Detection of Trypanosoma cruzi DNA within Murine Cardiac Tissue Sections by in Situ Polymerase Chain Reaction

Memorias do Instituto Oswaldo Cruz

Volumn 98, Issue 3, 2003, Pages 373-376

  Lane, Joshua E ^a   Ribeiro Rodrigues, Rodrigo ^b   Olivares Villagómez, Danyvid ^c   Vnencak Jones, Cindy L ^d   McCurley, Thomas L ^d   Carter, Clint E ^c  

^a MEDICAL COLLEGE OF GEORGIA   (United States)

^b FEDERAL UNIVERSITY OF ESPÍRITO SANTO   (Brazil)

^c VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

^d VANDERBILT UNIVERSITY   (United States)

Author keywords

Direct in situ PCR; In situ polymerase chain reaction (PCR); Indirect in situ PCR; Murine cardiac tissue; Trypanosoma cruzi

Indexed keywords

DNA; RNA; KINETOPLAST DNA; PROTOZOAL DNA;

AMINO ACID SEQUENCE; ARTICLE; CARDIOVASCULAR SYSTEM; CHAGAS DISEASE; CONTROLLED STUDY; DNA SEQUENCE; IN SITU HYBRIDIZATION; NONHUMAN; POLYMERASE CHAIN REACTION; RNA SEQUENCE; TISSUE SECTION; TRYPANOSOMA CRUZI; ANIMAL; GENETICS; HEART; ISOLATION AND PURIFICATION; MOUSE; PARASITOLOGY;

MURINAE; TRYPANOSOMA; TRYPANOSOMA CRUZI;

ANIMALS; DNA, KINETOPLAST; DNA, PROTOZOAN; HEART; IN SITU HYBRIDIZATION; MICE; POLYMERASE CHAIN REACTION; TRYPANOSOMA CRUZI;

EID: 0042236848     PISSN: 00740276     EISSN: None     Source Type: Journal    
DOI: 10.1590/S0074-02762003000300013     Document Type: Article

References (21)

1. Boshoff C, Schulz TF, Kennedy MM, Graham AK, Fisher C, Thomas A, McGee JO, Weiss RA, O'Leary JJ 1995. Kaposi's sarcoma-associated herpesvirus infects endothelial and spindle cells. Nat Med 1: 1274-1278.
2. Brener Z 1973. Biology of Trypanosoma cruzi. Annu Rev Microbiol 27: 347-382.
3. Brener Z, Gazzinelli RT 1997. Immunological control of Trypanosoma cruzi infection and pathogenesis of Chagas' disease. Int Arch Allergy Immunol 114: 103-110.
4. Chen J, Rauch CA, White JH, Englund PT, Cozzarelli NR 1995. The topology of the kinetoplast DNA network. Cell 80: 61-69.
5. Degrave W, Fragos SP, Britto C, van Heuverswyn H, Kidane GZ, Cardoso MA, Mueller RU, Simpson L, Morel L 1998. Peculiar sequence organization of kinetoplast DNA minicircles from Trypanosoma cruzi. Mol Biochem Parasitol 27: 63-70.
6. Komminoth P, Long AA 1992. In-situ polymerase chain reaction. Virchows Archiv B Cell Pathol 64: 67-73.
7. Komminoth P, Long AA, Ray A, Wolfe HJ 1992. In situ polymerase chain reaction detection of viral DNA, single-copy genes, and gene rearrangements in cell suspensions and cytospins. Diag Mol Pathol 1: 85-97.
8. Lane JE, Olivares-Villagomez D, Vnencak-Jones, C, McCurley TL, Carter CE 1997. Detection of Trypanosoma cruzi with the polymerase chain reaction and in situ hybridization in infected murine cardiac tissue. Am J Trop Med Hyg 56: 588-595.
9. Larsson LI, Hougaard DM 1993. Sensitive detection of rat gastrin mRNA by in situ hybridization with chemically biotinylated oligodeoxynucleotides: Validation, quantitation, and double-staining studies. J Histochem Cytochem 41: 157-163.
10. Long AA, Komminoth P, Lee E, Wolfe HJ 1993. Comparison of indirect and direct in-situ polymerase chain reaction in cell preparations and tissue sections. Histochem 99: 151-162.
11. Moser DR, Kirchhoff LV, Donelson JE 1989. Detection of Trypanosoma cruzi by DNA amplification using the polymerase chain reaction. J Clin Microbiol 27: 1477-1482.
12. Nuovo GJ 1994. PCR in Situ: Protocols and Applications, 2nd ed., Raven Press, New York.
13. O'Leary JJ, Browne G, Johnson MI, Landers RJ, Crowley M, Healy I, Street JT, Pollock AM, Lewis FA, Andrew A 1994. PCR in situ hybridization detection of HPV 16 in fixed CaSki and fixed SiHa cell lines. J Clin Pathol 47: 933-938.
14. Olivares-Villagomez D, McCurley TL, Vnencak-Jones C, Correa-Oliveira R, Colley DG, Carter CE 1998. Polymerase chain reaction amplification of three different Trypanosoma cruzi DNA sequences from human chagasic cardiac tissue. Am J Trop Med Hyg 59: 563-570.
15. Palomino SA, Aiello VD, Higuchi ML 2000. Systematic mapping of hearts from chronic chagasic patients: the association between the occurrence of histopathological lesions and Trypanosoma cruzi antigens. Ann Trop Med Parasitol 94: 571-579.
16. Prata A 2001. Clinical and epidemiological aspects of Chagas disease. The Lancet Infectious Diseases 1: 92-100.
17. Sällström JF, Zehbe I, Alemi M, Wilander E 1993. Pitfalls of in situ polymerase chain reaction (PCR) using direct incorporation of labelled nucleotides. Anticancer Res 13: 1153-1154.
18. Tanowitz HB, Kirchoff LV, Simon D, Morris SA, Weiss LM, Wittner M 1992. Chagas' disease. Clin Microbiol Rev 5: 400-419.
19. Tarleton RL, Zhang L, Downs MO 1997. "Autoimmune rejection" of neonatal herat transplants in experimental Chagas disease is a parasite-specific response to infected host tissue. Proc Natl Acad Sci USA 94: 3932-3937.
20. Van Voorhis WC, Eisen H 1989. Fl-160. A surface antigen of Trypanosoma cruzi that mimics mammalian nervous tissue. J Exp Med 169: 641-652.
21. Van Voorhis WC, Schlekewy L, Trong HL 1991. Molecular mimicry by Trypanosoma cruzi: the Fl-160 epitope that mimics mammalian nerve can be mapped to a 12-amino acid peptide. Proc Natl Acad Sci USA 88: 5993-5997.