Molecular analysis of the pediatric ocular surface for fungi

Current Eye Research

Volumn 26, Issue 1, 2003, Pages 33-36

  Wu, Tzu G ^a,b   Mitchell, Bradley M ^a,b   Carothers, Thomas S ^b,c   Coats, David K ^b,c   Brady McCreery, Kathryn M ^b,c   Paysse, Evelyn A ^b,c   Wilhelmus, Kirk R ^a,b  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

^b CULLEN EYE INSTITUTE   (United States)

^c TEXAS CHILDREN S HOSPITAL   (United States)

Author keywords

Candida albicans; Cornea; Fungal flora; Fungal prevalence; PCR

Indexed keywords

FUNGAL DNA; NUCLEIC ACID; POVIDONE IODINE; PRIMER DNA;

ADOLESCENT; ANTISEPSIS; ARTICLE; CANDIDA ALBICANS; CHILD; CLINICAL ARTICLE; CONJUNCTIVA; CONTROLLED STUDY; CORNEA; CORNEA SURGERY; DNA DETERMINATION; EXCISION; EXTRACTION; FEMALE; FUNGUS IDENTIFICATION; HUMAN; INFANT; MALE; MICROFLORA; PEDIATRICS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; STRABISMUS SURGERY; SURGICAL PATIENT; ADULT; AGING; DONOR; EYE; FUNGUS; GENETICS; ISOLATION AND PURIFICATION; MICROBIOLOGY; PRESCHOOL CHILD; SINGLE BLIND PROCEDURE;

ADOLESCENT; ADULT; AGING; CANDIDA ALBICANS; CHILD; CHILD, PRESCHOOL; CONJUNCTIVA; DNA, FUNGAL; EYE; FEMALE; FUNGI; HUMANS; MALE; POLYMERASE CHAIN REACTION; SINGLE-BLIND METHOD; TISSUE DONORS;

EID: 0042266466     PISSN: 02713683     EISSN: None     Source Type: Journal    
DOI: 10.1076/ceyr.26.1.33.14253     Document Type: Article

References (17)

1. Fazakas A. Über die von Bindehaut, Hornhaut, Lidrand, and Tranenwegengezuchteten, Pilze. [English translation] Albrecht Graefes Arch Ophthalmol. 1935;133:461-466.
2. Mitsui Y, Hanabusa J. Corneal infections after cortisone therapy. Br J Ophthalmol. 1955;39:244-250.
3. Hammeke JC, Ellis PP. Mycotic flora of the conjunctiva. Am J Ophthalmol. 1960;49:1174-1178.
4. Ainley R, Smith B. Fungal flora of the conjunctival sac in healthy and diseased eyes. Br J Ophthalmol. 1965;49:505-515.
5. Osato M. Normal ocular flora. In: Pepose JS, Holland GN, Wilhelmus KR, eds. Ocular Infection & Immunity. 2nd ed. St. Louis: Mosby; 1996:191-199.
6. Kercher L, Wardwell SA, Wilhelmus KR, Mitchell BM. Molecular screening of donor corneas for fungi prior to excision. Invest Ophthalmol Vis Sci. 2001;42:2578-2583.
7. Wahyuningsih R, Freisleben HJ, Sonntag HG, Schnitzler P. Simple and rapid detection of Candida albicans DNA in serum by PCR for diagnosis of invasive candidiasis. J Clin Microbiol. 2000;38:3016-3021.
8. Jaeger E, Carroll N, Choudhury S, Dunlop AA, Towler HM, Matheson MM, Adamson P, Okhravi N, Lightman S. Rapid detection and identification of Candida, Aspergillus, and Fusarium species in ocular samples using nested PCR. J Clin Microbiol. 2000;38:2902-2908.
9. Wu TG, Wilhelmus KR, Mitchell BM. Experimental keratomycosis in a mouse model. Invest Ophthalmol Vis Sci. 2003;44:(in press).
10. Okhravi N, Adamson P, Mant R, Matheson MM, Midgley G, Towler HM, Lightman S. Polymerase chain reaction and restriction fragment length polymorphism mediated detection and speciation of Candida spp. causing intraocular infection. Invest Ophthalmol Vis Sci. 1998;39:859-866.
11. Holly FJ, Lemp MA. Tear physiology and dry eyes. Surv Ophthalmol. 1977;22:69.
12. Selinger DS, Selinger RC, Reed WP. Resistance to infection of the external eye: The role of tears. Surv Ophthalmol. 1979;24:33-38.
13. Lemp MA, Blackman HJ. Ocular surface defense mechanism. Ann Ophthalmol. 1981;13:61-63.
14. Hirsch M, Prenant G, Renard G. Three-dimensional supramolecular organization of the extracellular matrix in human and rabbit corneal stroma, as revealed by ultrarapid-freezing and deep-etching methods. Exp Eye Res. 2001;72:123-135.
15. Pendrak ML, Krutzsch HC, Roberts DD. Structural requirements for hemoglobin to induce fibronectin receptor expression in Candida albicans. Biochemistry. 2000;39:16110-16118.
16. Kohno T, Sorgente N, Ishibashi T, Goodnight R, Ryan SJ. Immunofluorescent studies of fibronectin and laminin in the human eye. Invest Ophthalmol Vis Sci. 1987;28:506-514.
17. Puderbach S, Stolze HH. Tear ferning and other lacrimal tests in normal persons of different ages. Int Ophthalmol. 1991;15:391-395.