259 (1972).